| Reference title | Date | Citation | Product usage |
|---|
| A combinatorial approach for robust transgene delivery and targeted expression in mammary gland for generating biotherapeutics in milk, bypassing germline gene integration | 2018 | Ganguli, N., Ganguli, N., Chandra, S. et al. Appl Microbiol Biotechnol (2018) 102: 6221. https://doi.org/10.1007/s00253-018-9094-2 | … Loading volume for each sample was 15 μl along with dye. DNA was denatured at 95 °C for 10 min prior blotting on to Hybond N+ (Amersham Pharmacia Biotech, England) membrane in a slot blot apparatus (Cleaver Scientific Co., England) under vacuum. The membrane was UV cross-linked (Church and Gilbert 1984) at 12 × 104 μJ/cm2 energy in a CL-1000 Ultraviolet crosslinker (UVP, Upland, CA, USA). The cro… |
| Modulation of immune response and protective efficacy of recombinant outer-membrane protein F (rOmpF) of Aeromonas hydrophila in Labeo rohita | 2018 | Sunita Kumari Yadav, Pujarini Dash, Pramoda Kumar Sahoo, Lalit C. Garg, Aparna Dixit, Fish & Shellfish Immunology, In press, accepted manuscript, Available online 27 June 2018, | …e study [12] together with other bacteria/proteins was performed. For this, the cell lysates (1 μg each) of various Aeromonas strains were blotted onto a nitrocellulose membrane using a slot blotter (cleaver scientific Ltd, UK) and developed in a manner similar to western blot assay.… |
| Recombinant outer membrane protein C of Aeromonas hydrophila elicits mixed immune response and generates agglutinating antibodies | 2016 | Yadav, S.K., Meena, J.K., Sharma, M. et al. Immunol Res (2016) 64: 1087. https://doi.org/10.1007/s12026-016-8807-9 | … cells and Chinese hamster ovary cells (CHO-K1) and BSA were included as negative controls. Cell lysates (1 µg each) of various strains slot blotted onto a nitrocellulose (NC) membrane (Slot blotter, Cleaver Scientific Ltd, UK) were subjected to Western blot analysis using anti-rOmpC antisera. After blocking the non-specific sites with BSA (2 % in 1× PBS) followed by 3 washes of 10 min each with w… |
| Robust generation of transgenic mice by simple hypotonic solution mediated delivery of transgene in testicular germ cells | 2016 | Abul Usmani, Nirmalya Ganguli, Subodh K Jain, Nilanjana Ganguli, Subeer S Majumdar, Molecular Therapy – Methods & Clinical Development, Volume 3, 2016, Article 16076, | …P using High Prime DNA labeling kit (Roche Diagnostic GmbH, Mannheim, Germany). Denatured gDNA (1 μg) was blotted on Hybond N+ (Amersham Pharmacia Biotech, England) membrane with slot blot apparatus (cleaver scientific, Warwickshire, UK) under vacuum. The membranes were prehybridized for 4 hours, followed by hybridization with respective transgene specific probes for 10–12 hours. The hybridized bl… |
| Characterization of immune response elicited by the recombinant outer membrane protein OmpF of Aeromonas hydrophila, a potential vaccine candidate in murine model | 2014 | Yadav, S.K., Sahoo, P.K. & Dixit, A. Mol Biol Rep (2014) 41: 1837. https://doi.org/10.1007/s11033-014-3033-9 | …nas isolates together with other bacteria/proteins was performed. For this, the cell lysates (1 μg each) of various Aeromonas strains were blotted onto a nitrocellulose membrane using a slot blotter (Cleaver Scientific Ltd, UK). BSA (2 %) made in 1 × PBST was used for blocking the non-specific sites for 1 h at 37 °C. The membrane was washed thrice with washing buffer (0.15 M PBS, pH 7.3, and 0.2 %… |
| Comparison of the Anti-inflammatory and Anti-nociceptive Effects of Cortistatin-14 and Somatostatin-14 in Distinct In Vitro and In Vivo Model Systems | 2012 | Markovics, A., Szőke, É., Sándor, K. et al. J Mol Neurosci (2012) 46: 40. https://doi.org/10.1007/s12031-011-9577-4 | …specific binding was subtracted from total binding. The reaction was terminated by filtrating the samples through Whatman GF/B glass fiber filters (Whatman, UK). Using 48-well Slot Blot Manifold from Cleaver Scientific filters were washed three times with ice-cold 50 mM Tris–HCl buffer (pH 7.4). After drying for 60 min at 37°C, radioactivity was measured in scintillation liquid in a Packard Tri-Ca… |
