|Characterizing forensically important insect and microbial community colonization patterns in buried remains
||Iancu, L., Junkins, E.N., Necula-Petrareanu, G. and Purcarea, C., 2018. Characterizing forensically important insect and microbial community colonization patterns in buried remains. Scientific Reports, 8(1), p.15513.
||… (annealing), and 1 min at 72 °C (extension), 35 cycles of 30 s at 94 °C, 1.30 min at 51 °C, 1 min at 72 °C, and 5 min at 72 °C. The resulting DNA amplicons (710 bp) were analysed on 1% agarose gels (Cleaver Scientific, Rugby, UK), in the presence of a negative control. The PCR products were purified with PureLink PCR Purification Kit (ThermoFisher Scientific) and sent for sequencing to Macrogen G…
|Serratia strains isolated from the rhizosphere of raulí (Nothofagus alpina) in volcanic soils harbour PGPR mechanisms and promote raulí plantlet growth.
||Martínez, O.A., Encina, C., Tomckowiack, C., Droppelmann, F., Jara, R., Maldonado, C., Muñoz, O., García-Fraile, P. and Rivas, R., 2018. Serratia strains isolated from the rhizosphere of raulí (Nothofagus alpina) in volcanic soils harbour PGPR mechanisms and promote raulí plantlet growth. Journal of soil science and plant nutrition.
||…C for 10 min, followed by 30 cycles of 1 min at 94 °C, 1 min at 55 °C, and 1 min at 72 °C followed by a final extension of 10 min at 72 °C. PCR products were analysed on a 1% agarose gel (LE Agarose, Cleaver Scientific Ltd., USA) stained with GelRed™ (Biotium Inc., USA) and visualized under LED light.…
|Characterization and microbial analysis of first recorded observation of Conicera similis Haliday (Diptera: Phoridae) in forensic decomposition study in Romania
||Lavinia Iancu, Emily N. Junkins, Cristina Purcarea, Journal of Forensic and Legal Medicine, Volume 58, August 2018, Pages 50-55,
||…The amplicons were analyzed by electrophoresis on 1% agarose (cleaver scientific, England) gels. Subsequently, the PCR products were purified with PureLink® PCR Purification Kit (Invitrogen, USA) and sequenced (Macrogen, Netherlands).…
|Development of quantum-dot-encapsulated liposome-based optical nanobiosensor for detection of telomerase activity without target amplification
||Zavari-Nematabad, A., Alizadeh-Ghodsi, M., Hamishehkar, H. et al. Anal Bioanal Chem (2017) 409: 1301. https://doi.org/10.1007/s00216-016-0058-z
||…control, the cell extracts were pre-treated by heat at 95 °C for 15 min. Therefore, telomerase was inactivated by heat shock. Finally, PCR products were separated by agarose (2%) gel electrophoresis (Cleaver Scientific Ltd, UK).…
|Retrotransposon distribution and copy number variation in gymnosperm genomes
||Voronova, A., Belevich, V., Korica, A. et al. Tree Genetics & Genomes (2017) 13: 88. https://doi.org/10.1007/s11295-017-1165-5
||…72 °C for 40 s, and a final elongation step at 72 °C for 10 min. Amplification products were separated by electrophoresis at 94 V for 4 h in 1× TAE buffer in a 1.5% agarose gel (LE Agarose CSL-AG500, Cleaver Scientific Ltd) with ethidium bromide. For validation, some ambiguous fragments slightly differing in length from the expected size range, or present in phylogenetically distant species, were …
|First report of Fusarium boothii from pecan (Carya illinoinensis) and camel thorn (Vachellia erioloba) trees in South Africa
||M. Gryzenhout, B. Khooa, L. Landman, South African Journal of Botany, Volume 105, July 2016, Pages 158-162,
||…in genes were obtained following the protocols of O’Donnell et al., 2000, O’Donnell et al., 2004 using the Robust PCR kit (KAPA Biosystems). Amplification products were visualized on 1% agarose gels (cleaver scientific, AEC-Amersham, South Africa) containing Gelred DNA stain (Biotium, Anatech, South Africa) under UV illumination using a Geldoc XR + imaging system (Bio-Rad, South Africa).…
|Using bacterial and necrophagous insect dynamics for post-mortem interval estimation during cold season: Novel case study in Romania
||Lavinia Iancu, David O. Carter, Emily N. Junkins, Cristina Purcarea, Forensic Science International, Volume 254, September 2015, Pages 106-117,
||…a final extension step of 5 min at 72 °C. A negative control for DNA amplification was carried out in the absence of insect DNA. The amplicons (710 bp) were analyzed by electrophoresis on 1% agarose (cleaver scientific, England) gels. After purification with QIAquick PCR Purification Kit (Qiagen), the DNA fragments were sequenced on both strands, using the cloning primers (Macrogen, Netherlands).…