proSET Evo Fermentation System

Key Features

  • Duo heating system, thermostat and dry heating combined in one
  • Universal system for R&D in cell culture and microbiology
  • Interchangeable five (5) types of autoclavable glass vessel
  • Control up to sixteen (16) systems from a single interface
  • Compatible with microbial and cell culture applications
  • Intuitive and user-friendly control software in multi-language
  • Small pilot scale 15L and 20L glass vessel compatible
  • Ethernet communication with proSET SCADA software, and IP addressing
  • proSET EZScript software for advanced fermentation process (optional)


SKU: CSFS-07 Category:
Special Requirements?


proSET Evo System is the newest fermentor that offers an all-in-one fermentation solution and ultimate automation experience to ensure easy, smooth and productive bio-processing. The controller is pre-configured to support both thermostat and dry heating; together with its full compatibility to vessels from 0.5 to 20L, it provides a broad coverage to cultivation of most cell lines.

proSET Evo System is equipped with the latest control software; this user-friendly, intuitive software incorporates with many advanced functions to boost your experiment efficiency and thus lead to the success. Besides the manual controls of agitation, temperature, pH, DO level and feeding, one can also execute 15-step scheduled sequence controls on above parameters as well as pH and DO feedback controls. Moreover, a broad selection of plug-and-play optional devices are available to meet your specific requirement.

 PC software connects up to 16 systems & 16 vessels at the same time.

This unit has a 12 month limited warranty, for more information, consult our terms and conditions.

Control unit  Control panel  10.4” color touch-screen Interface (Resolution: 800 x 600 pixels)
 Communication port  Remote control through Ethernet
 Data export through USB port
 Analog AUX port for system extension
Number of vessels controlled per remote control software  Max. 16
 Program storage  Up to 59,994 programs for different kinds of condition
 Log data storage  Up to 100 process monitoring data files
 Cabinet material  ABS front panel and painted iron housing
 Dimension  Footprint: W x D = 15.75" x 22.44" (400mm x 570mm); Height: 29.14" (740mm)
 Rated voltage  110V~/220V~; 50/60 Hz, 10A
Aeration  Inlet gas flow-meter  0, 0.1-1LPM (0.5L); 0, 0.4 - 5LPM (1L); 0, 1 - 10LPM (3, 5L);  0, 2 - 20LPM (10L); 0, 4 – 50LPM (15, 20L)
 Sparger  Orifice ring
 Baffle  Removable 316L stainless steel baffles
Temperature Heating  1. Thermostat System: built-in heat exchanger  (550W heater, water circulation pump)
 2. Dry heating system (heating blanket or heating base unit)
Cooling  Cooling coil valve
 Range  with FS-V-A / B / C series : 5°C (41°F) above coolant up to 60°C (140°F);  with FS-V-D series :5°C(41°F) above coolant up to 90°C  (194°F);
 Probe  Platinum RTD probe (PT-100), non autoclavable
 Control mode  Manual or Programmable 15 steps PID control
Agitation  Drive  Removable top brushless motor (M3 for 0.5L, 1L; M2 for 3~20L)
 Speed range  a. For extremely shear-sensitive cell line: 30-300rpm  b. For fermentation and cell culture: 30 - 1200rpm (0.5-5L); 30 - 1000rpm (10L);      30 – 700rpm(15, 20L)
 Resolution  1rpm
 Impeller  2 impellers for 0.5L &1L vessel and double jacketed vessels   3 impellers for 3L vessel and above   Note: Customized impellers are available upon ordering
 Control mode  Manual or Programmable 15 steps PID control
pH  Range  2 - 14pH
 Resolution  0.01pH
 Probe  Gel-filled electrode, autoclavable
 Control mode  Manual/programmable 15 steps PID control with adjustable deadband,  pH stat with smart feeding technology
DO    Range  0 - 200%
 Resolution  0.10%
 Probe  Polarographic DO sensor; autoclavable
 Control mode    2-stage DO cascade response  a. Increase or decrease agitation speed   b. Oxygen Enrichment module(Optional)  c. Gas Mixing Station module(Optional)  DO stat with smart feeding technology
ORP(optional)  Measurement range  -/ 2000mV
 Resolution  1mV
 Probe  Gel-filled electrode: autoclavable    
Foam / Level  Probe  316L stainless steel protector with insulated PTFE tube, autoclavable, adjustable sensitivity control
Control Mode  Foam: On/Off switch  Level: On/Off switch control with wet/dry probe set up
Peristaltic Pump Pump number  4 x built-in pumps, 1 to 2 external pumps (Optional)
 Motor type  Precise stepping motor; minimum speed is 1rpm
 Speed range  0 to 65rpm
 Resolution  1rpm
 Control mode  Manual or Programmable 15 steps feeding control; pump can be assigned  for Acid, Base,        Antifoam and/or Substrate; pump can calculate flow rate and total volume
Exhaust Device type  316L stainless steel condenser
System Specification
Controller Duo heating system controller
Built-in rotameter
4 x built-in pump heads
Vessel Double jacketed vessel (includes glass body, head plate, T-handling bar, 2 probe adaptors) Single wall dish bottom vessel (includes glass body, head plate, T-handling bar, 2 probe adaptors) Airlifter vessel (includes glass body, head plate, draft tube, T-handling bar, 2 probe adaptors) Single wall dish bottom with heating blanket (includes glass body, head plate, T-handling bar, 2 probe adaptors and heating blanket) Single wall plain bottom with heating base unit (includes glass body, head plate, T-handling bar, 2 probe adaptors and heating base unit)
2 adjustable Pitched-blade impeller 3 adjustable Rushton-type impellers no impellers 3 adjustable Rushton-type impellers 3 adjustable Rushton-type impellers
Baffle assembled Draft tube assembled Baffle assembled
Condenser assembled
Air sparger assembled Micro sparger assembled Air sparger assembled
Motor Agitation motor N / A Agitation motor
Probes 1x pH probe and 1x pH cable
1x Do probe and 1x DO cable
1x Temperature probe and 1x Temperature cable
1x anti-foam/level sensor and 1x anti-foam/level sensor cable
Start-up Kit Complete start-up kit includes, silicone tubes, tube clamps, metal connector and autoclavable disc filters
Vessel Specification
Vessel Double Jacketed (FS-V-A series) Single Wall (FS-V-B series) Air Lifter (FS-V-C series)
Working volume 500ml 1L 3 L 5 L 10 L 1L 3 L 5 L 10 L 5 L
Total volume 1L 1.5L 3.8 L 6.8 L 12.5 L 1.5 L 3.8 L 6.8 L 12.5 L 7 L
Vessel Single Wall with Heating Blanket (FS-V-B series) Single Wall with Heating Base Unit (FS-V-D series)
Working volume 1L 3 L 5 L 10 L 15L 20L 3 L 5 L 10 L
Total volume 1.5L 3.8 L 6.8 L 12.5 L 18L 24.4L 3.7 L 6.4 L 13.4L
Utility Requirement
Power source 100-120V / 210-230V, 50-60Hz with electrical safety cutoff switch
Water source 10 psig (0.69 barg), 14.5 psig (1 barg) maximum; (water supplied to fermenter must be softed and at least 15°C below set operating temperature)
Air source 10 psig (0.69 barg), 29.0 psig (2 barg) maximum; air must be dry, oil-free and filtered
Sterilization Autoclave; size of the autoclave inner chamber must be able to accomodate vessel with condenser attached

This product has been cited in the following research papers:

Reference title Date Citation Product usage
Biosynthesis of chitosan-Oligosaccharides (COS) by non-aflatoxigenic Aspergillus sp. strain EGY1 DSM 101520: A robust biotechnological approach 2018 Amira M. Embaby, Ramy R. Melika, Ahmed Hussein, Amal H. El-Kamel, Heba S.Marey, Process Biochemistry, Volume 64, January 2018, Pages 16-30, …Fig. 4. Batch cultivation of Aspergillus sp. section Flavi strain EGY1 DSM 101520 in a laboratory scale fermenter. Production medium (4 liters) with an initial pH of 5.0 in a 7-liters Cleaver laboratory scale fermenter was inoculated with 5% (v/v) seed culture previously grown for 72 h at 30 °C. The process was conducted at 25 °C under automated controlled pH. Each data point is expressed as the a…
Scaling-Up of Natural Products Isolation 2012 Cite this protocol as: Kumarasamy Y. (2012) Scaling-Up of Natural Products Isolation. In: Sarker S., Nahar L. (eds) Natural Products Isolation. Methods in Molecular Biology (Methods and Protocols), vol 864. Humana Press … useful feature of this system is that it is available with interchangeable vessels which range from 2.2 L to 14.0 L. Other companies that produce similar fermentors include Minifors®, Lavalabs®, and Cleaver Scientific. Bench-top fermenters are usually cheaper to purchase than the trolley mounted or skid-mounted fermenters. The entry level fermentors are simple in construction and can be thought o…