Semi Dry Blotter

£428.00£1,551.00

Key Features

  • Rapid transfer times
  • Western, Southern and Northern Blots
  • Economic Transfers due to very low buffer volumes
  • Screw down lid
  • Gels from 0.25 up to 10mm thick can be blotted
  • Uniform heat dispersion
  • Long life electrodes
SKU: SEMIDRY Category:
Special Requirements?

Description

These Semi Dry Blotters offer rapid transfer times for DNA, RNA and protein blotting typically 15 to 30 minutes. All units can be used for all types of blotting: Western, Southern and Northern via uncomplicated buffer and set up procedures and are compatible with gel thicknesses from 0.25 up to 10mm without the need for additional equipment. Each unit is compatible with their respective omniPAGE vertical gel system.

Semi Dry Blotting has the added benefit of economic transfers. Due to very low buffer volumes typically only a few millilitres of buffer are required per transfer. These Semi-Dry Blotters utilise a screw down lid, which secures the blot sandwich and allows complete control of pressure ensuring even transfer. The electrodes, comprising platinum coated anode and stainless steel cathode, will exhibit practically no corrosion and so provide many years of trouble free use.

Uniform heat dispersion across the blot sandwich ensures stable transfer times and no heat induced sample loss or transfer distortions. Being translucent, it allows viewing of the blot sandwich to ensure correct positioning and transfer is occurring correctly. Electrode plates are fully separated to prevent arcing or damage.

Catalogue Page Number: 45

This unit has a 12 month limited warranty, for more information, consult our terms and conditions.

SKUSD10SD10-PP3AMPSD20SD20-PP3AMP
Unit Dimensions (cm)16.5 x 15 x 5.5Yes26 x 26 x 7Yes
Capacity10 x 10 cmWestern Blotting20 x 20 cmWestern Blotting
Base Buffer Volume20POWERPRO3AMP, SD105POWERPRO3AMP, SD20
Blotter TypeSemi-DrySemi-Dry

This product has been cited in the following research papers:

Reference title Date Citation Product usage
The Role of Calcium in Differentiation of Human Adipose-Derived Stem Cells to Adipocytes 2018 Goudarzi, F., Mohammadalipour, A., Khodadadi, I. et al. Mol Biotechnol (2018) 60: 279. https://doi.org/10.1007/s12033-018-0071-x …by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) in 10% gel under reducing conditions and electroblotted onto nitrocellulose membrane by a semidry transfer membrane instrument (Cleaver Scientific, Warwickshire, UK). Then, following washing the membrane by TBS buffer (20 mM Tris.Hcl, 500 mM NaCl, pH 7.4), they were blocked by 3% BSA prepared in TBST buffer (20 mM Tris.Hcl, 50…
Development of Adenosine Deaminase-Specific IgY Antibodies: Diagnostic and Inhibitory Application 2018 Łupicka-Słowik, A., Psurski, M., Grzywa, R. et al. Appl Biochem Biotechnol (2018) 184: 1358. https://doi.org/10.1007/s12010-017-2626-x …ific IgYs and increased antibody avidity. For Western blot assay, calf ADA (50 ng/lane) was resolved by SDS-PAGE (4–12%, Tris-glycine) under reducing conditions and blotted (semi-dry blotting system, Cleaver Scientific, Rugby, UK) onto nitrocellulose membrane (0.45 μm, Thermo Scientific, Gdańsk, Poland). The membrane was blocked with 5% skim milk in 10 mM phosphate-buffered saline with 0.05% Tween…
Discovering simple phenylboronic acid and benzoxaborole derivatives for experimental oncology – phase cycle-specific inducers of apoptosis in A2780 ovarian cancer cells 2018 Psurski, M., Łupicka-Słowik, A., Adamczyk-Woźniak, A. et al. Invest New Drugs (2018). https://doi.org/10.1007/s10637-018-0611-z …SDS-PAGE (4–12%, Tris-glycine) under reducing conditions and transferred onto a nitrocellulose membrane (pore size 0.45 μm; Thermo Fisher Scientific, Warsaw, Poland) using a semi-dry blotting system (Cleaver Scientific, Rugby, UK). The membrane was washed with Tris-buffered saline (TBS, 20 mM Tris-Base, 137 mM NaCl, pH 7.6; 5 min, room temperature) and blocked with 5% skim milk in TBS with 0.1% Tw…
MiR-132 regulated olfactory bulb proteins linked to olfactory learning in greater short-nosed fruit bat Cynopterus sphinx 2018 Murugan Mukilan, David Mary Rajathei, Edwin Jeyaraj, Nagarajan Kayalvizhi, Koilmani Emmanuvel Rajan, Gene, Volume 671, 10 September 2018, Pages 10-20, …Equal concentrations (30 μg) of total proteins were resolved on 10% SDS-polyacrylamide gel and a semi-dry western apparatus (SD 20; cleaver scientific Ltd., UK) was used to transfer the separated proteins to polyvinylidene difluoride (PVDF) membrane (Millipore India Pvt. Ltd., India). Initially, membranes were blocked with 2% BSA for 3 h and then incubated with specific primary antibodies [Rabbit …
Effect of human papillomavirus 16 oncoproteins on oncostatin M upregulation in oral squamous cell carcinoma 2016 Chuerduangphui, J., Pientong, C., Chaiyarit, P. et al. Med Oncol (2016) 33: 83. https://doi.org/10.1007/s12032-016-0800-6 …ord assay (Bio-Rad, Richmond, CA, USA) and separated on SDS-PAGE (12 % gel). The proteins were transferred onto nitrocellulose membranes (Bio-Rad, Hercules, CA, USA) using a semi-dry blotting system (Cleaver Scientific Ltd, UK). The membrane was incubated in blocking buffer, and primary antibody [mouse anti-p53 (Santa Cruz, CA, USA), mouse anti-β-actin (Sigma) or rabbit anti-OSM (Sigma)] was used …
Environmental Enrichment Reduces Anxiety by Differentially Activating Serotonergic and Neuropeptide Y (NPY)-Ergic System in Indian Field Mouse (Mus booduga): An Animal Model of Post-Traumatic Stress Disorder 2015 Ragu Varman D, Rajan KE (2015) Environmental Enrichment Reduces Anxiety by Differentially Activating Serotonergic and Neuropeptide Y (NPY)-Ergic System in Indian Field Mouse (Mus booduga): An Animal Model of Post-Traumatic Stress Disorder. PLoS ONE 10(5):
e0127945.

https://doi.org/10.1371/journal.pone.0127945

…lved on SDS-polyacrylamide gel. The separated proteins were transferred electrophoretically onto polyvinylidene difluoride (PVDF) membrane (Millipore India) using a semi-dry western apparatus (SD 20; Cleaver Scientific Ltd, UK). The membranes were blocked and incubated at 4°C for 9 h with one of the following specific primary antibodies (Santa-Cruz Biotechnology, Germany and Upstate, Cell signalin…
Recombinant expression of the alternate reading frame protein (ARFP) of hepatitis C virus genotype 4a (HCV-4a) and detection of ARFP and anti-ARFP antibodies in HCV-infected patients 2015 Shehat, M.G., Bahey-El-Din, M., Kassem, M.A. et al. Arch Virol (2015) 160: 1939. https://doi.org/10.1007/s00705-015-2465-4 …purified recombinant ARFP and also total lysate of induced E. coli M15 (pREP4) (pQE31-ARFP) was blotted against a nitrocellulose membrane (Life Technologies, USA) using a semi-dry blotting apparatus (Cleaver Scientific, UK). After protein transfer, the membrane was left overnight in blocking buffer composed of 5 % skimmed milk in TBS buffer (0.8 % sodium chloride, 20 mM Tris-HCl, pH 7.6) at 4 °C. …
Differential expression patterns among heat-shock protein genes and thermal responses in the whitefly Bemisia tabaci (MEAM 1) 2015 Fernando Díaz, Rony F. Orobio, Paul Chavarriaga, Nelson Toro-Perea, Journal of Thermal Biology, Volume 52, August 2015, Pages 199-207, …ambrook et al. (2001) in the Mini-PROTEAN® Tetra System (Bio-Rad). Samples were transferred electrophoretically to a polyvinylidene difluoride (PVDF) membrane in the SD10 mini semi-dry blotting unit (cleaver scientific) at 200 mA/30 min. The membrane was blocked with RapidBlock™ solution (AMRESCO) and then incubated with one of a number of primary antibodies tested to detect HSP70 or HSP90 protein…
Activity-dependent expression of miR-132 regulates immediate-early gene induction during olfactory learning in the greater short-nosed fruit bat, Cynopterus sphinx 2015 Murugan Mukilan, Durairaj Ragu Varman, Sivasubramaniam Sudhakar, Koilmani Emmanuvel Rajan, Neurobiology of Learning and Memory, Volume 120, April 2015, Pages 41-51, …DS–polyacrylamide gel. The separated proteins were transferred electrophoretically onto a polyvinylidene difluoride (PVDF) membrane (Millipore India P. Ltd) using a semi-dry western apparatus (SD 20; cleaver scientific Ltd, UK). The membranes were blocked and incubated at 4 °C for 8 h with one of the following specific primary antibodies (Santa-Cruz Biotechnology and Calbiochem): affinity-purified…
Neuroprotective and Anti-Apoptotic Propensity of Bacopa monniera Extract Against Sodium Nitroprusside Induced Activation of iNOS, Heat Shock Proteins and Apoptotic Markers in PC12 Cells 2014 Pandareesh, M.D. & Anand, T. Neurochem Res (2014) 39: 800. https://doi.org/10.1007/s11064-014-1273-7 …Total cellular protein, mitochondrial and cytosolic protein fractions were separated on SDS-PAGE and transferred onto a nitrocellulose membrane using an electro blotting apparatus (Cleaver Scientific Ltd., UK) as per our earlier studies [11]. After transfer, the membranes were probed with α-Tubulin B-7 (sc-5286), HSP-70 C92F3A (sc-66048), Bcl-2 (sc-7382), Bax (sc-70408), cytochrome c (sc-13156) (S…
Anti-apoptotic mechanism of Bacoside rich extract against reactive nitrogen species induced activation of iNOS/Bax/caspase 3 mediated apoptosis in L132 cell line 2014 Anand, T., Pandareesh, M.D., Bhat, P.V. et al. Cytotechnology (2014) 66: 823. https://doi.org/10.1007/s10616-013-9634-7 …Total cellular protein was separated on SDS-PAGE and transferred onto a nitrocellulose membrane using an electro blotting apparatus (Cleaver Scientific Ltd, Rugby, UK) as per earlier method (Anand et al. 2012). After transfer, the membranes were probed with α-tubulin B-7 (sc-5286), anti HSP-70 C92F3A (sc-66048), Bcl2 (sc-7382), Bax (sc-70408), cytochrome c (sc-13156) (Santa Cruz Biotechnology, San…
Environmental enrichment modulates glucocorticoid receptor expression and reduces anxiety in Indian field male mouse Mus booduga through up-regulation of microRNA-124a 2014 Ragu Varman Durairaj, Emmanuvel Rajan Koilmani, General and Comparative Endocrinology, Volume 199, 1 April 2014, Pages 26-32, …cyl sulfate–polyacrylamide gel. The separated proteins were transferred electrophoretically onto polyvinylidene difluoride (PVDF) membrane (Millipore India) using a semi-dry western apparatus (SD 20; cleaver scientific Ltd, UK). The membranes were then preblocked with Tris-buffered saline [10 mM Tris-base pH-7.5, 150 mM Nacl (TBS) containing non fat dry milk (5%) and tween 20 (0.1%)] for 3 h at RT…
Effects of heavy metals on antioxidants and expression of HSP70 in different tissues of Milk fish (Chanos chanos) of Kaattuppalli Island, Chennai, India 2013 Sivakumar Rajeshkumar, Jayaprakash Mini, Natesan Munuswamy, Ecotoxicology and Environmental Safety, Volume 98, 1 December 2013, Pages 8-18, … (Pall Corporation, East Hills, New York) at 15 V for 90 min using alkaline buffer (48 mM Tris, 39 mM glycine, 0.037 percent (w/v) SDS, 20 percent methanol v/v, pH 8.3) and a semi dry blotting units (cleaver scientific Ltd., Rugby, UK). The PVDF blots were blocked with TBST (10 mM Tris, 150 mM NaCl, 1.54 mM sodium azide, 0.05 percent (v/v) Tween-20, pH 7.5) containing 5 percent (w/v) skimmed milk …
Heat-shock protein 90 alpha (HSP90α) modulates signaling pathways towards tolerance of oxidative stress and enhanced survival of hepatocytes of Mugil cephalus 2011 Padmini, E. & Usha Rani, M. Cell Stress and Chaperones (2011) 16: 411. https://doi.org/10.1007/s12192-011-0255-9 … μm pore size (Pall Corporation, East Hills, New York, USA) at 15 V for 90 min using alkaline buffer (48 mM Tris, 39 mM glycine, 0.037% w/v SDS, 20% v/v methanol, pH 8.3) and semi-dry blotting units (Cleaver Scientific Ltd., Rugby, UK). The PVDF blots were blocked with TBST (10 mM Tris, 150 mM NaCl, 1.54 mM sodium azide, 0.05% v/v Tween-20, pH 7.5) containing 5% w/v skimmed milk powder for 3 h, fo…
Local RNA Silencing Mediated by Agroinfiltration 2011 Cite this protocol as: Helm J.M., Dadami E., Kalantidis K. (2011) Local RNA Silencing Mediated by Agroinfiltration. In: Kodama H., Komamine A. (eds) RNAi and Plant Gene Function Analysis. Methods in Molecular Biology (Methods and Protocols), vol 744. Humana Press …Blotting: SD20 Semi Dry Midi (Cleaver Scientific Ltd, Warwickshire, UK).…
Expression and structural characterization of peripherin/RDS, a membrane protein implicated in photoreceptor outer segment morphology 2010 Vos, W.L., Vaughan, S., Lall, P.Y. et al. Eur Biophys J (2010) 39: 679. https://doi.org/10.1007/s00249-009-0553-7 …Proteins were transferred to PVDF membrane (Amersham) using a transfer unit for semi-dry transfer (Cleaver Scientific) according to the manufacturer’s instructions. Western blots were probed with c-myc tag mouse monoclonal antibody (Cell Signaling), using anti-mouse HRP conjugated antibody as the secondary (Promega). Mass spectrometry analysis was performed on a an LC/MS system consisting of a 1,2…

Accessories…

Semi dry Blotter Cables (Red & Black)
CSL-SDCAB £40.00
10cm acrylic roller for western blotting
CSL-WBROLLER £25.00