| Reference title | Date | Citation | Product usage |
|---|
| Amidase activity is essential for medial localization of AmiC in Caulobacter crescentus | 2018 | Dubey, A. & Priyadarshini, R. Curr Genet (2018) 64: 661. https://doi.org/10.1007/s00294-017-0781-9 | …Cell lysates were analyzed by SDS–PAGE and western blot. After SDS–PAGE gels were transferred to a polyvinylidene difluoride membrane using a wet transfer apparatus (Cleaver Scientific). After overnight incubation with 5% nonfat milk in PBS at 4 °C, the membranes were washed once with PBST and incubated with antibodies against m-Cherry (Thermo Scientific) with dilution ratio (1:2000) for 1 h at ro… |
| Restorative effect of l-Dopa treatment against Ochratoxin A induced neurotoxicity | 2018 | Pratiksha V. Bhat, T. Anand, T. Mohan Manu, Farhath Khanum, Neurochemistry International, In press, corrected proof, Available online 5 April 2018, | …tein levels were estimated by Lowry’s method (1951). 150 μg of protein homogenates were separated on SDS-PAGE followed by transferring onto a nitrocellulose membrane by an electro blotting apparatus (cleaver scientific Ltd, UK). After transferring the membranes were probed with monoclonal antibodies β-actin (1:1000), Akt (1:1000), phospho-Akt (1:1000) and FoxO3a (1:1000) followed by incubation at … |
| Attenuation of cytotoxicity induced by tBHP in H9C2 cells by Bacopa monniera and Bacoside A | 2018 | Mohan Manu T, T. Anand, Farhath Khanum, Pathophysiology, Volume 25, Issue 2, June 2018, Pages 143-149, | …cation by Lowry’s method (1951). Protein sample of 50 μg was loaded to each well to separate total cellular proteins and transferred onto a nitrocellulose membrane using an electroblotting apparatus (cleaver scientific Ltd, UK). Proteins transferred onto membranes were blocked with 5% milk in TBST for overnight at 4 °C. Membranes were probed with primary antibodies of GAPDH (sc-5286), Bcl2 (sc-863… |
| Patulin Induced Oxidative Stress Mediated Apoptotic Damage in Mice, and its Modulation by Green Tea Leaves | 2017 | Girindrababu Venkattappa Jayashree, Krishnaswamy Krupashree, Puttasitdiah Rachitha, Farhath Khanum, Journal of Clinical and Experimental Hepatology, Volume 7, Issue 2, June 2017, Pages 127-134, | …in contents were determined as mentioned earlier. Samples were (50 μg of proteins) separated by 10–15% SDS-PAGE followed by transferred to nitrocellulose membrane using an electro blotting apparatus (cleaver scientific Ltd, UK). The membranes were then blocked overnight at 4 °C with 5% (v/v) non-fat dry milk in Tris-buffered saline with Tween-20 (TBS-T) (10 mM Tris–HCl, 150 mM NaCl, and 0.1% Tween… |
| Cytoprotective propensity of Bacopa monniera against hydrogen peroxide induced oxidative damage in neuronal and lung epithelial cells | 2016 | Pandareesh, M.D., Anand, T. & Bhat, P.V. Cytotechnology (2016) 68: 157. https://doi.org/10.1007/s10616-014-9767-3 | …Total cellular protein was separated on SDS-PAGE and transferred onto a nitrocellulose membrane using an electro blotting apparatus (Cleaver Scientific Ltd, UK) as per earlier method (Anand et al. 2012). After transfer, the membranes were probed with α-Tubulin (sc-5286), SOD (sc-8637), α-CAT (sc-34280), α-GPx (sc-22146) and α-GR (sc-32408) (Santa Cruz Biotechnology, Santa Cruz, CA, USA) at 1: 1,00… |
| Cognition Enhancing and Neuromodulatory Propensity of Bacopa monniera Extract Against Scopolamine Induced Cognitive Impairments in Rat Hippocampus | 2016 | Pandareesh, M.D., Anand, T. & Khanum, F. Neurochem Res (2016) 41: 985. https://doi.org/10.1007/s11064-015-1780-1 | …red by the method of Lowry et al. [57]. Tissue homogenates containing 150 μg of proteins were separated on SDS-PAGE and transferred onto a nitrocellulose membrane using an electro blotting apparatus (Cleaver Scientific Ltd, UK). After transfer, the membranes were probed with GAPDH (sc-25778) (Santa Cruz Biotechnology, CA, USA) at 1: 1000 dilutions and monoclonal anti-CREB (C8977, Sigma, St.Louis, … |
| The Level of ALR is Regulated by the Quantity of Mitochondrial DNA | 2016 | Balogh, T., Lőrincz, T., Stiller, I. et al. Pathol. Oncol. Res. (2016) 22: 431. https://doi.org/10.1007/s12253-015-0020-y | … to the manufacturer’s protocol. 50 μg amounts of total protein samples were loaded onto 15 % polyacrylamide gel under reducing conditions and transferred to a 0.45 μm nitrocellulose membrane using a Cleaver electrophoresis and Western-blot apparatus (Cleaver Scientific, Warwickshire, UK). After Ponceau staining the membranes were blocked with Saturating-solution (5 % non-fat dry milk in TBS-Tween… |
| Attenuation of smoke induced neuronal and physiological changes by bacoside rich extract in Wistar rats via down regulation of HO-1 and iNOS | 2014 | M. D. Pandareesh, T. Anand, NeuroToxicology, Volume 40, January 2014, Pages 33-42, | …ed by the method of Lowry et al. (1951). Tissue homogenates containing 50 μg of proteins were separated on SDS-PAGE and transferred onto a nitrocellulose membrane using an electro blotting apparatus (cleaver scientific Ltd, UK). After transfer, the membranes were probed with primary antibodies against α-Tubulin B-7 (sc-5286) and HSP-70 C92F3A (sc-66048) (Santa Cruz Biotechnology, CA, USA) at 1: 10… |
| Non structural protein of avian influenza A (H11N1) virus is a weaker suppressor of immune responses but capable of inducing apoptosis in host cells | 2012 | Mukherjee, S., Majumdar, S., Vipat, V.C. et al. Virol J (2012) 9: 149. https://doi.org/10.1186/1743-422X-9-149 | …0 μg) from cell extracts were separated by 12.5% SDS-polyacrylamide gel electrophoresis (12.5% SDS-PAGE) and transferred to Hybond-C (Amersham Biosciences) membrane with an electrotransfer apparatus (Cleaver Scientific Ltd) at 10 Volts (100 mA) for 1 h. The membrane was probed with specific monoclonal antibodies against Influenza A-NS1 protein (Santa Cruz Biotechnology, Inc. Santa Cruz, CA). Prima… |
| Pandemic (H1N1) 2009 influenza virus induces weaker host immune responses in vitro: a possible mechanism of high transmissibility | 2011 | Mukherjee, S., Vipat, V.C., Mishra, A.C. et al. Virol J (2011) 8: 140. https://doi.org/10.1186/1743-422X-8-140 | …0 μg) from cell extracts were separated by 12.5% SDS-polyacrylamide gel electrophoresis (12.5% SDS-PAGE) and transferred to Hybond-C (Amersham Biosciences) membrane with an electrotransfer apparatus (Cleaver Scientific Ltd) at 10 Volts (100 mA) for 1 h 30 min. Primary and secondary antibody interaction was performed in phosphate-buffered saline (pH 7.5). Primary antibodies used were rabbit anti-ST… |
