| Reference title | Date | Citation | Product usage |
|---|
| The Role of Calcium in Differentiation of Human Adipose-Derived Stem Cells to Adipocytes | 2018 | Goudarzi, F., Mohammadalipour, A., Khodadadi, I. et al. Mol Biotechnol (2018) 60: 279. https://doi.org/10.1007/s12033-018-0071-x | …by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) in 10% gel under reducing conditions and electroblotted onto nitrocellulose membrane by a semidry transfer membrane instrument (Cleaver Scientific, Warwickshire, UK). Then, following washing the membrane by TBS buffer (20 mM Tris.Hcl, 500 mM NaCl, pH 7.4), they were blocked by 3% BSA prepared in TBST buffer (20 mM Tris.Hcl, 50… |
| Development of Adenosine Deaminase-Specific IgY Antibodies: Diagnostic and Inhibitory Application | 2018 | Łupicka-Słowik, A., Psurski, M., Grzywa, R. et al. Appl Biochem Biotechnol (2018) 184: 1358. https://doi.org/10.1007/s12010-017-2626-x | …ific IgYs and increased antibody avidity. For Western blot assay, calf ADA (50 ng/lane) was resolved by SDS-PAGE (4–12%, Tris-glycine) under reducing conditions and blotted (semi-dry blotting system, Cleaver Scientific, Rugby, UK) onto nitrocellulose membrane (0.45 μm, Thermo Scientific, Gdańsk, Poland). The membrane was blocked with 5% skim milk in 10 mM phosphate-buffered saline with 0.05% Tween… |
| Discovering simple phenylboronic acid and benzoxaborole derivatives for experimental oncology – phase cycle-specific inducers of apoptosis in A2780 ovarian cancer cells | 2018 | Psurski, M., Łupicka-Słowik, A., Adamczyk-Woźniak, A. et al. Invest New Drugs (2018). https://doi.org/10.1007/s10637-018-0611-z | …SDS-PAGE (4–12%, Tris-glycine) under reducing conditions and transferred onto a nitrocellulose membrane (pore size 0.45 μm; Thermo Fisher Scientific, Warsaw, Poland) using a semi-dry blotting system (Cleaver Scientific, Rugby, UK). The membrane was washed with Tris-buffered saline (TBS, 20 mM Tris-Base, 137 mM NaCl, pH 7.6; 5 min, room temperature) and blocked with 5% skim milk in TBS with 0.1% Tw… |
| MiR-132 regulated olfactory bulb proteins linked to olfactory learning in greater short-nosed fruit bat Cynopterus sphinx | 2018 | Murugan Mukilan, David Mary Rajathei, Edwin Jeyaraj, Nagarajan Kayalvizhi, Koilmani Emmanuvel Rajan, Gene, Volume 671, 10 September 2018, Pages 10-20, | …Equal concentrations (30 μg) of total proteins were resolved on 10% SDS-polyacrylamide gel and a semi-dry western apparatus (SD 20; cleaver scientific Ltd., UK) was used to transfer the separated proteins to polyvinylidene difluoride (PVDF) membrane (Millipore India Pvt. Ltd., India). Initially, membranes were blocked with 2% BSA for 3 h and then incubated with specific primary antibodies [Rabbit … |
| Effect of human papillomavirus 16 oncoproteins on oncostatin M upregulation in oral squamous cell carcinoma | 2016 | Chuerduangphui, J., Pientong, C., Chaiyarit, P. et al. Med Oncol (2016) 33: 83. https://doi.org/10.1007/s12032-016-0800-6 | …ord assay (Bio-Rad, Richmond, CA, USA) and separated on SDS-PAGE (12 % gel). The proteins were transferred onto nitrocellulose membranes (Bio-Rad, Hercules, CA, USA) using a semi-dry blotting system (Cleaver Scientific Ltd, UK). The membrane was incubated in blocking buffer, and primary antibody [mouse anti-p53 (Santa Cruz, CA, USA), mouse anti-β-actin (Sigma) or rabbit anti-OSM (Sigma)] was used … |
| Environmental Enrichment Reduces Anxiety by Differentially Activating Serotonergic and Neuropeptide Y (NPY)-Ergic System in Indian Field Mouse (Mus booduga): An Animal Model of Post-Traumatic Stress Disorder | 2015 | Ragu Varman D, Rajan KE (2015) Environmental Enrichment Reduces Anxiety by Differentially Activating Serotonergic and Neuropeptide Y (NPY)-Ergic System in Indian Field Mouse (Mus booduga): An Animal Model of Post-Traumatic Stress Disorder. PLoS ONE 10(5):
e0127945. https://doi.org/10.1371/journal.pone.0127945 | …lved on SDS-polyacrylamide gel. The separated proteins were transferred electrophoretically onto polyvinylidene difluoride (PVDF) membrane (Millipore India) using a semi-dry western apparatus (SD 20; Cleaver Scientific Ltd, UK). The membranes were blocked and incubated at 4°C for 9 h with one of the following specific primary antibodies (Santa-Cruz Biotechnology, Germany and Upstate, Cell signalin… |
| Recombinant expression of the alternate reading frame protein (ARFP) of hepatitis C virus genotype 4a (HCV-4a) and detection of ARFP and anti-ARFP antibodies in HCV-infected patients | 2015 | Shehat, M.G., Bahey-El-Din, M., Kassem, M.A. et al. Arch Virol (2015) 160: 1939. https://doi.org/10.1007/s00705-015-2465-4 | …purified recombinant ARFP and also total lysate of induced E. coli M15 (pREP4) (pQE31-ARFP) was blotted against a nitrocellulose membrane (Life Technologies, USA) using a semi-dry blotting apparatus (Cleaver Scientific, UK). After protein transfer, the membrane was left overnight in blocking buffer composed of 5 % skimmed milk in TBS buffer (0.8 % sodium chloride, 20 mM Tris-HCl, pH 7.6) at 4 °C. … |
| Differential expression patterns among heat-shock protein genes and thermal responses in the whitefly Bemisia tabaci (MEAM 1) | 2015 | Fernando Díaz, Rony F. Orobio, Paul Chavarriaga, Nelson Toro-Perea, Journal of Thermal Biology, Volume 52, August 2015, Pages 199-207, | …ambrook et al. (2001) in the Mini-PROTEAN® Tetra System (Bio-Rad). Samples were transferred electrophoretically to a polyvinylidene difluoride (PVDF) membrane in the SD10 mini semi-dry blotting unit (cleaver scientific) at 200 mA/30 min. The membrane was blocked with RapidBlock™ solution (AMRESCO) and then incubated with one of a number of primary antibodies tested to detect HSP70 or HSP90 protein… |
| Activity-dependent expression of miR-132 regulates immediate-early gene induction during olfactory learning in the greater short-nosed fruit bat, Cynopterus sphinx | 2015 | Murugan Mukilan, Durairaj Ragu Varman, Sivasubramaniam Sudhakar, Koilmani Emmanuvel Rajan, Neurobiology of Learning and Memory, Volume 120, April 2015, Pages 41-51, | …DS–polyacrylamide gel. The separated proteins were transferred electrophoretically onto a polyvinylidene difluoride (PVDF) membrane (Millipore India P. Ltd) using a semi-dry western apparatus (SD 20; cleaver scientific Ltd, UK). The membranes were blocked and incubated at 4 °C for 8 h with one of the following specific primary antibodies (Santa-Cruz Biotechnology and Calbiochem): affinity-purified… |
| Neuroprotective and Anti-Apoptotic Propensity of Bacopa monniera Extract Against Sodium Nitroprusside Induced Activation of iNOS, Heat Shock Proteins and Apoptotic Markers in PC12 Cells | 2014 | Pandareesh, M.D. & Anand, T. Neurochem Res (2014) 39: 800. https://doi.org/10.1007/s11064-014-1273-7 | …Total cellular protein, mitochondrial and cytosolic protein fractions were separated on SDS-PAGE and transferred onto a nitrocellulose membrane using an electro blotting apparatus (Cleaver Scientific Ltd., UK) as per our earlier studies [11]. After transfer, the membranes were probed with α-Tubulin B-7 (sc-5286), HSP-70 C92F3A (sc-66048), Bcl-2 (sc-7382), Bax (sc-70408), cytochrome c (sc-13156) (S… |
| Anti-apoptotic mechanism of Bacoside rich extract against reactive nitrogen species induced activation of iNOS/Bax/caspase 3 mediated apoptosis in L132 cell line | 2014 | Anand, T., Pandareesh, M.D., Bhat, P.V. et al. Cytotechnology (2014) 66: 823. https://doi.org/10.1007/s10616-013-9634-7 | …Total cellular protein was separated on SDS-PAGE and transferred onto a nitrocellulose membrane using an electro blotting apparatus (Cleaver Scientific Ltd, Rugby, UK) as per earlier method (Anand et al. 2012). After transfer, the membranes were probed with α-tubulin B-7 (sc-5286), anti HSP-70 C92F3A (sc-66048), Bcl2 (sc-7382), Bax (sc-70408), cytochrome c (sc-13156) (Santa Cruz Biotechnology, San… |
| Environmental enrichment modulates glucocorticoid receptor expression and reduces anxiety in Indian field male mouse Mus booduga through up-regulation of microRNA-124a | 2014 | Ragu Varman Durairaj, Emmanuvel Rajan Koilmani, General and Comparative Endocrinology, Volume 199, 1 April 2014, Pages 26-32, | …cyl sulfate–polyacrylamide gel. The separated proteins were transferred electrophoretically onto polyvinylidene difluoride (PVDF) membrane (Millipore India) using a semi-dry western apparatus (SD 20; cleaver scientific Ltd, UK). The membranes were then preblocked with Tris-buffered saline [10 mM Tris-base pH-7.5, 150 mM Nacl (TBS) containing non fat dry milk (5%) and tween 20 (0.1%)] for 3 h at RT… |
| Effects of heavy metals on antioxidants and expression of HSP70 in different tissues of Milk fish (Chanos chanos) of Kaattuppalli Island, Chennai, India | 2013 | Sivakumar Rajeshkumar, Jayaprakash Mini, Natesan Munuswamy, Ecotoxicology and Environmental Safety, Volume 98, 1 December 2013, Pages 8-18, | … (Pall Corporation, East Hills, New York) at 15 V for 90 min using alkaline buffer (48 mM Tris, 39 mM glycine, 0.037 percent (w/v) SDS, 20 percent methanol v/v, pH 8.3) and a semi dry blotting units (cleaver scientific Ltd., Rugby, UK). The PVDF blots were blocked with TBST (10 mM Tris, 150 mM NaCl, 1.54 mM sodium azide, 0.05 percent (v/v) Tween-20, pH 7.5) containing 5 percent (w/v) skimmed milk … |
| Heat-shock protein 90 alpha (HSP90α) modulates signaling pathways towards tolerance of oxidative stress and enhanced survival of hepatocytes of Mugil cephalus | 2011 | Padmini, E. & Usha Rani, M. Cell Stress and Chaperones (2011) 16: 411. https://doi.org/10.1007/s12192-011-0255-9 | … μm pore size (Pall Corporation, East Hills, New York, USA) at 15 V for 90 min using alkaline buffer (48 mM Tris, 39 mM glycine, 0.037% w/v SDS, 20% v/v methanol, pH 8.3) and semi-dry blotting units (Cleaver Scientific Ltd., Rugby, UK). The PVDF blots were blocked with TBST (10 mM Tris, 150 mM NaCl, 1.54 mM sodium azide, 0.05% v/v Tween-20, pH 7.5) containing 5% w/v skimmed milk powder for 3 h, fo… |
| Local RNA Silencing Mediated by Agroinfiltration | 2011 | Cite this protocol as: Helm J.M., Dadami E., Kalantidis K. (2011) Local RNA Silencing Mediated by Agroinfiltration. In: Kodama H., Komamine A. (eds) RNAi and Plant Gene Function Analysis. Methods in Molecular Biology (Methods and Protocols), vol 744. Humana Press | …Blotting: SD20 Semi Dry Midi (Cleaver Scientific Ltd, Warwickshire, UK).… |
| Expression and structural characterization of peripherin/RDS, a membrane protein implicated in photoreceptor outer segment morphology | 2010 | Vos, W.L., Vaughan, S., Lall, P.Y. et al. Eur Biophys J (2010) 39: 679. https://doi.org/10.1007/s00249-009-0553-7 | …Proteins were transferred to PVDF membrane (Amersham) using a transfer unit for semi-dry transfer (Cleaver Scientific) according to the manufacturer’s instructions. Western blots were probed with c-myc tag mouse monoclonal antibody (Cell Signaling), using anti-mouse HRP conjugated antibody as the secondary (Promega). Mass spectrometry analysis was performed on a an LC/MS system consisting of a 1,2… |
