| Reference title | Date | Citation | Product usage |
|---|
| Electrochemical sensor for carbonyl groups in oxidized proteins | 2018 | Enache, T.A., Matei, E. and Diculescu, V.C., 2018. Electrochemical sensor for carbonyl groups in oxidized proteins. Analytical Chemistry. | …Electrophoretic measurements were performed on a multiSUB® Electrophoresis System from Cleaver Scientific Ltd, in a horizontal configuration and using a Consort EV202 power source… |
| Weed species within cereal crop rotations can serve as alternative hosts for Fusarium graminearum causing Fusarium head blight of wheat | 2019 | Suproniene, S., Kadziene, G., Irzykowski, W., Sneideris, D., Ivanauskas, A., Sakalauskas, S., Serbiak, P., Svegzda, P., Auskalniene, O. and Jedryczka, M., 2019. Weed species within cereal crop rotations can serve as alternative hosts for Fusarium graminearum causing Fusarium head blight of wheat. Fungal Ecology, 37, pp.30-37. | …60 s, followed by a final extension step at 72 °C for 10 min. Annealing temperature of 60 °C was used for trichothecene genotypes identification. Electrophoresis was run in a MultiSUB Mini apparatus (Cleaver Scientific Ltd, UK). PCR products were observed in 1% agarose gels using GeneRuler 100 bp DNA Latder (Thermo Fisher Scientific Baltics, Lithuania) as a marker.… |
| Biogenic amine and fermentation metabolite production assessments of Lactobacillus plantarum isolates for naturally fermented pickles | 2018 | Alan, Y., Topalcengiz, Z. and Dığrak, M., 2018. Biogenic amine and fermentation metabolite production assessments of Lactobacillus plantarum isolates for naturally fermented pickles. LWT. | …itial melting, followed by 30 cycles of denaturation at 94 °C for 1 min, annealing at 55 °C for 1 min, extension at 72 °C for 2 min, and a final elongation of 72 °C for 7 min. In gel electrophoresis (Cleaver Scientific Limited, United Kingdom), PCR products in aliquots were run on 2% agarose gel with 1X TBE buffer (90 mM Tris Base, 90 mM boric acid, 2 mM EDTA, pH 8.0; Sigma-Aldrich, USA) at 80 V f… |
| Cannabidiol effects on behaviour and immune gene expression in zebrafish (Danio rerio) | 2018 | Jensen, H.M., Korbut, R., Kania, P.W. and Buchmann, K., 2018. Cannabidiol effects on behaviour and immune gene expression in zebrafish (Danio rerio). PloS one, 13(7), p.e0200016. | …rk) was atded. RNA concentration was measured using a NanoDrop 2000 spectrophotometer (Saveen & Werner ApS, Denmark) and quality assessed by 1.5% ethidium bromide stained-agarose gel electrophoresis (Cleaver Scientific Ltd, UK). RNA samples were stored at -80°C until cDNA synthesis performed in Biometra T3 thermocycler (Fisher Scientific, Germany) using 1000ng of RNA, Oligo d(T)16 primers and Mult… |
| Human cystic echinococcosis in Turkey: a preliminary study on DNA polymorphisms of hydatid cysts removed from confirmed patients | 2018 | Orsten, S., Boufana, B., Ciftci, T. et al. Parasitol Res (2018) 117: 1257. https://doi.org/10.1007/s00436-018-5807-9 | …ed PCR products were visualised under ultraviolet light (Syngene G: Box gel documentation system, Cambridge Biosciences) following electrophoresis on 1.5% (w/v) ethidium bromide-stained agarose gels (Cleaver Scientific Limited) and purified using QIAquick PCR Purification Kit (Qiagen) according to the manufacturer’s instructions. Bi-directional commercial sequencing was achieved using the PCR prim… |
| An overlooked hybrid between the two diploid Chenopodium species in Central Europe determined by microsatellite and morphological analysis | 2018 | Hodková, E. & Mandák, B. Plant Syst Evol (2018) 304: 295. https://doi.org/10.1007/s00606-017-1477-9 | …ion (Online Resource 3), was isolated using the DNeasy kit 96 Plant Kit (Qiagen) following the manufacturer’s instructions. The quality and yield of the isolated DNA were checked on a 1% agarose gel (Cleaver Scientific Ltd) and then by precisely measuring DNA concentration and purity using a NanoDrop™ 2000 spectrophotometer (Thermo Fisher Scientific). All samples were diluted to 15–20 ng/μl prior … |
| Experimental Mycobacterium bovis infection in three white rhinoceroses (Ceratotherium simum): Susceptibility, clinical and anatomical pathology | 2017 | Michel AL, Lane EP, de Klerk-Lorist L-M, Hofmeyr M, van der Heijden EMDL, Botha L, et al. (2017) Experimental Mycobacterium bovis infection in three white rhinoceroses (Ceratotherium simum): Susceptibility, clinical and anatomical pathology. PLoS ONE 12(7): https://doi.org/10.1371/journal.pone.0179943 | …es of denaturation at 94°C (30 sec), annealing at 69°C (30 sec) and extension at 72°C (1 min) and final extension at 72°C for 10 min. PCR products were analysed using electrophoresis on a 2% agarose (Cleaver Scientific, England, UK) gel, stained with ethidium bromide (Invitrogen, Massachusetts, USA) and documented with the GelCompar Documentation System (Bio-Rad, California, USA).… |
| Identification and verification of porcine DNA in commercial gelatin and gelatin containing processed foods | 2017 | Hassan Ibrahem Abdullah Amqizal, Hassan Abdullah Al-Kahtani, Elsayed Ali Ismail, Khizar Hayat, Irwandi Jaswir, Food Control, Volume 78, August 2017, Pages 297-303, | … conventional PCR products were analyzed by electrophoresis in 2% agarose gel in trice boric acid-EDTA buffer, stained by acridine orange, and visualized after 25 min by 70 V electrophoresis machine (cleaver scientific Ltd. UK). The gel document was captured by Gel Doc™ EZ Imager, (BIO-RAD, USA). The fragments of positive samples were cut by scalpel and the amplicons were purified from the gel by … |
| Highly selective capture of minicircle DNA biopharmaceuticals by a novel zinc-histidine peptide conjugate | 2017 | Vítor M. Gaspar, Carla Cruz, João A. Queiroz, Chantal Pichon, Fani Sousa, Separation and Purification Technology, Volume 174, 1 March 2017, Pages 417-424, | …riefly, samples were initially injected in a 1% (w/v) low-melting agarose gel stained with Safe-green™ DNA gel stain (NZYTech, Lisbon, Portugal). Electrophoresis was performed in a horizontal system (cleaver scientific) at 90 V for 40 min. 1 kb DNA latder was used as control for DNA molecular weight. The gel was then imaged in a UV transilluminator equipped with a CCD camera for image acquisition … |
| Identification of biogenic amines-producing lactic acid bacteria isolated from spontaneous malolactic fermentation of chilean red wines | 2016 | Karem Henríquez-Aedo, Daniel Durán, Apolinaria Garcia, Martha B. Hengst, Mario Aranda, LWT – Food Science and Technology, Volume 68, May 2016, Pages 183-189, | …was carried out for 2 min at 72 °C. PCR products were separated by gel electrophoresis on 2% w/v agarose gel, prepared with 1X TAE (Tris acetate-EDTA, pH 8) buffer, in a MS Mini-7 horizontal chamber (cleaver scientific, Warwickshire, UK) applying 100V. PCR amplification patterns were visualized under a UV light using SafeView Nucleic Acid Stain (NBS Biologicals, Richmond, Canada) and 100 bp latder… |
| Designing multilayered nanoplatforms for SERS-based detection of genetically modified organisms | 2015 | Uluok, S., Guven, B., Eksi, H. et al. J Nanopart Res (2015) 17: 43. https://doi.org/10.1007/s11051-014-2849-5 | …The electrophoresis tank was provided by Cleaver Scientific Ltd. (Rugby, United Kingdom). A Micro-Bio-Tec Brand (Giessen, Germany) electrophoresis power supply was used, and the gels were monitored using the Kodak Gel Logic 200-Imaging System (New York, NY, USA). After nanoparticle synthesis, the absorption spectra were measured using an Agilent 8453 UV–Visible spectrophotometer (Agilent Technolog… |
| Coagulase-negative staphylococci (CoNS) isolated from ready-to-eat food of animal origin – Phenotypic and genotypic antibiotic resistance | 2015 | Wioleta Chajęcka-Wierzchowska, Anna Zadernowska, Beata Nalepa, Magda Sierpińska, Łucja Łaniewska-Trokenheim, Food Microbiology, Volume 46, April 2015, Pages 222-226, | …ncing and Oligonucleotide Synthesis (Institute of Biochemistry and Biophysics of the Polish Academy of Sciences in Warsaw, Poland) – Table 1. Electrophoretic separation: 100 V – 5 min; 70 V – 60 min (cleaver scientific, UK) was carried out on 1.5% agarose gel (Promega, Poland) with the atdition of ethidium bromide 0.5 μg/10 ml (MP Biomedicals, Poland). Positive controls included reference strains:… |
