dotBLOT and slotBLOT


Key Features

  • Low cost
  • Simple construction
  • Easy assembly
  • Four sample configurations
  • Alpha-numeric sample identification
SKU: DOTSLOT Category:
Special Requirements?


Four different sample number, size and style of Hybridisation Manifold are offered; two types of Dot Blotter and two types of Slot Blotter.

Typical applications include clone screening with DNA/RNA probes in Southern/Northern blots and immunological screening with antibodies in Western blots. The units incorporate precision lapped surfaces to ensure uniform blotting membrane contact and a leak proof gasket. These prevent lateral transfer of samples and smudging by ensuring that a complete vacuum is formed. Six thumbscrews ensure even and tight sealing for fast sample suck down.

Dot Blotters are available in 48 and 96 well versions and Slot Blotters in 24 and 48 well.

Catalogue Page Number: 45

Blotter Type Vacuum/Capillary Vacuum/Capillary Vacuum/Capillary Vacuum/Capillary
Capacity 48 Samples, 3 x 16 96 Samples, 8 x 12 24 Samples, 2 x 12 48 Samples, 3 x 16
Size 12.1 x 4.4 cm membrane 11 x 7.4 cm membrane 12.1 x 4.4 cm  membrane 12.1 x 4.4 cm membrane
Unit Dimensions (cm) 6 x 9.5 x 10 cm 6 x 10.5 x 14 6 x 7.4 x 8.3 6 x 9.5 x 10
Vacuum Pump Type 600mg Hg 0.8 BAR with cold trap

This product has been cited in the following research papers:

Reference title Date Citation Product usage
A combinatorial approach for robust transgene delivery and targeted expression in mammary gland for generating biotherapeutics in milk, bypassing germline gene integration 2018 Ganguli, N., Ganguli, N., Chandra, S. et al. Appl Microbiol Biotechnol (2018) 102: 6221. … Loading volume for each sample was 15 μl along with dye. DNA was denatured at 95 °C for 10 min prior blotting on to Hybond N+ (Amersham Pharmacia Biotech, England) membrane in a slot blot apparatus (Cleaver Scientific Co., England) under vacuum. The membrane was UV cross-linked (Church and Gilbert 1984) at 12 × 104 μJ/cm2 energy in a CL-1000 Ultraviolet crosslinker (UVP, Upland, CA, USA). The cro…
Modulation of immune response and protective efficacy of recombinant outer-membrane protein F (rOmpF) of Aeromonas hydrophila in Labeo rohita 2018 Sunita Kumari Yadav, Pujarini Dash, Pramoda Kumar Sahoo, Lalit C. Garg, Aparna Dixit, Fish & Shellfish Immunology, In press, accepted manuscript, Available online 27 June 2018, …e study [12] together with other bacteria/proteins was performed. For this, the cell lysates (1 μg each) of various Aeromonas strains were blotted onto a nitrocellulose membrane using a slot blotter (cleaver scientific Ltd, UK) and developed in a manner similar to western blot assay.…
Recombinant outer membrane protein C of Aeromonas hydrophila elicits mixed immune response and generates agglutinating antibodies 2016 Yadav, S.K., Meena, J.K., Sharma, M. et al. Immunol Res (2016) 64: 1087. … cells and Chinese hamster ovary cells (CHO-K1) and BSA were included as negative controls. Cell lysates (1 µg each) of various strains slot blotted onto a nitrocellulose (NC) membrane (Slot blotter, Cleaver Scientific Ltd, UK) were subjected to Western blot analysis using anti-rOmpC antisera. After blocking the non-specific sites with BSA (2 % in 1× PBS) followed by 3 washes of 10 min each with w…
Robust generation of transgenic mice by simple hypotonic solution mediated delivery of transgene in testicular germ cells 2016 Abul Usmani, Nirmalya Ganguli, Subodh K Jain, Nilanjana Ganguli, Subeer S Majumdar, Molecular Therapy – Methods & Clinical Development, Volume 3, 2016, Article 16076, …P using High Prime DNA labeling kit (Roche Diagnostic GmbH, Mannheim, Germany). Denatured gDNA (1 μg) was blotted on Hybond N+ (Amersham Pharmacia Biotech, England) membrane with slot blot apparatus (cleaver scientific, Warwickshire, UK) under vacuum. The membranes were prehybridized for 4 hours, followed by hybridization with respective transgene specific probes for 10–12 hours. The hybridized bl…
Characterization of immune response elicited by the recombinant outer membrane protein OmpF of Aeromonas hydrophila, a potential vaccine candidate in murine model 2014 Yadav, S.K., Sahoo, P.K. & Dixit, A. Mol Biol Rep (2014) 41: 1837. …nas isolates together with other bacteria/proteins was performed. For this, the cell lysates (1 μg each) of various Aeromonas strains were blotted onto a nitrocellulose membrane using a slot blotter (Cleaver Scientific Ltd, UK). BSA (2 %) made in 1 × PBST was used for blocking the non-specific sites for 1 h at 37 °C. The membrane was washed thrice with washing buffer (0.15 M PBS, pH 7.3, and 0.2 %…
Comparison of the Anti-inflammatory and Anti-nociceptive Effects of Cortistatin-14 and Somatostatin-14 in Distinct In Vitro and In Vivo Model Systems 2012 Markovics, A., Szőke, É., Sándor, K. et al. J Mol Neurosci (2012) 46: 40. …specific binding was subtracted from total binding. The reaction was terminated by filtrating the samples through Whatman GF/B glass fiber filters (Whatman, UK). Using 48-well Slot Blot Manifold from Cleaver Scientific filters were washed three times with ice-cold 50 mM Tris–HCl buffer (pH 7.4). After drying for 60 min at 37°C, radioactivity was measured in scintillation liquid in a Packard Tri-Ca…