Home News Reagents and Chemicals for Western Blotting

Reagents and Chemicals for Western Blotting

At Cleaver Scientific we offer the complete solution of reagents and chemicals for Western Blotting. To view the products individual product page, please click on the images.

Step 1- PAGE Buffers

Five buffers are available in powder sachets for a range of native and denaturing protein gel electrophoresis techniques. Convenient, pre-made stock solution or powder- just dilute or dissolve as necessary with water. Saves time and trouble- no weighing, pH adjustments or need to stock individual compounds.

PAGE buffers


Step 2- Protein Markers

Stable for up to two years if stored at -20°C and supplied pre-stained in gel loading buffer for direct loading, Cleaver Scientific PINK Plus and BLUE Wide range recombinant protein markers are perfect for SDS-PAGE applications.

Protein markers


Step 3- Blotting Membranes

PVDF with nitrocellulose (proteins) and nylon (RNA and DNA) membranes are available for different application needs and different formats. We supply membranes in sheet form and as a 3M roll which can be cut to size to fit experimental needs.

Blotting membranes

Step 4- Protein Gel Staining

EZEE RubyPro is a ready to use kit for rapid and sensitive protein staining of 1D and 2D SDS PAGE gels. It enables high contrast and optimal visualisation and quantitation of proteins.

EZEE UltraBlue is a sensitive, safe and environmentally friendly protein stain compatible with mass spectrometry. EZEE UltraBLUE is an enhanced Coomassie-based protein stain formulated for fast and sensitive protein detection without the use of hazardous chemicals.

Protein gel staining

Step 5- Blocking Buffer

JUSTBLOCK is an all-in-one blocking solution for Western blot analysis. By all-in-one we refer to its capability to perform in only one step, blocking, primary and secondary antibodies hybridization as well as enhancing the signal developed from HRP or AP substrates and therefore functions as both blocker and enhancer in Western analysis.

Blocking buffer

Step 6- ECL Substrates

The lumi range of ECL substrates are luminol-based enhanced chemiluminescent substrates which produce sensitive signals and are compatible with antibodies conjugated with horseradish peroxide (HRP).

ECL substrates

Step 7- Western Blot Imagers

All protein markers, pre cast gels and buffers work perfectly with our range of Western Blot Imagers. At Cleaver Scientific, we offer both an entry level chemiluminescence documentation system as well as a complete solution for chemiluminescence and fluorescent western blot imaging.

Western Blot Imagers


Newsletter Sign Up

  • Hidden
  • Hidden
  • Hidden
    DD slash MM slash YYYY
  • Hidden
  • Hidden
  • Hidden

Dear Customers

Cleaver Scientific Ltd is no longer trading and the Manufacturing Rights and IP have been acquired by Thistle Scientific Ltd. Cleaver Scientific Brand products are now available for purchase direct from Thistle Scientific, or via the various world wide distribution channels, with the same renowned customer service, and reliable delivery from Thistle Scientific 

For any questions please contact Thistle Scientific at: orders@thistlescientific.co.uk

Web Scientific brand logo

Web Scientific has now joined our sister site Thistle Scientific!

We’re the same great team with the same commitment to quality, but now you can browse a larger range with much greater ease and selling points to say why this is a benefit to you.