Comet Assay Tanks for 10 – 40 Slides

£368.00£638.00

Key Features

  • For Single Cell Gel Electrophoresis
  • Minimise exposure to light and reduce background DNA damage
  • High efficiency cooling for enhanced resolution
SKU: CSL-COM Category:
Special Requirements?

Description

Cleaver Scientific Comet Assay Tanks are available in four slide formats to study single cell gel electrophoresis (SCGE), a technique made popular by drug toxicology and carcinogenesis studies for the detection and quantifation of DNA damage in cells. Each tank’s robust construction from ebony acrylic ensures that cells remain free of exposure to background light and DNA damage during electrophoresis, while a cooled central platform provides a convenient surface for slide preparation and control of slide temperature during the assay. Following electrophoresis DNA damage may be measured using Comet Assay scoring software.

Background: First introduced in 1981 to quantify double-stranded DNA breakages in single cells exposed to irradiation, the Comet Assay (or SCGE) has since been adapted to analyse specific DNA lesions and repair processes.

Overview: Following genotoxic insult, such as ionizing radiation, the resultant strand breakage of supercoiled duplex DNA reduces the size of the large genomic DNA from which these strands are separated or drawn out by electrophoresis. The genomic DNA then takes on the appearance of a comet as its negatively charged broken ends and fragments migrate towards the anode during electrophoresis.

Method: After exposure to a genotoxic insult cells are suspended within low melting point agarose and embedded within a thin layer of agarose on a microscope slide. Cellular protein is then removed by lysis in detergent, when DNA is allowed to unwind in alkaline conditions before electrophoresis. The DNA is electrophoresed, stained and then analysed using software.

Results: Microscopy imaging is used to measure DNA fluorescence upon staining. In DNA damaged cells the resultant image resembles a ‘comet’ with the cellular DNA separated into a head and tail. The head is mainly composed of intact genomic DNA, whereas any fragmented or damaged DNA is concentrated within and towards the tail.

Catalogue Page Number: 50

SKUCSL-COM10CSL-COM20CSL-COM40
Unit Dimensions (cm)17 x 34 x 931 x 34 x 933 x 59 x 9
Slide Capacity102040
Base Buffer Volume55010002100
Typical Running Conditions25V/300mA for 1h25V/300mA for 1h25V/300mA for 1h
Includes Casting BaseFALSEFALSEFALSE
Includes Additional CasterFALSEFALSEFALSE

This product has been cited in the following research papers:

Reference title Date Citation Product usage
Dose and Exposure Time-Dependent Renal and Hepatic Effects of Intraperitoneally Administered Fumonisin B1 in Rats 2018 Szabó, A., Szabó-Fodor, J., Kachlek, M., Mézes, M., Balogh, K., Glávits, R., Ali, O., Zeebone, Y. and Kovács, M., 2018. Dose and Exposure Time-Dependent Renal and Hepatic Effects of Intraperitoneally Administered Fumonisin B1 in Rats. Toxins, 10(11), p.465. … solution (1% Triton X-100, 2.5M NaCl, 10 mM Tris, 0.1 M EDTA, pH10) for 1 h at 4 °C. Following the lysis the slides were placed in alkaline electrophoresis buffer (pH 13) in an electrophoresis tank (Cleaver Scientific Ltd., Warwickshire, UK) for 40 min at 4 °C, followed by electrophoresis at 25 V (300 mA) for 30 min at 4 °C. The slides were then placed in neutralizing buffer (pH 7.5) and washed t…
Application of the comet assay, micronucleus test and global DNA methylation analysis in Darevskia lizards as a sentinel organism for genotoxic monitoring of soil pollution 2018 Sargsyan, A., Simonyan, A., Hovhannisyan, G., Arakelyan, M. and Aroutiounian, R., 2018. Application of the comet assay, micronucleus test and global DNA methylation analysis in Darevskia lizards as a sentinel organism for genotoxic monitoring of soil pollution. Mutation Research/Genetic Toxicology and Environmental Mutagenesis. …5 M NaCl, 100 mM Na2EDTA, 10 mM Tris and 1% Triton X-100, pH 10.0) for 24 h. The slides were then placed in alkaline buffer (300 mM NaOH and 1 mM Na2EDTA, pH > 13) for 20 min in electrophoresis tank (Cleaver Scientific, Rugby, UK) for DNA unwinding, and electrophoresed for 15 min at 300 mA and 1 V/cm in the same buffer. Slides were then neutralized with 0.4 M Tris, pH 7.5 for 10 min and stained wi…
Interaction between the three frequently co‐occurring Fusarium mycotoxins in rats 2018 Szabó‐Fodor, J., Szabó, A., Kócsó, D., Marosi, K., Bóta, B., Kachlek, M., Mézes, M., Balogh, K., Kövér, G., Nagy, I. and Glávits, R., 2018. Interaction between the three frequently co‐occurring Fusarium mycotoxins in rats. Journal of animal physiology and animal nutrition. …lution (1% Triton X‐100, 2.5 M NaCl, 10 mM Tris, 0.1 M EDTA, pH 10) for 1 hr at 4°C. Following the lysis, the slides were placed in alkaline electrophoresis buffer (pH 13) in an electrophoresis tank (Cleaver Scientific, Warwickshire, UK) for 40 min at 4°C, followed by electrophoresis at 25 V (300 mA) for 30 min at 4°C. The slides were then placed in neutralising buffer (pH 7.5) and washed three ti…
l‐arginine protects against oxidative damage induced by T‐2 toxin in mouse Leydig cells 2018 Yang, J.Y., Zhang, Y.F., Li, Y.X., Meng, X.P. and Bao, J.F., 2018. l‐arginine protects against oxidative damage induced by T‐2 toxin in mouse Leydig cells. Journal of biochemical and molecular toxicology, p.e22209. ….5 M NaCl, 10 mM Tris, 0.1 M ethylenediaminetetraacetic acid, pH 10) for 1 hour at 4°C. The resulting nucleic acids were unwound in alkaline electrophoresis buffer (pH 13) in an electrophoresis tank (Cleaver Scientific Ltd, Warwickshire, UK) for 40 minutes at 4°C and followed by electrophoresis at 25 V (300 mA) for 30 minutes at 4°C. The slides were placed in neutralizing buffer (pH 7.5) with thre…
Glibenclamide protects against thioacetamide-induced hepatic damage in Wistar rat: investigation on NLRP3, MMP-2, and stellate cell activation 2018 Dwivedi, D.K. and Jena, G.B., 2018. Glibenclamide protects against thioacetamide-induced hepatic damage in Wistar rat: investigation on NLRP3, MMP-2, and stellate cell activation. Naunyn-Schmiedeberg’s archives of pharmacology, pp.1-18. …ed water for the removal of detergents and salts. Slides were submerged in chilled running buffer [1 mM EDTA and 300 mM NaOH (pH 13)] for 20 min in horizontal electrophoresis tank [(model, CSLCOM20), Cleaver Scientific Ltd., Warwickshire, UK] for unwinding of DNA. Further, electrophoresis was performed at 300 mA and 38 V (0.90 V/cm) for 30 min. The slides were treated with neutralizing buffer cont…
Predator Odor Destabilizes the Cell Genome of the Mouse Bone Marrow 2018 Glinin, T.S., Starshova, P.A., Shubina, V.A. et al. Russ J Genet Appl Res (2018) 8: 101. https://doi.org/10.1134/S2079059718020041 …ns. Subsequently, each micropreparation was covered by a band of sealing film (Parafilm) and incubated for 1 h. Then the micropreparations were transferred into an electrophoresis chamber (CSL-COM10, Cleaver Scientific) cooled to 4°C with an electrophoretic buffer (300 mM NaOH, 1 mM EDTA-Na2 (pH > 13)) and incubated for 20 min, after which electrophoresis was conducted for 20 min at a field streng…
Fibroblasts potentiate melanoma cells in vitro invasiveness induced by UV-irradiated keratinocytes 2018 Jobe, N.P., Živicová, V., Mifková, A. et al. Histochem Cell Biol (2018) 149: 503. https://doi.org/10.1007/s00418-018-1650-4 …se and poured on negatively charged glass slides at density 20,000/slide. After the cell lysis and alkaline treatment (pH 13), the electrophoresis was performed in a light protected Comet Assay Tank (Cleaver Scientific, Rugby, United Kingdom) at 25 V for 30 min in the cold room at 4 °C. The nuclei were finally counterstained with 4′,6-diamidino-2-phenylindole and imaged as described above on fluor…
Assessment of neurohepatic DNA damage in male Sprague–Dawley rats exposed to organophosphates and pyrethroid insecticides 2018 Yahia, D. & Ali, M.F. Environ Sci Pollut Res (2018) 25: 15616. https://doi.org/10.1007/s11356-018-1776-x …The slides were placed on a horizontal gel electrophoresis platform (Cleaver Scientific Ltd., UK) and covered with chilled alkaline solution (300 mM NaOH and 1 mM Na2 EDTA, pH 13) in the dark at 0 °C for 10 min, electrophoresis was conducted at 0 °C in the dark for 15 min at 25 V and approximately 300 mA, and then the slides were rinsed with 400 mM Tris buffer (Wako Pure Chemical Industries, Ltd.,…
Titanium dioxide nanoparticles: Toxicity and genotoxicity in Drosophila melanogaster (SMART eye-spot test and comet assay in neuroblasts) 2018 Sara Sario, Amélia M. Silva, Isabel Gaivão, Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 831, July 2018, Pages 19-23, …ere incubated in electrophoresis buffer (0.3 M NaOH, 1 mM EDTA, pH 12.6) at 4 °C for 30 min and then subjected to electrophoresis at 0.8 V/cm and 300 mA for 30 min at 4 °C in an electrophoresis tank (cleaver scientific Ltd, Warwickshire, UK). Slides were neutralized with PBS 1× and distilled water (at 4 °C, 10 min each). Slides were stained with DAPI (1 μg/mL, 20 μL in each gel). 100 cells per gel…
DNA damage and micronuclei in parthenogenetic and bisexual Darevskia rock lizards from the areas with different levels of soil pollution 2018 Anna Simonyan, Galina Hovhannisyan, Anzhela Sargsyan, Marine Arakelyan, Rouben Aroutiounian, Ecotoxicology and Environmental Safety, Volume 154, 15 June 2018, Pages 13-18, …n lysis buffer (2.5 M NaCl, 100 mM Na2EDTA, 10 mM Tris and 1% Triton X-100, pH 10.0) for 24 h, incubated in alkaline buffer (300 mM NaOH and 1 mM Na2EDTA, pH > 13) for 20 min in electrophoresis tank (cleaver scientific, Rugby, UK) for DNA unwinding, and electrophoresed for 15 min at 300 mA and 1 V/cm in the same buffer. Slides were neutralized with 0.4 M Tris, pH 7.5 for 10 min and stained with 20…
Slight hypercalcemia is not associated with positive responses in the Comet Assay in male rat liver 2017 Anette Thiel, Annie Hamel, Katrien Schaefer, Renato Cardoso, Paul Beilstein, Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 820, August 2017, Pages 26-30, …The slides were rinsed in water and transferred to a dry electrophoresis tank. Alkaline buffer (300 mM NaOH and 1 mM EDTA disodium, pH > 13.0) was atded to the electrophoresis tank (cleaver scientific model CSL-COM40), kept to below 10 °C, in order to cover the slides of about 2–3 mm above the slides. The cells were allowed to unwind its DNA in the buffer for 20 min. A voltage of 16 V was applied …
Genotoxicity of Water Contaminants from the Basin of Lake Sevan, Armenia Evaluated by the Comet Assay in Gibel Carp (Carassius auratus gibelio) and Tradescantia Bioassays 2016 Simonyan, A., Gabrielyan, B., Minasyan, S. et al. Bull Environ Contam Toxicol (2016) 96: 309. https://doi.org/10.1007/s00128-015-1720-4 …Cl, 100 mM Na2EDTA, 10 mM Tris and 1 % Triton X-100, pH 10.0) for 60 min at 4 °C, incubated in freshly made alkaline buffer (300 mM NaOH and 1 mM Na2EDTA, pH > 13) for 20 min in electrophoresis tank (Cleaver Scientific, Rugby, UK) for DNA unwinding, and electrophoresed for 15 min at 300 mA and 1 V/cm in the same buffer. Slides were neutralized with 0.4 M Tris, pH 7.5 for 10 min and stained with 20…
Use of the rainbow trout cell lines, RTgill-W1 and RTL-W1 to evaluate the toxic potential of benzotriazoles 2016 Fanxing Zeng, James P. Sherry, Niels C. Bols, Ecotoxicology and Environmental Safety, Volume 124, February 2016, Pages 315-323, … latdering. After staining with H33258, nuclear integrity was visualized with a fluorescent microscope (Nikon Optishot). The comet assay was run with a horizontal gel electrophoresis tank (CSL-COM10, cleaver scientific). Wet electroblotting systems Mini Trans-Blot® Cell (Bio-Rad) was used for western blotting.…
The effects of lycopene on DNA damage and oxidative stress on indomethacin-induced gastric ulcer in rats 2016 Murat Boyacioglu, Cavit Kum, Selim Sekkin, Hande Sultan Yalinkilinc, Umit Karademir, Clinical Nutrition, Volume 35, Issue 2, April 2016, Pages 428-435, …C, then washed with cold PBS and introduced into a lysis solution in a separate jar for at least 1 h at 4 °C. Following lysis, slides were aligned in a horizontal gel electrophoresis tank (CSL-COM20, cleaver scientific, UK) that was connected to a recirculating cooler (FL300, Julabo, Germany) set at 4 °C and filled with freshly made alkaline electrophoresis solution (300 mM NaOH, 1 mM disodium EDT…
Use of a standardized JaCVAM in vivo rat comet assay protocol to assess the genotoxicity of three coded test compounds; ampicillin trihydrate, 1,2-dimethylhydrazine dihydrochloride, and N-nitrosodimethylamine 2015 J. P. McNamee, P. V. Bellier, Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volumes 786–788, July 2015, Pages 158-164, …er and gently rinsed with ultra-pure 18 MΩ water to remove detergent from the slides. The slides were then placed in a large (40-slide) pre-cooled horizontal electrophoresis chamber (model#CSL-COM40; cleaver scientific, UK) and submerged in cold (∼4 °C) electrophoresis buffer [containing 300 mM sodium hydroxide (Fisher Scientific Ltd.) and 1 mM disodium EDTA in purified water, pH 13.3] for 20 min …
The protective effects of vitamin C on the DNA damage, antioxidant defenses and aorta histopathology in chronic hyperhomocysteinemia induced rats 2014 Murat Boyacioglu, Selim Sekkin, Cavit Kum, Deniz Korkmaz, Ferda Akar, Experimental and Toxicologic Pathology, Volume 66, Issues 9–10, December 2014, Pages 407-413, … 4 °C, then washed with cold PBS and introduced into a lysis solution in a separate jar for at least 1 h at 4 °C. After lysis, slides were aligned in a horizontal gel electrophoresis tank (CSL-COM20, cleaver scientific, UK) connected to recirculating cooler (FL300, Julabo, Germany) set at 4 °C filled with freshly made alkaline electrophoresis solution (300 mM NaOH, 1 mM disodium EDTA, pH > 13) to …
Influence of 3-aminobenzamide, an inhibitor of poly(ADP-ribose)polymerase, in the evaluation of the genotoxicity of doxorubicin, cyclophosphamide and zidovudine in female mice 2014 L. Yadav, S. Khan, K. Shekh, G. B. Jena, Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 770, August 2014, Pages 6-15, …slides were rinsed three times in de-ionized water to remove the salt and detergent. The slides were then coded and placed in a specifically designed horizontal electrophoresis tank (Model, CSLCOM20, cleaver scientific Ltd, Warwickshire, UK) and the DNA was allowed to unwind for 20 min in an alkaline solution containing 300 mM NaOH and 1 mM EDTA (pH 13). Electrophoresis was performed at 300 mA and…
Dextran sulfate sodium-induced ulcerative colitis leads to testicular toxicity in mice: Role of inflammation, oxidative stress and DNA damage 2014 A. R. Parmar, P. P. Trivedi, G. B. Jena, Reproductive Toxicology, Volume 49, November 2014, Pages 171-184, …37.2 g) and 10 mM trizma base (1.2 g) at 4 °C for 24 h. After 24 h, the slides were washed with chilled water, then placed in a specifically designed horizontal electrophoresis tank (Model, CSLCOM20, cleaver scientific Ltd., UK), and DNA was allowed to unwind for 20 min in an alkaline solution containing 300 mM NaOH and 1 mM EDTA (pH > 13.0). Electrophoresis was conducted at 29 V, 300 mA. After ne…
The chemopreventive effect of Ginkgo biloba and Silybum marianum extracts on hepatocarcinogenesis in rats 2011 El Mesallamy, H.O., Metwally, N.S., Soliman, M.S. et al. Cancer Cell Int (2011) 11: 38. https://doi.org/10.1186/1475-2867-11-38 …riton X-100 and 10% DMSO for 24 h at 4°C. After lysis, the slides were rinsed three times in deionized water to remove salt and detergent. The slides were placed in a horizontal electrophoresis unit (Cleaver Scientific Ltd, UK) and DNA was allowed to unwind for 20 min in alkaline solution containing 300 mM NaOH and 1 mM EDTA, pH > 13. The DNA was electrophoresed for 20 min at 300 mA and 30 V (0.90…

Accessories…

nanoPAC-300P Power Supply, 300V, 400mA, 60W
NANOPAC-300P £315.00
Recirculating Chiller -20 to 100 °C
CHILLER £2,865.00