omniBLOT Blotter

£447.00£719.00

Key Features

  • Mini System transfers up to 4 Mini gels
  • Maxi system trasnfers up to 4 Maxi gels, 8 Mini Wide and 16 Mini gels
  • Complete run-time flexibility from overnight to as short as a couple of hours
  • Cool pack for environmentally friendly low-cost cooling during high-intensity transfers
SKU: SBLOT Category:
Special Requirements?

Description

The Mini omniPAGE Sub Blot systems are complete stand-alone units for submarine blotting. Each unit comprises an omniPAGE tank and lid, electroblotting insert, and cassettes and fibre pads. The versatile design of the omniPAGE tank and lid allows the unit to be easily adapted for vertical PAGE or IEF with capillary tube gels, using the relevant optional insert. Other features include:


  • Multi-transfer capability for 4 Mini gels in the Mini system

  • Complete run-time flexibility from overnight to as short as a couple of hours

  • Cool pack for environmentally friendly low-cost cooling during high-intensity transfers

  • Open design, rigid cassettes maximise current transfer and eliminate shadow band formation

  • Cassettes colour-coded prevent polarity reversal to ensure transfer in the correct orientation



The Maxi format omniPAGE Sub Blot system is a complete stand-alone unit for submarine blotting. Each unit comprises an omniPAGE tank and lid, electroblotting insert, and cassettes and fibre pads. The versatile design of the omniPAGE tank and lid allows the unit to be easily adapted for vertical PAGE or IEF with capillary tube gels, using the relevant optional insert. Other features include:


  • Multi-transfer capability for up to 4 Maxi gels, 8 Mini Wide and 16 Mini gels

  • Complete run-time flexibility from overnight to as short as a couple of hours

  • Cool pack for environmentally friendly low-cost cooling during high-intensity transfers

  • Open design, rigid cassettes maximise current transfer and eliminate shadow band formation

  • Cassettes colour-coded prevent polarity reversal to ensure transfer in the correct orientation


Catalogue Page Number: 36

SKUSB10SB10WSB20
Unit Dimensions (cm)19 x 13 x 1526 x 16 x 1630 x 18 x 27
Capacity4 Gels4 Gels4 Gels
Typical Running Conditions100V, 1-2 hr100V, 1-2 hr100V, 5-20h
Gel Dimensions8 x 8.516 x 8.516 x 17.5
Maximum Buffer Volume (ml)120028006400
Blotter TypeWet BlotWet BlotWet Blot

This product has been cited in the following research papers:

Reference title Date Citation Product usage
Amidase activity is essential for medial localization of AmiC in Caulobacter crescentus 2018 Dubey, A. & Priyadarshini, R. Curr Genet (2018) 64: 661. https://doi.org/10.1007/s00294-017-0781-9 …Cell lysates were analyzed by SDS–PAGE and western blot. After SDS–PAGE gels were transferred to a polyvinylidene difluoride membrane using a wet transfer apparatus (Cleaver Scientific). After overnight incubation with 5% nonfat milk in PBS at 4 °C, the membranes were washed once with PBST and incubated with antibodies against m-Cherry (Thermo Scientific) with dilution ratio (1:2000) for 1 h at ro…
Restorative effect of l-Dopa treatment against Ochratoxin A induced neurotoxicity 2018 Pratiksha V. Bhat, T. Anand, T. Mohan Manu, Farhath Khanum, Neurochemistry International, In press, corrected proof, Available online 5 April 2018, …tein levels were estimated by Lowry’s method (1951). 150 μg of protein homogenates were separated on SDS-PAGE followed by transferring onto a nitrocellulose membrane by an electro blotting apparatus (cleaver scientific Ltd, UK). After transferring the membranes were probed with monoclonal antibodies β-actin (1:1000), Akt (1:1000), phospho-Akt (1:1000) and FoxO3a (1:1000) followed by incubation at …
Attenuation of cytotoxicity induced by tBHP in H9C2 cells by Bacopa monniera and Bacoside A 2018 Mohan Manu T, T. Anand, Farhath Khanum, Pathophysiology, Volume 25, Issue 2, June 2018, Pages 143-149, …cation by Lowry’s method (1951). Protein sample of 50 μg was loaded to each well to separate total cellular proteins and transferred onto a nitrocellulose membrane using an electroblotting apparatus (cleaver scientific Ltd, UK). Proteins transferred onto membranes were blocked with 5% milk in TBST for overnight at 4 °C. Membranes were probed with primary antibodies of GAPDH (sc-5286), Bcl2 (sc-863…
Patulin Induced Oxidative Stress Mediated Apoptotic Damage in Mice, and its Modulation by Green Tea Leaves 2017 Girindrababu Venkattappa Jayashree, Krishnaswamy Krupashree, Puttasitdiah Rachitha, Farhath Khanum, Journal of Clinical and Experimental Hepatology, Volume 7, Issue 2, June 2017, Pages 127-134, …in contents were determined as mentioned earlier. Samples were (50 μg of proteins) separated by 10–15% SDS-PAGE followed by transferred to nitrocellulose membrane using an electro blotting apparatus (cleaver scientific Ltd, UK). The membranes were then blocked overnight at 4 °C with 5% (v/v) non-fat dry milk in Tris-buffered saline with Tween-20 (TBS-T) (10 mM Tris–HCl, 150 mM NaCl, and 0.1% Tween…
Cytoprotective propensity of Bacopa monniera against hydrogen peroxide induced oxidative damage in neuronal and lung epithelial cells 2016 Pandareesh, M.D., Anand, T. & Bhat, P.V. Cytotechnology (2016) 68: 157. https://doi.org/10.1007/s10616-014-9767-3 …Total cellular protein was separated on SDS-PAGE and transferred onto a nitrocellulose membrane using an electro blotting apparatus (Cleaver Scientific Ltd, UK) as per earlier method (Anand et al. 2012). After transfer, the membranes were probed with α-Tubulin (sc-5286), SOD (sc-8637), α-CAT (sc-34280), α-GPx (sc-22146) and α-GR (sc-32408) (Santa Cruz Biotechnology, Santa Cruz, CA, USA) at 1: 1,00…
Cognition Enhancing and Neuromodulatory Propensity of Bacopa monniera Extract Against Scopolamine Induced Cognitive Impairments in Rat Hippocampus 2016 Pandareesh, M.D., Anand, T. & Khanum, F. Neurochem Res (2016) 41: 985. https://doi.org/10.1007/s11064-015-1780-1 …red by the method of Lowry et al. [57]. Tissue homogenates containing 150 μg of proteins were separated on SDS-PAGE and transferred onto a nitrocellulose membrane using an electro blotting apparatus (Cleaver Scientific Ltd, UK). After transfer, the membranes were probed with GAPDH (sc-25778) (Santa Cruz Biotechnology, CA, USA) at 1: 1000 dilutions and monoclonal anti-CREB (C8977, Sigma, St.Louis, …
The Level of ALR is Regulated by the Quantity of Mitochondrial DNA 2016 Balogh, T., Lőrincz, T., Stiller, I. et al. Pathol. Oncol. Res. (2016) 22: 431. https://doi.org/10.1007/s12253-015-0020-y … to the manufacturer’s protocol. 50 μg amounts of total protein samples were loaded onto 15 % polyacrylamide gel under reducing conditions and transferred to a 0.45 μm nitrocellulose membrane using a Cleaver electrophoresis and Western-blot apparatus (Cleaver Scientific, Warwickshire, UK). After Ponceau staining the membranes were blocked with Saturating-solution (5 % non-fat dry milk in TBS-Tween…
Attenuation of smoke induced neuronal and physiological changes by bacoside rich extract in Wistar rats via down regulation of HO-1 and iNOS 2014 M. D. Pandareesh, T. Anand, NeuroToxicology, Volume 40, January 2014, Pages 33-42, …ed by the method of Lowry et al. (1951). Tissue homogenates containing 50 μg of proteins were separated on SDS-PAGE and transferred onto a nitrocellulose membrane using an electro blotting apparatus (cleaver scientific Ltd, UK). After transfer, the membranes were probed with primary antibodies against α-Tubulin B-7 (sc-5286) and HSP-70 C92F3A (sc-66048) (Santa Cruz Biotechnology, CA, USA) at 1: 10…
Non structural protein of avian influenza A (H11N1) virus is a weaker suppressor of immune responses but capable of inducing apoptosis in host cells 2012 Mukherjee, S., Majumdar, S., Vipat, V.C. et al. Virol J (2012) 9: 149. https://doi.org/10.1186/1743-422X-9-149 …0 μg) from cell extracts were separated by 12.5% SDS-polyacrylamide gel electrophoresis (12.5% SDS-PAGE) and transferred to Hybond-C (Amersham Biosciences) membrane with an electrotransfer apparatus (Cleaver Scientific Ltd) at 10 Volts (100 mA) for 1 h. The membrane was probed with specific monoclonal antibodies against Influenza A-NS1 protein (Santa Cruz Biotechnology, Inc. Santa Cruz, CA). Prima…
Pandemic (H1N1) 2009 influenza virus induces weaker host immune responses in vitro: a possible mechanism of high transmissibility 2011 Mukherjee, S., Vipat, V.C., Mishra, A.C. et al. Virol J (2011) 8: 140. https://doi.org/10.1186/1743-422X-8-140 …0 μg) from cell extracts were separated by 12.5% SDS-polyacrylamide gel electrophoresis (12.5% SDS-PAGE) and transferred to Hybond-C (Amersham Biosciences) membrane with an electrotransfer apparatus (Cleaver Scientific Ltd) at 10 Volts (100 mA) for 1 h 30 min. Primary and secondary antibody interaction was performed in phosphate-buffered saline (pH 7.5). Primary antibodies used were rabbit anti-ST…

Accessories…

omniBLOT Mini Insert
VS10BI £290.00
omniBLOT Mini Cassette (Including 2 foam Pads)
SB10C £30.00
omniBLOT Mini Fibre pads – Pack of 8
SB10F £30.00
omniBLOT Maxi Insert
VS20BI £420.00
omniBLOT Maxi Cassette (including 2 foam pads)
SB20C £50.00
omniBLOT Maxi Fibre pads – Pack of 6
SB20F £50.00
White General Pupose Spill Trays
CSR-TW £35.00
White BioHazard Spill Trays
CSR-TO £37.00
Spill Tray Liners
CSR-TL £43.00
10cm acrylic roller for western blotting
CSL-WBROLLER £25.00