References

References for our products

Cleaver Scientific products have been used in a wide range of academic and commercial research, and published in multiple prestiguous journals. Below is a Sample of the work that has been done using our products. A more extensive list can be found here.

Reference Title:
Soy protein isolate/bioactive glass composite membranes: processing and properties
Reference Date:
2018
Citation:
Tansaz, S., Schulte, M., Kneser, U., Mohn, D., Stark, W., Roether, J.A., Cicha, I. and Boccaccini, A.R., 2018. Soy protein isolate/bioactive glass composite membranes: processing and properties. European Polymer Journal.
Product Usage:
…Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE) was carried out for MP Biomedicals SPI solution by using the OmniPAGE TETRAD Package from Cleaver Scientific Ltd. The resolving gels (10% acrylamide of about 1.5 mm thickness) were run at a constant voltage (120 V) and prepared according to the method described by Laemmli [24]. Proteins were visualized by Coomassie Brilliant blue…


Reference Title:
Biohydrogen production from palm oil mill effluent (POME) by two stage anaerobic sequencing batch reactor (ASBR) system for better utilization of carbon sources in POME
Reference Date:
2018
Citation:
Maaroff, R.M., Jahim, J.M., Azahar, A.M., Abdul, P.M., Masdar, M.S., Nordin, D. and Nasir, M.A.A., 2018. Biohydrogen production from palm oil mill effluent (POME) by two stage anaerobic sequencing batch reactor (ASBR) system for better utilization of carbon sources in POME. International Journal of Hydrogen Energy.
Product Usage:
…The PCR products were separated using VS20WAVE -DGGE (Cleaver Scientific, UK) on a vertical gel containing 8% (w/v) acrylamide with a concentration of denaturant of 30%–60% from the top to the bottom of the polyacrylamide gel. Electrophoresis was carried out in 1x TAE buffer at 140 V for 5 h at 60 °C. The gel was stained with SYBR Green Nucleic Acid Gel Stain for 40 min then visualized and sliced …


Reference Title:
Plasma Lipid Peroxidation Biomarkers For Early And Non-Invasive Alzheimer Disease Detection
Reference Date:
2018
Citation:
Peña-Bautista, C., Vigor, C., Galano, J.M., Oger, C., Durand, T., Ferrer, I., Cuevas, A., López-Cuevas, R., Baquero, M., López-Nogueroles, M. and Vento, M., 2018. PLASMA LIPID PEROXIDATION BIOMARKERS FOR EARLY AND NON-INVASIVE ALZHEIMER DISEASE DETECTION. Free Radical Biology and Medicine.
Product Usage:
…The centrifuge (multiSPIN) used was from Cleaver Scientific Ltd. (Warwickshire, United Kingdom) and the vortex mixer was from Velp Scientifica (Usmate, Italy). The speed vacuum concentrator (mi Vac) was from Genevac LTD (Ipswich, United Kingdom). The thermomixer HLC was from Ditabis (Pforzheim, Germany). The Strata X-AW (100 mg, 3 mL) solid phase extraction cartridges used for sample solid-phase e…


Reference Title:
The combination of Cassia obtusifolia L. and Foeniculum vulgare M. exhibits a laxative effect on loperamide-induced constipation of rats
Reference Date:
2018
Citation:
Jang SH, Yang DK (2018) The combination of Cassia obtusifolia L. and Foeniculum vulgare M. exhibits a laxative effect on loperamide-induced constipation of rats. PLoS ONE 13(4):
e0195624.

https://doi.org/10.1371/journal.pone.0195624

Product Usage:
…ed secondary antibodies (Cell Signaling Technology) at room temperature for 1 h and detected by using an Immobilon Western Chemiluminescence kit (Millipore Corp., Billerica, USA) and UVITEC Mini HD9 (Cleaver Scientific Ltd., Warwickshire, UK).…


Reference Title:
Human cystic echinococcosis in Turkey: a preliminary study on DNA polymorphisms of hydatid cysts removed from confirmed patients
Reference Date:
2018
Citation:
Orsten, S., Boufana, B., Ciftci, T. et al. Parasitol Res (2018) 117: 1257. https://doi.org/10.1007/s00436-018-5807-9
Product Usage:
…ed PCR products were visualised under ultraviolet light (Syngene G: Box gel documentation system, Cambridge Biosciences) following electrophoresis on 1.5% (w/v) ethidium bromide-stained agarose gels (Cleaver Scientific Limited) and purified using QIAquick PCR Purification Kit (Qiagen) according to the manufacturer’s instructions. Bi-directional commercial sequencing was achieved using the PCR prim…


Reference Title:
Development of a functionalized UV-emitting nanocomposite for the treatment of cancer using indirect photodynamic therapy
Reference Date:
2018
Citation:
Sengar, P., Juárez, P., Verdugo-Meza, A. et al. J Nanobiotechnol (2018) 16: 19. https://doi.org/10.1186/s12951-018-0344-3
Product Usage:
…es (in solution and internalized), the cells were cultured for 24 h before being exposed or not exposed to increasing doses of UV light at 365 nm (from 0.1 to 3 J/cm2), using a CL-508 UV Crosslinker (Cleaver Scientific Ltd.). UV doses were monitored using a UV radiometer (VLX-3 W, VilberLourmat). The cells were then incubated for 24 h before measuring cells viability using the MTT method or cell d…


Reference Title:
An overlooked hybrid between the two diploid Chenopodium species in Central Europe determined by microsatellite and morphological analysis
Reference Date:
2018
Citation:
Hodková, E. & Mandák, B. Plant Syst Evol (2018) 304: 295. https://doi.org/10.1007/s00606-017-1477-9
Product Usage:
…ion (Online Resource 3), was isolated using the DNeasy kit 96 Plant Kit (Qiagen) following the manufacturer’s instructions. The quality and yield of the isolated DNA were checked on a 1% agarose gel (Cleaver Scientific Ltd) and then by precisely measuring DNA concentration and purity using a NanoDrop™ 2000 spectrophotometer (Thermo Fisher Scientific). All samples were diluted to 15–20 ng/µl prior …


Reference Title:
Characterization of tumor-derived mesenchymal stem cells potentially differentiating into cancer-associated fibroblasts in lung cancer
Reference Date:
2018
Citation:
Arena, S., Salati, M., Sorgentoni, G. et al. Clin Transl Oncol (2018). https://doi.org/10.1007/s12094-018-1894-4
Product Usage:
…ively. Densitometric analyses of the immunoreactive bands (quantified as a-SMA/GAPDH bands in corresponding samples and expressed as arbitrary units, A.U.) was performed using UVITEC Imaging Systems (Cleaver Scientific Ltd, Warwickshire, United Kingdom).…


Reference Title:
Vectors for Expression of Signal Peptide-Dependent Proteins in Baculovirus/Insect Cell Systems and Their Application to Expression and Purification of the High-Affinity Immunoglobulin Gamma Fc Receptor I in Complex with Its Gamma Chain
Reference Date:
2018
Citation:
Le, L.T.M., Nyengaard, J.R., Golas, M.M. et al. Mol Biotechnol (2018) 60: 31. https://doi.org/10.1007/s12033-017-0041-8
Product Usage:
…SDS–PAGE gels were prepared following the protocol provided by the manufacturer (OmniPAGE Mini, Cleaver Scientific Ltd, Rugby, UK) with 30% acrylamide/bisacrylamide solution (Sigma-Aldrich). In the resolving gel, the acrylamide/bisacrylamide concentration was 12 or 15% according to target protein sizes, and in the stacking gel, the concentration was 4%. Samples were mixed with SDS loading buffer a…


Reference Title:
The Role of Calcium in Differentiation of Human Adipose-Derived Stem Cells to Adipocytes
Reference Date:
2018
Citation:
Goudarzi, F., Mohammadalipour, A., Khodadadi, I. et al. Mol Biotechnol (2018) 60: 279. https://doi.org/10.1007/s12033-018-0071-x
Product Usage:
…by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) in 10% gel under reducing conditions and electroblotted onto nitrocellulose membrane by a semidry transfer membrane instrument (Cleaver Scientific, Warwickshire, UK). Then, following washing the membrane by TBS buffer (20 mM Tris.Hcl, 500 mM NaCl, pH 7.4), they were blocked by 3% BSA prepared in TBST buffer (20 mM Tris.Hcl, 50…


Reference Title:
Development of Adenosine Deaminase-Specific IgY Antibodies: Diagnostic and Inhibitory Application
Reference Date:
2018
Citation:
Lupicka-Slowik, A., Psurski, M., Grzywa, R. et al. Appl Biochem Biotechnol (2018) 184: 1358. https://doi.org/10.1007/s12010-017-2626-x
Product Usage:
…ific IgYs and increased antibody avidity. For Western blot assay, calf ADA (50 ng/lane) was resolved by SDS-PAGE (4–12%, Tris-glycine) under reducing conditions and blotted (semi-dry blotting system, Cleaver Scientific, Rugby, UK) onto nitrocellulose membrane (0.45 µm, Thermo Scientific, Gdansk, Poland). The membrane was blocked with 5% skim milk in 10 mM phosphate-buffered saline with 0.05% Tween…


Reference Title:
Thidiazuron (TDZ) induced organogenesis and clonal fidelity studies in Haloxylon persicum (Bunge ex Boiss & Buhse): an endangered desert tree species
Reference Date:
2018
Citation:
Kurup, S.S., Thayale Purayil, F., Alkhaili, M.M.S. et al. Physiol Mol Biol Plants (2018). https://doi.org/10.1007/s12298-018-0532-5
Product Usage:
… 1.5% agarose gel in 1X TBE buffer and ethidium bromide as staining agent. The electrophoresis was done at 100 V for 3 h and the bands were visualized under UV light using a gel documentation system (Cleaver Scientific). PCR reactions were repeated twice to confirm the reproducibility of the results. DNA ladder (1 kb) was used as the molecular standard to confirm the appropriate size of ISSR marke…


Reference Title:
Mixed cropping regimes promote the soil fungal community under zero tillage
Reference Date:
2018
Citation:
Silvestro, L.B., Biganzoli, F., Stenglein, S.A. et al. Antonie van Leeuwenhoek (2018) 111: 1055. https://doi.org/10.1007/s10482-017-1005-5
Product Usage:
…Denaturing gradient gel electrophoresis analyses were carried out using the VS20 DGGE- mutation detection system (Cleaver Scientific Ltd, Rugby, UK). Polyacrylamide gels (8% Sigma solution) were prepared with a 10% [0.7 M urea—4% (v/v) formamide] to 50% [3.5 M urea—20% (v/v) formamide] vertical gradient using a gradient former and a peristaltic pump with a flow rate of 4 ml min-1 (Multi-Peristalti…


Reference Title:
Amidase activity is essential for medial localization of AmiC in Caulobacter crescentus
Reference Date:
2018
Citation:
Dubey, A. & Priyadarshini, R. Curr Genet (2018) 64: 661. https://doi.org/10.1007/s00294-017-0781-9
Product Usage:
…Cell lysates were analyzed by SDS–PAGE and western blot. After SDS–PAGE gels were transferred to a polyvinylidene difluoride membrane using a wet transfer apparatus (Cleaver Scientific). After overnight incubation with 5% nonfat milk in PBS at 4 °C, the membranes were washed once with PBST and incubated with antibodies against m-Cherry (Thermo Scientific) with dilution ratio (1:2000) for 1 h at ro…


Reference Title:
Discovering simple phenylboronic acid and benzoxaborole derivatives for experimental oncology – phase cycle-specific inducers of apoptosis in A2780 ovarian cancer cells
Reference Date:
2018
Citation:
Psurski, M., Lupicka-Slowik, A., Adamczyk-Wozniak, A. et al. Invest New Drugs (2018). https://doi.org/10.1007/s10637-018-0611-z
Product Usage:
…SDS-PAGE (4–12%, Tris-glycine) under reducing conditions and transferred onto a nitrocellulose membrane (pore size 0.45 µm; Thermo Fisher Scientific, Warsaw, Poland) using a semi-dry blotting system (Cleaver Scientific, Rugby, UK). The membrane was washed with Tris-buffered saline (TBS, 20 mM Tris-Base, 137 mM NaCl, pH 7.6; 5 min, room temperature) and blocked with 5% skim milk in TBS with 0.1% Tw…


Reference Title:
Development of a culture-independent method for rapid monitoring of microbial indicators in water samples
Reference Date:
2018
Citation:
Azimirad, M., Tajeddin, E., Hasani, Z. et al. Int. J. Environ. Sci. Technol. (2018). https://doi.org/10.1007/s13762-018-1680-4
Product Usage:
…Ten microlitres of amplified products was electrophoresed on 1.2% agarose gel, and then the PCR products were stained with ethidium bromide and visualized under UV light (Cleaver Scientific Ltd, Warwickshire, UK).…


Reference Title:
Metabolic and functional distinction of the Smicronyx sp. galls on Cuscuta campestris
Reference Date:
2018
Citation:
Zagorchev, L.I., Albanova, I.A., Tosheva, A.G. et al. Planta (2018). https://doi.org/10.1007/s00425-018-2926-6
Product Usage:
…ble proteins, was used for subsequent analyses. Protein concentration was measured with Pierce BCA protein assay kit according to instruction manual. A total of 15 µg protein was loaded onto wells of Cleaver Scientific Omnipage Mini electrophoretic system. Separation was on 12.5% T SDS PAGE according to the protocol of Laemmli (1970), followed by renaturation in 2.5% (v/v) Triton® X-100 for 20 min…


Reference Title:
Fibroblasts potentiate melanoma cells in vitro invasiveness induced by UV-irradiated keratinocytes
Reference Date:
2018
Citation:
Jobe, N.P., Živicová, V., Mifková, A. et al. Histochem Cell Biol (2018) 149: 503. https://doi.org/10.1007/s00418-018-1650-4
Product Usage:
…se and poured on negatively charged glass slides at density 20,000/slide. After the cell lysis and alkaline treatment (pH 13), the electrophoresis was performed in a light protected Comet Assay Tank (Cleaver Scientific, Rugby, United Kingdom) at 25 V for 30 min in the cold room at 4 °C. The nuclei were finally counterstained with 4′,6-diamidino-2-phenylindole and imaged as described above on fluor…


Reference Title:
A combinatorial approach for robust transgene delivery and targeted expression in mammary gland for generating biotherapeutics in milk, bypassing germline gene integration
Reference Date:
2018
Citation:
Ganguli, N., Ganguli, N., Chandra, S. et al. Appl Microbiol Biotechnol (2018) 102: 6221. https://doi.org/10.1007/s00253-018-9094-2
Product Usage:
… Loading volume for each sample was 15 µl along with dye. DNA was denatured at 95 °C for 10 min prior blotting on to Hybond N+ (Amersham Pharmacia Biotech, England) membrane in a slot blot apparatus (Cleaver Scientific Co., England) under vacuum. The membrane was UV cross-linked (Church and Gilbert 1984) at 12?×?104 µJ/cm2 energy in a CL-1000 Ultraviolet crosslinker (UVP, Upland, CA, USA). The cro…


Reference Title:
Assessment of neurohepatic DNA damage in male Sprague–Dawley rats exposed to organophosphates and pyrethroid insecticides
Reference Date:
2018
Citation:
Yahia, D. & Ali, M.F. Environ Sci Pollut Res (2018) 25: 15616. https://doi.org/10.1007/s11356-018-1776-x
Product Usage:
…The slides were placed on a horizontal gel electrophoresis platform (Cleaver Scientific Ltd., UK) and covered with chilled alkaline solution (300 mM NaOH and 1 mM Na2 EDTA, pH 13) in the dark at 0 °C for 10 min, electrophoresis was conducted at 0 °C in the dark for 15 min at 25 V and approximately 300 mA, and then the slides were rinsed with 400 mM Tris buffer (Wako Pure Chemical Industries, Ltd.,…


Reference Title:
The effect of chitosan–PMAA–NPK nanofertilizer on Pisum sativum plants
Reference Date:
2018
Citation:
Khalifa, N.S. & Hasaneen, M.N. 3 Biotech (2018) 8: 193. https://doi.org/10.1007/s13205-018-1221-3
Product Usage:
… electrophoresis (i.e., SDS-PAGE). Preparation and staining of the gel were carried out as described by Laemmli (1970). Electrophoresis was performed at 100 V using Omni-PAGE Mini Wide vertical unit (Cleaver Scientific, UK). Major protein bands of pea plants separated by SDS-PAGE electrophoresis were identified according to Barac et al. (2010).The experiment was repeated three times for confirming…


Reference Title:
Sourdoughs as a source of lactic acid bacteria and yeasts with technological characteristics useful for improved bakery products
Reference Date:
2018
Citation:
Martorana, A., Giuffrè, A.M., Capocasale, M. et al. Eur Food Res Technol (2018). https://doi.org/10.1007/s00217-018-3100-x
Product Usage:
…riction analyses, respectively. The gels, stained with RealSafe Nucleic Acid Staining Solution (0.5 µL/100 mL) (Real) were checked under UV transillumination and documented using the MicroDoc system (Cleaver Scientific).…


Reference Title:
Structural model of human dUTPase in complex with a novel proteinaceous inhibitor
Reference Date:
2018
Citation:
Kinga Nyíri, Haydyn D. T. Mertens, Borbála Tihanyi, Gergely N. Nagy, Bianka Kohegyi, Judit Matejka, Matthew J. Harris, Judit E. Szabó, Veronika Papp-Kádár, Veronika Németh-Pongrácz, Olivér Ozohanics, Károly Vékey, Dmitri I. Svergun, Antoni J. Borysik[…] & Beáta G. Vértessy- Show fewer authors, Scientific Reports 8, 4326
Product Usage:
… performed in Tris-Borate-EDTA (TBE) buffer for 70?min at room temperature (150?V), following 1?h pre-electrophoresis of the gel (100?V). Bands were detected with an Uvi-Tec gel documentation system (Cleaver Scientific Ltd., Ruby, UK) after staining with GelRed (Bioticum).…


Reference Title:
Quantitative assessment of individual populations within polymicrobial biofilms
Reference Date:
2018
Citation:
Susana Patrícia Lopes, Nuno Filipe Azevedo & Maria Olívia Pereira, Scientific Reports 8, 9494
Product Usage:
…mixed by inverting the tubes and centrifuged for 23?min (12000?×?g). Lastly, the supernatant was discarded and the pellet was allowed to air-dry, before resuspended it in 30?µL with RNAse free water (Cleaver Scientific Ltd, Warwickshire, UK). The concentration and purity of the total gDNA was spectrometrically assessed using a NanoDrop 1000™ (Thermo Scientific, Waltham, MA, US). The absorbance rat…


Reference Title:
A green solvent holder in electro-mediated microextraction for the extraction of phenols in water
Reference Date:
2018
Citation:
Yu Ting Chong, Marinah Mohd Ariffin, Norhayati Mohd Tahir, Saw Hong Loh, Talanta, Volume 176, 1 January 2018, Pages 558-564,
Product Usage:
…ate the samples during extraction. A custom-made tiny glass tube (50 mm × 2 mm I.D., 1 mm wall thickness) was used as the agarose gel mould. The OmniPAC Mini 300 V power supply unit was obtained from Cleaver Scientific (Warwickshire, United Kingdom). The annealed platinum wire with diameter 0.5 mm was purchased from Goodfellow Cambridge (England, United Kingdom) and applied as electrodes.…


Reference Title:
MiR-132 regulated olfactory bulb proteins linked to olfactory learning in greater short-nosed fruit bat Cynopterus sphinx
Reference Date:
2018
Citation:
Murugan Mukilan, David Mary Rajathei, Edwin Jeyaraj, Nagarajan Kayalvizhi, Koilmani Emmanuvel Rajan, Gene, Volume 671, 10 September 2018, Pages 10-20,
Product Usage:
…Equal concentrations (30?µg) of total proteins were resolved on 10% SDS-polyacrylamide gel and a semi-dry western apparatus (SD 20; Cleaver Scientific Ltd., UK) was used to transfer the separated proteins to polyvinylidene difluoride (PVDF) membrane (Millipore India Pvt. Ltd., India). Initially, membranes were blocked with 2% BSA for 3?h and then incubated with specific primary antibodies [Rabbit …


Reference Title:
Restorative effect of l-Dopa treatment against Ochratoxin A induced neurotoxicity
Reference Date:
2018
Citation:
Pratiksha V. Bhat, T. Anand, T. Mohan Manu, Farhath Khanum, Neurochemistry International, In press, corrected proof, Available online 5 April 2018,
Product Usage:
…tein levels were estimated by Lowry’s method (1951). 150?µg of protein homogenates were separated on SDS-PAGE followed by transferring onto a nitrocellulose membrane by an electro blotting apparatus (Cleaver Scientific Ltd, UK). After transferring the membranes were probed with monoclonal antibodies ß-actin (1:1000), Akt (1:1000), phospho-Akt (1:1000) and FoxO3a (1:1000) followed by incubation at …


Reference Title:
Lithium, a classic drug in psychiatry, improves nilotinib-mediated antileukemic effects
Reference Date:
2018
Citation:
Janaína Peixoto-da-Silva, Andrana K. Calgarotto, Katiucha R. Rocha, Caroline Palmeira-dos-Santos, Claudia Bincoletto, Biomedicine & Pharmacotherapy, Volume 99, March 2018, Pages 237-244,
Product Usage:
…orseradish peroxidase-linked with specific secondary antibodies (1:1000, Santa Cruz Biotechnology), visualized with ECL (GE Healthcare) and images were recorded by using a Chemidoc apparatus Uvitec, (Cleaver Scientific, UK).…


Reference Title:
PLASMA LIPID PEROXIDATION BIOMARKERS FOR EARLY AND NON-INVASIVE ALZHEIMER DISEASE DETECTION
Reference Date:
2018
Citation:
Carmen Peña-Bautista, Claire Vigor, Jean-Marie Galano, Camille Oger, Consuelo Cháfer-Pericás, Free Radical Biology and Medicine, In press, accepted manuscript, Available online 30 June 2018,
Product Usage:
…The centrifuge (multiSPIN) used was from Cleaver Scientific Ltd. (Warwickshire, United Kingdom) and the vortex mixer was from Velp Scientifica (Usmate, Italy). The speed vacuum concentrator (mi Vac) was from Genevac LTD (Ipswich, United Kingdom). The thermomixer HLC was from Ditabis (Pforzheim, Germany). The Strata X-AW (100 mg, 3 mL) solid phase extraction cartridges used for sample solid-phase e…


Reference Title:
Attenuation of cytotoxicity induced by tBHP in H9C2 cells by Bacopa monniera and Bacoside A
Reference Date:
2018
Citation:
Mohan Manu T, T. Anand, Farhath Khanum, Pathophysiology, Volume 25, Issue 2, June 2018, Pages 143-149,
Product Usage:
…cation by Lowry’s method (1951). Protein sample of 50?µg was loaded to each well to separate total cellular proteins and transferred onto a nitrocellulose membrane using an electroblotting apparatus (Cleaver Scientific Ltd, UK). Proteins transferred onto membranes were blocked with 5% milk in TBST for overnight at 4?°C. Membranes were probed with primary antibodies of GAPDH (sc-5286), Bcl2 (sc-863…


Reference Title:
Characterization and microbial analysis of first recorded observation of Conicera similis Haliday (Diptera: Phoridae) in forensic decomposition study in Romania
Reference Date:
2018
Citation:
Lavinia Iancu, Emily N. Junkins, Cristina Purcarea, Journal of Forensic and Legal Medicine, Volume 58, August 2018, Pages 50-55,
Product Usage:
…The amplicons were analyzed by electrophoresis on 1% agarose (Cleaver Scientific, England) gels. Subsequently, the PCR products were purified with PureLink® PCR Purification Kit (Invitrogen, USA) and sequenced (Macrogen, Netherlands).…


Reference Title:
H2O2 homeostasis in wild-type and ethylene-insensitive Never ripe tomato in response to salicylic acid treatment in normal photoperiod and in prolonged darkness
Reference Date:
2018
Citation:
Zoltán Takács, Péter Poór, Péter Borbély, Zalán Czékus, Irma Tari, Plant Physiology and Biochemistry, Volume 126, May 2018, Pages 74-85,
Product Usage:
…The electrophoretic separation of SOD, APX and POD isoenzymes was performed with native polyacrylamide gel electrophoresis (PAGE) using an omniPAGE electrophoresis system (Cleaver Scientific Ltd., Rugby, Warwickshire, UK). Electrophoresis was performed at 4?°C for 1–3?h?at a constant voltage of 120?V using 25?mM Tris and 192?mM glycine solution (pH 8.3) as a running buffer. For SOD and APX, 4% sta…


Reference Title:
Biosynthesis of chitosan-Oligosaccharides (COS) by non-aflatoxigenic Aspergillus sp. strain EGY1 DSM 101520: A robust biotechnological approach
Reference Date:
2018
Citation:
Amira M. Embaby, Ramy R. Melika, Ahmed Hussein, Amal H. El-Kamel, Heba S.Marey, Process Biochemistry, Volume 64, January 2018, Pages 16-30,
Product Usage:
…Fig. 4. Batch cultivation of Aspergillus sp. section Flavi strain EGY1 DSM 101520 in a laboratory scale fermenter. Production medium (4 liters) with an initial pH of 5.0 in a 7-liters Cleaver laboratory scale fermenter was inoculated with 5% (v/v) seed culture previously grown for 72 h at 30 °C. The process was conducted at 25 °C under automated controlled pH. Each data point is expressed as the a…


Reference Title:
Biosynthesis of chitosan-Oligosaccharides (COS) by non-aflatoxigenic Aspergillus sp. strain EGY1 DSM 101520: A robust biotechnological approach
Reference Date:
2018
Citation:
Amira M. Embaby, Ramy R. Melika, Ahmed Hussein, Amal H. El-Kamel, Heba S.Marey, Process Biochemistry, Volume 64, January 2018, Pages 16-30,
Product Usage:
…The process of COS biosynthesis was performed in a laboratory scale fermenter (Cleaver Scientific Ltd, UK) with 4.0 L working volume and 7.0 L full capacity. Four liters of optimized production medium were used in this experiment. Throughout the fermentation process at fixed time intervals, dissolved oxygen (DO) and pH of the culture were monitored using a dissolved oxygen probe (Autoclavable pola…


Reference Title:
Batch adsorption/desorption for purification of scFv antibodies using nanozeolite microspheres
Reference Date:
2018
Citation:
Ali Mesgari-Shadi, Mohammad-Hossein Sarrafzadeh, Baharak Divband, Jaleh Barar, Yadollah Omidi, Microporous and Mesoporous Materials, Volume 264, 1 July 2018, Pages 167-175,
Product Usage:
…The soluble scFv concentration and the adsorption/desorption portion determinations were based on the densitometry analysis using Total Lab Quant software (Cleaver Scientific, Warwickshire, United Kingdom). The purified scFvs with the defined concentrations were used to prepare the standard concentrations to obtain the calibration curve. The extracted scFvs were separated in the 12% (w/v) SDS-PAGE…


Reference Title:
Biohydrogen production from palm oil mill effluent (POME) by two stage anaerobic sequencing batch reactor (ASBR) system for better utilization of carbon sources in POME
Reference Date:
2018
Citation:
Rizal Muzhafar Maaroff, Jamaliah Md Jahim, Azratul Madihah Azahar, Peer Mohamed Abdul, Muhammad Azri Abd Nasir, International Journal of Hydrogen Energy, In press, corrected proof, Available online 30 June 2018,
Product Usage:
…The PCR products were separated using VS20WAVE -DGGE (Cleaver Scientific, UK) on a vertical gel containing 8% (w/v) acrylamide with a concentration of denaturant of 30%–60% from the top to the bottom of the polyacrylamide gel. Electrophoresis was carried out in 1x TAE buffer at 140 V for 5 h at 60 °C. The gel was stained with SYBR Green Nucleic Acid Gel Stain for 40 min then visualized and sliced …


Reference Title:
Use of aged sludge bioaugmentation in two-stage activated sludge system to enhance the biodegradation of toxic organic compounds in high strength wastewater
Reference Date:
2018
Citation:
Jarungwit Boonnorat, Somkiet Techkarnjanaruk, Ryo Honda, Sivakorn Angthong, Pradthana Prachanurak, Chemosphere, Volume 202, July 2018, Pages 208-217,
Product Usage:
…The DGGE profiles were generated from the light bands at different locations by using gel cutting tips (Cleaver Scientific, UK). The selected bands were excised from the polyacrylamide gel, re-suspended in 20?µL sterilized UltraPure™ DNase/RNase-Free Distilled Water (Invitrogen, USA), and stored overnight at 4?°C. The eluted DNA products were used as the template for re-amplification for sequencin…


Reference Title:
Titanium dioxide nanoparticles: Toxicity and genotoxicity in Drosophila melanogaster (SMART eye-spot test and comet assay in neuroblasts)
Reference Date:
2018
Citation:
Sara Sario, Amélia M. Silva, Isabel Gaivão, Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 831, July 2018, Pages 19-23,
Product Usage:
…ere incubated in electrophoresis buffer (0.3?M NaOH, 1?mM EDTA, pH 12.6) at 4?°C for 30?min and then subjected to electrophoresis at 0.8?V/cm and 300?mA for 30?min at 4?°C in an electrophoresis tank (Cleaver Scientific Ltd, Warwickshire, UK). Slides were neutralized with PBS 1× and distilled water (at 4?°C, 10?min each). Slides were stained with DAPI (1?µg/mL, 20?µL in each gel). 100 cells per gel…


Reference Title:
DNA damage and micronuclei in parthenogenetic and bisexual Darevskia rock lizards from the areas with different levels of soil pollution
Reference Date:
2018
Citation:
Anna Simonyan, Galina Hovhannisyan, Anzhela Sargsyan, Marine Arakelyan, Rouben Aroutiounian, Ecotoxicology and Environmental Safety, Volume 154, 15 June 2018, Pages 13-18,
Product Usage:
…n lysis buffer (2.5?M NaCl, 100?mM Na2EDTA, 10?mM Tris and 1% Triton X-100, pH 10.0) for 24?h, incubated in alkaline buffer (300?mM NaOH and 1?mM Na2EDTA, pH >?13) for 20?min in electrophoresis tank (Cleaver Scientific, Rugby, UK) for DNA unwinding, and electrophoresed for 15?min at 300?mA and 1?V/cm in the same buffer. Slides were neutralized with 0.4?M Tris, pH 7.5 for 10?min and stained with 20…


Reference Title:
Moringa oleifera seed lectin inhibits Ehrlich ascites carcinoma cell growth by inducing apoptosis through the regulation of Bak and NF-?B gene expression
Reference Date:
2018
Citation:
A. K. M. Asaduzzaman, Imtiaj Hasan, Aninda Chakrabortty, Sadnima Zaman, Syed Rashel Kabir, International Journal of Biological Macromolecules, Volume 107, Part B, February 2018, Pages 1936-1944,
Product Usage:
…hotometer at 260 and 280 nm. Quality of the RNAs were checked by 1.4% agarose gel electrophoresis after staining with 10 µg/ml ethidium bromide that were visualized by using gel documentation system (Cleaver Scientific Ltd, DI-HD, UK). Isolated RNA was converted to cDNA using RT Enzyme mix and RT buffer mix (Applied Biosystems, USA). The primer sequences of GAPDH, Bak, Bcl-X, NF?B and P53 were as …


Reference Title:
Secretome analysis of alkaliphilic bacterium Bacillus lehensis G1 in response to pH changes
Reference Date:
2018
Citation:
How Lie Ling, Zaidah Rahmat, Farah Diba Abu Bakar, Abdul Munir Abdul Murad, Rosli Md. Illias, Microbiological Research, Volume 215, October 2018, Pages 46-54,
Product Usage:
…1D isoelectric focusing (IEF) was carried out using an IEF 100 (Hoefer) and 2D sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was conducted using a VS20 WAVE Maxi (Cleaver Scientific Ltd). The protocols were carried according to manufacturer recommendations. Aliquots of 400?µg protein samples were used to rehydrate gel strips (Immobiline DryStrip pH 4–7, Bio-Rad) for 12?h. Af…


Reference Title:
Molecular and phylogenetic evidences of dispersion of human-infecting microsporidia to vegetable farms via irrigation with treated wastewater: One-year follow up
Reference Date:
2018
Citation:
Ehsan Javanmard, Hamed Mirjalali, Maryam Niyyati, Esfandiar Jalilzadeh, Mohammad Reza Zali, International Journal of Hygiene and Environmental Health, Volume 221, Issue 4, May 2018, Pages 642-651,
Product Usage:
…y, 5?µl of PCR products were electrophoresed on 1.5% of agarose gel in TBE (0.09?M Tris, 0.09?M boric acid, 2?mM EDTA), stained with 0.5?µg/mL ethidium bromide, and visualized with UV transluminator (Cleaver Scientific Ltd, Warwickshire, United Kingdom). All positive PCR products for Encephalitozoon spp., and genotyping amplifications were sequenced using ABI 3130 sequencer (California, USA). All …


Reference Title:
Modulation of immune response and protective efficacy of recombinant outer-membrane protein F (rOmpF) of Aeromonas hydrophila in Labeo rohita
Reference Date:
2018
Citation:
Sunita Kumari Yadav, Pujarini Dash, Pramoda Kumar Sahoo, Lalit C. Garg, Aparna Dixit, Fish & Shellfish Immunology, In press, accepted manuscript, Available online 27 June 2018,
Product Usage:
…e study [12] together with other bacteria/proteins was performed. For this, the cell lysates (1?µg each) of various Aeromonas strains were blotted onto a nitrocellulose membrane using a slot blotter (Cleaver Scientific Ltd, UK) and developed in a manner similar to western blot assay.…


Reference Title:
Effect of dry heating on physico-chemical, functional properties and digestibility of camel whey protein
Reference Date:
2018
Citation:
Shima Momen, Maryam Salami, Farhad alavi, Zahra Emam-Djomeh, Ali Akbar Moosavi-Movahedi, International Dairy Journal, In press, accepted manuscript, Available online 30 June 2018,
Product Usage:
…cetic acid:water (40:10:50, by vol) staining solution. Methanol:acetic acid:water (20:10:70, by vol) was used for gel destaining and destained gels scanned using a GDH-BASIC gel documentation system (Cleaver Scientific Ltd., Warwickshire, UK). Samples were analysed under non-reduced and reduced conditions (i.e., without and with ß-mercaptoethanol, respectively).…


Reference Title:
Assessing the impact of benzo[a]pyrene with the in vitro fish gut model: An integrated approach for eco-genotoxicological studies
Reference Date:
2018
Citation:
Laura M. Langan, Silvia Arossa, Stewart F. Owen, Awadhesh N. Jha, Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 826, February 2018, Pages 53-64,
Product Usage:
…C and then placed in pre-chilled electrophoresis buffer to unwind (1?mM EDTA, 0.3?M NaOH, pH 13). Electrophoresis was performed at 25?V, 620?mA for 25?min (1.25?V/cm) (Compac-50 HTP Comet Assay Tank, Cleaver Scientific, UK). Following electrophoresis, slides were transferred to neutralisation buffer (0.4?M Tris, adjusted to pH 7 with HCl) for min, rinsed (?×3) with distilled water, and allowed to …


Reference Title:
Experimental Mycobacterium bovis infection in three white rhinoceroses (Ceratotherium simum): Susceptibility, clinical and anatomical pathology
Reference Date:
2017
Citation:
Michel AL, Lane EP, de Klerk-Lorist L-M, Hofmeyr M, van der Heijden EMDL, Botha L, et al. (2017) Experimental Mycobacterium bovis infection in three white rhinoceroses (Ceratotherium simum): Susceptibility, clinical and anatomical pathology. PLoS ONE 12(7):
e0179943.

https://doi.org/10.1371/journal.pone.0179943

Product Usage:
…es of denaturation at 94°C (30 sec), annealing at 69°C (30 sec) and extension at 72°C (1 min) and final extension at 72°C for 10 min. PCR products were analysed using electrophoresis on a 2% agarose (Cleaver Scientific, England, UK) gel, stained with ethidium bromide (Invitrogen, Massachusetts, USA) and documented with the GelCompar Documentation System (Bio-Rad, California, USA).…


Reference Title:
A universal, rapid, and inexpensive method for genomic DNA isolation from the whole blood of mammals and birds
Reference Date:
2017
Citation:
SAHIB A. AL-SHUHAIB, M.B. J Genet (2017) 96: 171. https://doi.org/10.1007/s12041-017-0750-6
Product Usage:
…or 10 min and chilled on ice for at least 5 min. Then, the PCR amplicons were separated in a mini vertical gel electrophoresis format, gel size (W × L) cm: 10 × 10, and gel thickness: 1 mm (OmniPAGE, Cleaver Scientific, Warwickshire, UK). Denatured PCR products were loaded in 8% (37.5 acrylamide / 1 bis) polyacrylamide gels, containing 7% glycerol, and 1 × TBE buffer. The gels were run under 200 V…


Reference Title:
Development of quantum-dot-encapsulated liposome-based optical nanobiosensor for detection of telomerase activity without target amplification
Reference Date:
2017
Citation:
Zavari-Nematabad, A., Alizadeh-Ghodsi, M., Hamishehkar, H. et al. Anal Bioanal Chem (2017) 409: 1301. https://doi.org/10.1007/s00216-016-0058-z
Product Usage:
…control, the cell extracts were pre-treated by heat at 95 °C for 15 min. Therefore, telomerase was inactivated by heat shock. Finally, PCR products were separated by agarose (2%) gel electrophoresis (Cleaver Scientific Ltd, UK).…


Reference Title:
Protein Extraction and Identification by Gel Electrophoresis and Mass Spectrometry from Edible bird’s Nest Samples
Reference Date:
2017
Citation:
Zukefli, S.N., Chua, L.S. & Rahmat, Z. Food Anal. Methods (2017) 10: 387. https://doi.org/10.1007/s12161-016-0590-7
Product Usage:
…6–8) were purchased from Bio Basic Inc. (Ontario, Canada). Trypsin (sequencing grade modified trypsin) was purchased from Promega, USA. The Wide Range Protein Ladder (17 to 245 kDa) was obtained from Cleaver Scientific Ltd (Warwickshire, UK). Nanopure water (18.2 MO-cm) was produced from Barnstead NANOpure Diamond Water Purification Systems (Thermo, Waltham, MA).…


Reference Title:
Distribution and phylogenetic analysis of Blastocystis sp. subtypes isolated from IBD patients and healthy individuals in Iran
Reference Date:
2017
Citation:
Mirjalali, H., Abbasi, M.R., Naderi, N. et al. Eur J Clin Microbiol Infect Dis (2017) 36: 2335. https://doi.org/10.1007/s10096-017-3065-x
Product Usage:
…Subsequently, 10 µL of each PCR product was electrophoresed on a 1.5% agarose gel. Amplicons were stained with 0.5 µg/mL ethidium bromide and visualized using a UV Transilluminator (Cleaver Scientific Ltd., Warwickshire, United Kingdom).…


Reference Title:
The biodiversity of Lactobacillus spp. from Iranian raw milk Motal cheese and antibacterial evaluation based on bacteriocin-encoding genes
Reference Date:
2017
Citation:
Azizi, F., Habibi Najafi, M.B. & Edalatian Dovom, M.R. AMB Expr (2017) 7: 176. https://doi.org/10.1186/s13568-017-0474-2
Product Usage:
…e PCR products with 1 µl loading buffer were analyzed by electrophoresis using a 1% (w/v) agarose gel in Tris Boric acid EDTA (TBE. 1X) buffer at 100 V for 45 min. The gel was then placed in Gel doc (Cleaver Scientific Ltd) to detect the presence a band of 1500 bp. The size of the DNA fragments was estimated using a GeneRuler 100 bp Plus DNA Ladder (Fermentase, Canada).…


Reference Title:
Enhancement of gamma-aminobutyric acid (GABA) levels using an autochthonous Lactobacillus futsaii CS3 as starter culture in Thai fermented shrimp (Kung-Som)
Reference Date:
2017
Citation:
Sanchart, C., Rattanaporn, O., Haltrich, D. et al. World J Microbiol Biotechnol (2017) 33: 152. https://doi.org/10.1007/s11274-017-2317-3
Product Usage:
…tion denaturing gradient gel electrophoresis (PCR-DGGE) analysis of Kung-Som supernatant (start and end of fermentation) was carried out using the Dcode universal mutation detection system apparatus (Cleaver Scientific, Rugby, Warwickshire, UK) according to Sanchart et al. (2015). Thirty microliters of PCR product amplified from the V3 region (341 f (GC)-518r primer) were applied to 8% (w/v) polya…


Reference Title:
Internalization of (bis)phosphonate-modified cellulose nanocrystals by human osteoblast cells
Reference Date:
2017
Citation:
Gorgieva, S., Vivod, V., Maver, U. et al. Cellulose (2017) 24: 4235. https://doi.org/10.1007/s10570-017-1432-5
Product Usage:
…e control sample, being the RBITC dye solution, was prepared in parallel, using the same procedure as for the CNC-labelled dispersions. The analysis was performed on an OmniPAGE mini-SDS-PAGE system (Cleaver Scientific) with a Consort EV 215 power supply and using prepared polyacrylamide gels (Precise, Thermo scientific, Germany). In each of 12 wells, 10 µL of the sample was loaded, and a power su…


Reference Title:
Application of the rainbow trout derived intestinal cell line (RTgutGC) for ecotoxicological studies: molecular and cellular responses following exposure to copper
Reference Date:
2017
Citation:
Langan, L.M., Harper, G.M., Owen, S.F. et al. Ecotoxicology (2017) 26: 1117. https://doi.org/10.1007/s10646-017-1838-8
Product Usage:
…al melting point agarose, covered with a coverslip and dried at 4?°C. Slides were immersed in lysis solution for 1?h and then placed in electrophoresis tank to unwind (Compac-50 HTP Comet Assay Tank, Cleaver Scientific, UK). Electrophoresis was performed at 25?V, 620?mA for 25?min. Sliders were scored using an epifluoresence microscope (DMR; Leica Mi- crosystems, Milton Keynes, UK) and imaging sys…


Reference Title:
Retrotransposon distribution and copy number variation in gymnosperm genomes
Reference Date:
2017
Citation:
Voronova, A., Belevich, V., Korica, A. et al. Tree Genetics & Genomes (2017) 13: 88. https://doi.org/10.1007/s11295-017-1165-5
Product Usage:
…72 °C for 40 s, and a final elongation step at 72 °C for 10 min. Amplification products were separated by electrophoresis at 94 V for 4 h in 1× TAE buffer in a 1.5% agarose gel (LE Agarose CSL-AG500, Cleaver Scientific Ltd) with ethidium bromide. For validation, some ambiguous fragments slightly differing in length from the expected size range, or present in phylogenetically distant species, were …


Reference Title:
Programmable self-assembly of three-dimensional nanostructures from 10,000 unique components
Reference Date:
2017
Citation:
Luvena L. Ong, Nikita Hanikel, Omar K. Yaghi, Casey Grun, Maximilian T. Strauss, Patrick Bron, Josephine Lai-Kee-Him, Florian Schueder, Bei Wang, Pengfei Wang, Jocelyn Y. Kishi, Cameron Myhrvold, Allen Zhu, Ralf Jungmann, Gaetan Bellot, Yonggang Ke[…] & Peng Yin- Show fewer authors, Nature 552, 72–77
Product Usage:
…sed using 0.3%–2% agarose gel electrophoresis and stained using SYBR Safe loading dye. Gels were visualized using the Typhoon FLA 9000 gel imager and quantified using ImageJ32 or TotalLabQuant v12.2 (Cleaver Scientific).…


Reference Title:
In vitro significance of SOCS-3 and SOCS-4 and potential mechanistic links to wound healing
Reference Date:
2017
Citation:
Yi Feng, Andrew J. Sanders, Liam D. Morgan, Sioned Owen, Fiona Ruge, Keith G. Harding[…] & Wen G. Jiang- Show fewer authors, Scientific Reports 7, 6715
Product Usage:
…Tris-glycine SDS-PAGE was undertaken using an OmniPAGE VS10DSYS vertical electrophoresis system (OmniPAGE, Cleaver Scientific Ltd., Rugby, UK) to separate proteins before wet transfer to a PVDF membrane (EMD Millipore Corporation, Billerica, MA, USA).…


Reference Title:
Glycine receptor subunits expression in the developing rat retina
Reference Date:
2017
Citation:
Gustavo Sánchez-Chávez, Miguel Ángel Velázquez-Flores, Ruth Ruiz Esparza-Garrido, Rocío Salceda, Neurochemistry International, Volume 108, September 2017, Pages 177-182,
Product Usage:
…g at 12,000 × g for 10 min. The resulting pellet was washed three times with 75% ethanol and the ethanol residues were removed by evaporation. Purified RNA was suspended in 50 µl nuclease-free water (Cleaver Scientific). Concentration and purity of RNA were evaluated in the NanoDrop Spectrophotometer (Thermo Scientific).…


Reference Title:
Proteome-based identification of signal peptides for improved secretion of recombinant cyclomaltodextrin glucanotransferase in Escherichia coli
Reference Date:
2017
Citation:
How Lie Ling, Zaidah Rahmat, Abdul Munir Abdul Murad, Nor Muhammad Mahadi, Rosli Md. Illias, Process Biochemistry, Volume 61, October 2017, Pages 47-55,
Product Usage:
…IEF) was carried out using an IEF 100 (Hoefer, United States) and 2D sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE, Bio-Rad, United States) was conducted using a VS20 WAVE Maxi (Cleaver Scientific Ltd, United Kingdom). The protocols were carried out according to manufacturer recommendations. Protein spots were in-gel digested using a trypsin digestion kit (Thermo Scientific, …


Reference Title:
Assessment of CdS quantum dots effect on UV damage to DNA using a DNA/quantum dots structured electrochemical biosensor and DNA biosensing in solution
Reference Date:
2017
Citation:
Veronika Svitkova, Jana Blaskovicova, Monika Tekelova, Brigitta Margit Kallai, Jan Labuda, Sensors and Actuators B: Chemical, Volume 243, May 2017, Pages 435-444,
Product Usage:
…Gel electrophoresis was carried out using Cleaver nanoPAC-300 (Cleaver Scientific, UK) in horizontal arrangement, the subsequent visualization was performed by BioSpectrum 415 Imaging system. UV lamp OSRAM PURITEC HNS S 9 W G23 with working wavelength 253.7 nm and UV-C-radiation power of 2.5 W was used for the UV radiation.…


Reference Title:
Modulation by NADPH oxidase of the chronic cardiovascular and autonomic interaction between cyclosporine and NSAIDs in female rats
Reference Date:
2017
Citation:
Ahmed F. El-Yazbi, Karim S. Ibrahim, Hanan M. El-Gowelli, Nevine M. El-Deeb, Mahmoud M. El-Mas, European Journal of Pharmacology, Volume 806, 5 July 2017, Pages 96-104,
Product Usage:
…Protein extracts were then used for SDS-PAGE and blotting as described previously (El-Yazbi et al., 2010) with an electrophoresis setup from Cleaver Scientific Ltd. (Warwickshire, UK). Upon conclusion of the electrophoresis run, the polyacrylamide gel was cut at the 70 KD marker. The upper portion was used for transfer, while the lower portion was stained overnight at room temperature for actin in…


Reference Title:
Modulation by NADPH oxidase of the chronic cardiovascular and autonomic interaction between cyclosporine and NSAIDs in female rats
Reference Date:
2017
Citation:
Ahmed F. El-Yazbi, Karim S. Ibrahim, Hanan M. El-Gowelli, Nevine M. El-Deeb, Mahmoud M. El-Mas, European Journal of Pharmacology, Volume 806, 5 July 2017, Pages 96-104,
Product Usage:
… containing 1% sodium dodecylsulfate (SDS), 0.9% NaCl, and 80 mM Tris hydrochloride (pH 6.8). Samples were heated at 95 °C for 10 min, allowed to cool down and transferred to a rocking shaker CS-NRK (Cleaver Scientific Ltd, UK) and left overnight for protein extraction at 4 °C.…


Reference Title:
Prolonged dark period modulates the oxidative burst and enzymatic antioxidant systems in the leaves of salicylic acid-treated tomato
Reference Date:
2017
Citation:
Péter Poór, Zoltán Takács, Krisztina Bela, Zalán Czékus, Irma Tari, Journal of Plant Physiology, Volume 213, June 2017, Pages 216-226,
Product Usage:
…NADPH oxidase activity was analysed using omniPAGE electrophoresis system (Cleaver Scientific Ltd., Rugby, Warwickshire, UK) as described by Carter et al. (2007). 0.5 g of leaf samples were crushed to a fine powder in a mortar under liquid N2 and then soluble proteins were extracted by resuspending the powder in 1 mL of 50 mM sodium phosphate buffer (pH 6.8) containing 0.5% Triton X-100. The homog…


Reference Title:
Antitumor properties of a methyl-ß-d-galactopyranoside specific lectin from Kaempferia rotunda against Ehrlich ascites carcinoma cells
Reference Date:
2017
Citation:
Fazle Rabbi Shakil Ahmed, Ruhul Amin, Imtiaj Hasan, A. K. M. Asaduzzaman, Syed Rashel Kabir, International Journal of Biological Macromolecules, Volume 102, September 2017, Pages 952-959,
Product Usage:
…hotometer at 260 and 280 nm. Quality of the RNAs were checked by 1.4% agarose gel electrophoresis after staining with 10 µg/ml ethidium bromide that were visualized by using gel documentation system (Cleaver Scientific Ltd, DI-HD, UK). Isolated RNA was converted to cDNA using the RT Enzyme mix and RT buffer mix (Applied Biosystems, USA). The primer sequences of ß-actin, Bak, Bcl-2, P53 and NF?B we…


Reference Title:
Data for proteome analysis of Bacillus lehensis G1 in starch-containing medium
Reference Date:
2017
Citation:
How Lie Ling, Zaidah Rahmat, Abdul Munir Abdul Murad, Nor Muhammad Mahadi, Rosli Md. Illias, Data in Brief, Volume 14, October 2017, Pages 35-40,
Product Usage:
…ctric focusing was carried out using an IEF 100 (Hoefer, United States) and 2D sodium dodecyl sulfate-polyacrylamide gel electrophoresis (Bio-Rad, United States) was conducted using a VS20 WAVE Maxi (Cleaver Scientific Ltd, United Kingdom). The protocols were carried out according to manufacturer recommendations. Protein spots were in-gel digested using a trypsin digestion kit (Thermo Scientific, …


Reference Title:
Polyubiquitin-Photoactivatable Crosslinking Reagents for Mapping Ubiquitin Interactome Identify Rpn1 as a Proteasome Ubiquitin-Associating Subunit
Reference Date:
2017
Citation:
Michal Chojnacki, Wissam Mansour, Dharjath S. Hameed, Rajesh K. Singh, Michael H. Glickman, Cell Chemical Biology, Volume 24, Issue 4, 20 April 2017, Pages 443-457.e6,
Product Usage:
… reaction was performed in 96 well-plates, allowing for a 30 minute preincubation at 30°C. Samples were placed 10 cm from the light source and UV-irradiated for 30 min using 5X8W UV Bulbs 302/355 nm (Cleaver Scientific – UV Crosslinker). Rad23 competition reactions were conducted in PBS pH 7.4 buffer using 1 µM of Rpn1PC, 5 µM K48-Ub4+PT(73), and 0.5 µM or 1 µM of Rad23. Proteasome crosslinking wa…


Reference Title:
Identification and verification of porcine DNA in commercial gelatin and gelatin containing processed foods
Reference Date:
2017
Citation:
Hassan Ibrahem Abdullah Amqizal, Hassan Abdullah Al-Kahtani, Elsayed Ali Ismail, Khizar Hayat, Irwandi Jaswir, Food Control, Volume 78, August 2017, Pages 297-303,
Product Usage:
… conventional PCR products were analyzed by electrophoresis in 2% agarose gel in trice boric acid-EDTA buffer, stained by acridine orange, and visualized after 25 min by 70 V electrophoresis machine (Cleaver Scientific Ltd. UK). The gel document was captured by Gel Doc™ EZ Imager, (BIO-RAD, USA). The fragments of positive samples were cut by scalpel and the amplicons were purified from the gel by …


Reference Title:
Patulin Induced Oxidative Stress Mediated Apoptotic Damage in Mice, and its Modulation by Green Tea Leaves
Reference Date:
2017
Citation:
Girindrababu Venkattappa Jayashree, Krishnaswamy Krupashree, Puttasiddiah Rachitha, Farhath Khanum, Journal of Clinical and Experimental Hepatology, Volume 7, Issue 2, June 2017, Pages 127-134,
Product Usage:
…in contents were determined as mentioned earlier. Samples were (50 µg of proteins) separated by 10–15% SDS-PAGE followed by transferred to nitrocellulose membrane using an electro blotting apparatus (Cleaver Scientific Ltd, UK). The membranes were then blocked overnight at 4 °C with 5% (v/v) non-fat dry milk in Tris-buffered saline with Tween-20 (TBS-T) (10 mM Tris–HCl, 150 mM NaCl, and 0.1% Tween…


Reference Title:
Toxic compounds biodegradation and toxicity of high strength wastewater treated under elevated nitrogen concentration in the activated sludge and membrane bioreactor systems
Reference Date:
2017
Citation:
Jarungwit Boonnorat, Nimaradee Boonapatcharoen, Pradthana Prachanurak, Ryo Honda, Supaporn Phanwilai, Science of The Total Environment, Volume 592, 15 August 2017, Pages 252-261,
Product Usage:
…The DGGE profiles were created from the light bands at different locations using gel cutting tips (Cleaver Scientific, UK). The selected bands were excised from the polyacrylamide gel, re-suspended in 20 µL sterilized UltraPure™ DNase/RNase-Free Distilled Water (Invitrogen, USA), and stored overnight at 4 °C. The eluted DNA products were used as the templates for re-amplification for the sequencin…


Reference Title:
Ectoine and hydroxyectoine inhibit thermal-induced aggregation and increase thermostability of recombinant human interferon Alfa2b
Reference Date:
2017
Citation:
Faranak Salmannejad, Nastaran Nafissi-Varcheh, European Journal of Pharmaceutical Sciences, Volume 97, 15 January 2017, Pages 200-207,
Product Usage:
…erformed using a separating gel containing 12.5% (w/v) acrylamide and 0.1% SDS, and a stacking gel containing 3% (w/v) acrylamide and 0.1% SDS in the Omni PAGE mini vertical gel electrophoresis unit (Cleaver Scientific, Warwickshire, UK)·Gels were run under non-reducing condition (sample buffer without ß-mercaptoethanol) at 200 V at room temperature. Protein bands were visualized by coomassie stai…


Reference Title:
Inter- and intra-subtype variation of Blastocystis subtypes isolated from diarrheic and non-diarrheic patients in Iran
Reference Date:
2017
Citation:
Atefe Alinaghizade, Hamed Mirjalali, Mehdi Mohebali, Christen Rune Stensvold, Mostafa Rezaeian, Infection, Genetics and Evolution, Volume 50, June 2017, Pages 77-82,
Product Usage:
…h PCR product was electrophoresed on a 1.5% agarose gel in TBE buffer (0.09 M Tris, 0.09 M boric acid, 2 mM EDTA), stained with 0.5 µg/mL ethidium bromide, and visualized using a UV Transilluminator (Cleaver Scientific Ltd., Warwickshire, United Kingdom).…


Reference Title:
Assessment of CdS quantum dots effect on UV damage to DNA using a DNA/quantum dots structured electrochemical biosensor and DNA biosensing in solution
Reference Date:
2017
Citation:
Veronika Svitkova, Jana Blaskovicova, Monika Tekelova, Brigitta Margit Kallai, Jan Labuda, Sensors and Actuators B: Chemical, Volume 243, May 2017, Pages 435-444,
Product Usage:
…d stored at 4 °C. TAE (Tris-acetate-EDTA) buffer and ethidium bromide were purchased from AppliChem, Germany. Agarose, 1 kb DNA ladder used as the DNA length marker and loading dye were obtained from Cleaver Scientific Ltd, UK. Mica Grade V-1 Muscovite was purchased from SPI Supplies, USA. Poly-l-lysine hydrobromide (m.w. 30 000–70 000) was purchased from Sigma-Aldrich. Other chemicals, obtained f…


Reference Title:
Slight hypercalcemia is not associated with positive responses in the Comet Assay in male rat liver
Reference Date:
2017
Citation:
Anette Thiel, Annie Hamel, Katrien Schaefer, Renato Cardoso, Paul Beilstein, Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 820, August 2017, Pages 26-30,
Product Usage:
…The slides were rinsed in water and transferred to a dry electrophoresis tank. Alkaline buffer (300 mM NaOH and 1 mM EDTA disodium, pH > 13.0) was added to the electrophoresis tank (Cleaver Scientific model CSL-COM40), kept to below 10 °C, in order to cover the slides of about 2–3 mm above the slides. The cells were allowed to unwind its DNA in the buffer for 20 min. A voltage of 16 V was applied …


Reference Title:
Highly selective capture of minicircle DNA biopharmaceuticals by a novel zinc-histidine peptide conjugate
Reference Date:
2017
Citation:
Vítor M. Gaspar, Carla Cruz, João A. Queiroz, Chantal Pichon, Fani Sousa, Separation and Purification Technology, Volume 174, 1 March 2017, Pages 417-424,
Product Usage:
…riefly, samples were initially injected in a 1% (w/v) low-melting agarose gel stained with Safe-green™ DNA gel stain (NZYTech, Lisbon, Portugal). Electrophoresis was performed in a horizontal system (Cleaver Scientific) at 90 V for 40 min. 1 kb DNA ladder was used as control for DNA molecular weight. The gel was then imaged in a UV transilluminator equipped with a CCD camera for image acquisition …


Reference Title:
Prebiotic efficacy and mechanism of inulin combined with inulin-degrading Lactobacillus paracasei I321 in competition with Salmonella
Reference Date:
2017
Citation:
Pochanart Kanjan, Tipparat Hongpattarakere, Carbohydrate Polymers, Volume 169, 1 August 2017, Pages 236-244,
Product Usage:
…Dewulf et al. (2013). The 16S ribosomal DNA of bifidobacteria and lactobacilli was amplified using nested PCR approach before being subjected to DGGE analysis according to Uraipan et al. (2014) using Cleaver Scientific DGGE system (York, UK). The gel was stained with SYBR® Gold (Invitrogen, Grand Island, USA) before being viewed under UV transillumination system (Alpha Innotech Corporation, USA).…


Reference Title:
Nasal carriage of enterotoxigenic Staphylococcus aureus among food handlers in Kerbala city
Reference Date:
2017
Citation:
Hiba Mahdi Mahmood Alhashimi, Mohanad Mohsin Ahmed, Jassim Mohamed Mustafa, Karbala International Journal of Modern Science, Volume 3, Issue 2, June 2017, Pages 69-74,
Product Usage:
…NA, so instead of that distilled water was added. PCR reaction tubes were centrifuged briefly to mix and bring the contents to the bottom of the tubes, and placed into a thermo cycler PCR instrument (Cleaver Scientific Ltd., UK) where DNA was amplified; the thermo cycling parameters included an initial denaturation at 94 °C for 5 min, 35 cycles of 94 °C for 2 min, 57 °C for 2 min and 72 °C for 1 m…


Reference Title:
Seasonality and Locality Affect the Diversity of Anopheles gambiae and Anopheles coluzzii Midgut Microbiota from Ghana
Reference Date:
2016
Citation:
Akorli J, Gendrin M, Pels NAP, Yeboah-Manu D, Christophides GK, Wilson MD (2016) Seasonality and Locality Affect the Diversity of Anopheles gambiae and Anopheles coluzzii Midgut Microbiota from Ghana. PLoS ONE 11(6):
e0157529.

https://doi.org/10.1371/journal.pone.0157529

Product Usage:
…ns, and samples were kept at 4°C. The presence of bacteria 16S rDNA was confirmed by loading 3µL of PCR product on a 2% ethidium bromide stained agarose gel, and visualizing on a UV transilluminator (Cleaver Scientific).…


Reference Title:
Zn2+ Uptake in Streptococcus pyogenes: Characterization of adcA and lmb Null Mutants
Reference Date:
2016
Citation:
Tedde V, Rosini R, Galeotti CL (2016) Zn2+ Uptake in Streptococcus pyogenes: Characterization of adcA and lmb Null Mutants. PLoS ONE 11(3):
e0152835.

https://doi.org/10.1371/journal.pone.0152835

Product Usage:
…inc-depleted medium (THY + 35 µM TPEN) containing increasing amounts of zinc ions. The relative intensities were determined on the basis of two independent experiments using the Phoretix 1D software (Cleaver Scientific Ltd., Rugby, United Kingdom). The data were normalised on the control bands of each experiment (see Fig 6). Error bars represent the standard deviation.…


Reference Title:
In Vitro Analysis of Predicted DNA-Binding Sites for the Stl Repressor of the Staphylococcus aureus SaPIBov1 Pathogenicity Island
Reference Date:
2016
Citation:
Papp-Kádár V, Szabó JE, Nyíri K, Vertessy BG (2016) In Vitro Analysis of Predicted DNA-Binding Sites for the Stl Repressor of the Staphylococcus aureus SaPIBov1 Pathogenicity Island. PLoS ONE 11(7):
e0158793.

https://doi.org/10.1371/journal.pone.0158793

Product Usage:
…one hour, followed by loading of the samples. Electrophoresis was then performed in TBE buffer for about 60 min at room temperature at 150 V. Gels were analyzed by a Uvi-Tec gel-documentation system (Cleaver Scientific Ltd., Rugby, UK) using GelRed staining (Biotium) [3,25].…


Reference Title:
Micropropagation of a giant ornamental bromeliad Puya berteroniana through adventitious shoots and assessment of their genetic stability through ISSR primers and flow cytometry
Reference Date:
2016
Citation:
Viehmannova, I., Cepkova, P.H., Vitamvas, J. et al. Plant Cell Tiss Organ Cult (2016) 125: 293. https://doi.org/10.1007/s11240-016-0949-x
Product Usage:
…of ethidium bromide in the gel and running buffer was 0.5 µg/ml. Gels were run at 55 V for about 2.5 h at 4 V cm-1. The amplified stained products were visualized on a gel with a UV transilluminator (Cleaver Scientific, UK). The size of the amplified products was estimated using a GeneRuler 100 pb Plus DNA Ladder (Thermoscientific, Lithuania). ISSR profiles were scored visually as either having th…


Reference Title:
Cytoprotective propensity of Bacopa monniera against hydrogen peroxide induced oxidative damage in neuronal and lung epithelial cells
Reference Date:
2016
Citation:
Pandareesh, M.D., Anand, T. & Bhat, P.V. Cytotechnology (2016) 68: 157. https://doi.org/10.1007/s10616-014-9767-3
Product Usage:
…Total cellular protein was separated on SDS-PAGE and transferred onto a nitrocellulose membrane using an electro blotting apparatus (Cleaver Scientific Ltd, UK) as per earlier method (Anand et al. 2012). After transfer, the membranes were probed with a-Tubulin (sc-5286), SOD (sc-8637), a-CAT (sc-34280), a-GPx (sc-22146) and a-GR (sc-32408) (Santa Cruz Biotechnology, Santa Cruz, CA, USA) at 1: 1,00…


Reference Title:
Recombinant outer membrane protein C of Aeromonas hydrophila elicits mixed immune response and generates agglutinating antibodies
Reference Date:
2016
Citation:
Yadav, S.K., Meena, J.K., Sharma, M. et al. Immunol Res (2016) 64: 1087. https://doi.org/10.1007/s12026-016-8807-9
Product Usage:
… cells and Chinese hamster ovary cells (CHO-K1) and BSA were included as negative controls. Cell lysates (1 µg each) of various strains slot blotted onto a nitrocellulose (NC) membrane (Slot blotter, Cleaver Scientific Ltd, UK) were subjected to Western blot analysis using anti-rOmpC antisera. After blocking the non-specific sites with BSA (2 % in 1× PBS) followed by 3 washes of 10 min each with w…


Reference Title:
Mine land valorization through energy maize production enhanced by the application of plant growth-promoting rhizobacteria and arbuscular mycorrhizal fungi
Reference Date:
2016
Citation:
Moreira, H., Pereira, S.I.A., Marques, A.P.G.C. et al. Environ Sci Pollut Res (2016) 23: 6940. https://doi.org/10.1007/s11356-015-5914-4
Product Usage:
…c Acid Stain solution (Biotium Inc., USA) in 0.1 M NaCl. The DGGE images were acquired using a Safe ImagerTM Blue-Light Transilluminator (InvitrogenTM, USA) and a MicroDOCTM gel documentation system (Cleaver Scientific Ltd., UK).…


Reference Title:
Prevalence and characterization of extended-spectrum beta-lactamases producing Enterobacteriaceae in healthy children and associated risk factors
Reference Date:
2016
Citation:
Hijazi, S.M., Fawzi, M.A., Ali, F.M. et al. Ann Clin Microbiol Antimicrob (2016) 15: 3. https://doi.org/10.1186/s12941-016-0121-9
Product Usage:
…on was separated by electrophoresis in 1.5 % agarose gel for 30 min at 100 V in 0.5× TBE buffer. DNA was stained with ethidium bromide (1 µg/ml) and the bands were detected using UV transilluminator (Cleaver Scientific Ltd).…


Reference Title:
Effect of human papillomavirus 16 oncoproteins on oncostatin M upregulation in oral squamous cell carcinoma
Reference Date:
2016
Citation:
Chuerduangphui, J., Pientong, C., Chaiyarit, P. et al. Med Oncol (2016) 33: 83. https://doi.org/10.1007/s12032-016-0800-6
Product Usage:
…ord assay (Bio-Rad, Richmond, CA, USA) and separated on SDS-PAGE (12 % gel). The proteins were transferred onto nitrocellulose membranes (Bio-Rad, Hercules, CA, USA) using a semi-dry blotting system (Cleaver Scientific Ltd, UK). The membrane was incubated in blocking buffer, and primary antibody [mouse anti-p53 (Santa Cruz, CA, USA), mouse anti-ß-actin (Sigma) or rabbit anti-OSM (Sigma)] was used …


Reference Title:
Histamine development and bacterial diversity in microbially-challenged tonggol (Thunnus tonggol) under temperature abuse during canning manufacture
Reference Date:
2016
Citation:
Hongpattarakere, T., Buntin, N. & Nuylert, A. J Food Sci Technol (2016) 53: 245. https://doi.org/10.1007/s13197-015-2042-6
Product Usage:
…(5′- GC-clamp-ATTACCGCGGCTGCTGG-3′) according to Hovda et al. (2007). The 20 µl of PCR products were separated by DGGE operating under the specified condition (Uraipan et al. 2014) using DGGE system (Cleaver Scientific, York, UK). The DNA fragments were stained with SYBR® Gold (Invitrogen, Grand Island, NY, USA) and viewed by using UV transillumination (Alpha innotech corporation, San Leandro, CA,…


Reference Title:
Genotoxicity of Water Contaminants from the Basin of Lake Sevan, Armenia Evaluated by the Comet Assay in Gibel Carp (Carassius auratus gibelio) and Tradescantia Bioassays
Reference Date:
2016
Citation:
Simonyan, A., Gabrielyan, B., Minasyan, S. et al. Bull Environ Contam Toxicol (2016) 96: 309. https://doi.org/10.1007/s00128-015-1720-4
Product Usage:
…Cl, 100 mM Na2EDTA, 10 mM Tris and 1 % Triton X-100, pH 10.0) for 60 min at 4 °C, incubated in freshly made alkaline buffer (300 mM NaOH and 1 mM Na2EDTA, pH > 13) for 20 min in electrophoresis tank (Cleaver Scientific, Rugby, UK) for DNA unwinding, and electrophoresed for 15 min at 300 mA and 1 V/cm in the same buffer. Slides were neutralized with 0.4 M Tris, pH 7.5 for 10 min and stained with 20…


Reference Title:
Cognition Enhancing and Neuromodulatory Propensity of Bacopa monniera Extract Against Scopolamine Induced Cognitive Impairments in Rat Hippocampus
Reference Date:
2016
Citation:
Pandareesh, M.D., Anand, T. & Khanum, F. Neurochem Res (2016) 41: 985. https://doi.org/10.1007/s11064-015-1780-1
Product Usage:
…red by the method of Lowry et al. [57]. Tissue homogenates containing 150 µg of proteins were separated on SDS-PAGE and transferred onto a nitrocellulose membrane using an electro blotting apparatus (Cleaver Scientific Ltd, UK). After transfer, the membranes were probed with GAPDH (sc-25778) (Santa Cruz Biotechnology, CA, USA) at 1: 1000 dilutions and monoclonal anti-CREB (C8977, Sigma, St.Louis, …


Reference Title:
The Level of ALR is Regulated by the Quantity of Mitochondrial DNA
Reference Date:
2016
Citation:
Balogh, T., Lorincz, T., Stiller, I. et al. Pathol. Oncol. Res. (2016) 22: 431. https://doi.org/10.1007/s12253-015-0020-y
Product Usage:
… to the manufacturer’s protocol. 50 µg amounts of total protein samples were loaded onto 15 % polyacrylamide gel under reducing conditions and transferred to a 0.45 µm nitrocellulose membrane using a Cleaver electrophoresis and Western-blot apparatus (Cleaver Scientific, Warwickshire, UK). After Ponceau staining the membranes were blocked with Saturating-solution (5 % non-fat dry milk in TBS-Tween…


Reference Title:
Community structure of a sulfate-reducing consortium in lead-contaminated wastewater treatment process
Reference Date:
2016
Citation:
Nguyen, Y.T., Kieu, H.T., West, S. et al. World J Microbiol Biotechnol (2017) 33: 10. https://doi.org/10.1007/s11274-016-2180-7
Product Usage:
…DGGE was performed according to the manufacturer’s instructions of VS20-DGGE (Cleaver Scientific Ltd, UK). After purification, about 700 ng of PCR products was applied to 8% (w/v) polyacrylamide gels containing denaturant gradients of 40–70% (dissimilatory sulfite reductase (dsrB) gene). Whole denaturing solution contained 7 M urea and 40% formamide. Electrophoresis was run in 1 × TAE buffer (40 m…


Reference Title:
Activation and cleavage of SASH1 by caspase-3 mediates an apoptotic response
Reference Date:
2016
Citation:
Joshua T Burgess, Emma Bolderson, Mark N Adams, Anne-Marie Baird, Shu-Dong Zhang, Kathy A Gately, Kazuo Umezawa, Kenneth J O’Byrne[…] & Derek J Richard- Show fewer authors, Cell Death & Disease 7, e2469
Product Usage:
…Cells were seeded into 10?cm dishes, allowed to attach overnight and exposed to indicated doses of UVC using a UVC crosslinker (Syngene GCL8S Crosslinker UV Radiation System Cleaver Scientific; Rugby, Warwickshire, UK used with a 254?nM wavelength) or treated with 1?µM staurosporine. Live as well as necrotic and apoptotic floating cells were harvested following indicated time points.…


Reference Title:
Discovery of midgut genes for the RNA interference control of corn rootworm
Reference Date:
2016
Citation:
Xu Hu, Nina M. Richtman, Jian-Zhou Zhao, Keith E. Duncan, Xiping Niu, Lisa A. Procyk, Meghan A. Oneal, Bliss M. Kernodle, Joseph P. Steimel, Virginia C. Crane, Gary Sandahl, Julie L. Ritland, Richard J. Howard, James K. Presnail, Albert L. Lu[…] & Gusui Wu- Show fewer authors, Scientific Reports 6, 30542
Product Usage:
…nd 37?°C for siRNA, respectively. The autoradiographs were digitized by ImageQuant™ LSA4000 (Fujifilm, Tokyo, Japan). Densitometry analyses of northern blots was peformed using Phoretixs 1D software (Cleaver Scientific, Rugby, UK).…


Reference Title:
Robust generation of transgenic mice by simple hypotonic solution mediated delivery of transgene in testicular germ cells
Reference Date:
2016
Citation:
Abul Usmani, Nirmalya Ganguli, Subodh K Jain, Nilanjana Ganguli, Subeer S Majumdar, Molecular Therapy – Methods & Clinical Development, Volume 3, 2016, Article 16076,
Product Usage:
…P using High Prime DNA labeling kit (Roche Diagnostic GmbH, Mannheim, Germany). Denatured gDNA (1 µg) was blotted on Hybond N+ (Amersham Pharmacia Biotech, England) membrane with slot blot apparatus (Cleaver Scientific, Warwickshire, UK) under vacuum. The membranes were prehybridized for 4 hours, followed by hybridization with respective transgene specific probes for 10–12 hours. The hybridized bl…


Reference Title:
Kinetics of phenolic and phthalic acid esters biodegradation in membrane bioreactor (MBR) treating municipal landfill leachate
Reference Date:
2016
Citation:
Jarungwit Boonnorat, Chart Chiemchaisri, Wilai Chiemchaisri, Kazuo Yamamoto, Chemosphere, Volume 150, May 2016, Pages 639-649,
Product Usage:
…n and photographs were captured using Biovision CN 1000/26M (VilberLourmat, France). DGGE profiles were observed from obtained band light intensities at different locations by using Gel Cutting Tips (Cleaver Scientific, UK). The selected bands were excised from the polyacrylamide gel and re-suspended in 20 µL of sterilized UltraPure™DNase/RNase-Free Distilled water (Invitrogen, USA) and stored ove…


Reference Title:
The simultaneous delivery of paclitaxel and Herceptin® using solid lipid nanoparticles: In vitro evaluation
Reference Date:
2016
Citation:
Gülay Büyükköroglu, Behiye Senel, Seval Gezgin, Tan Dinh, Journal of Drug Delivery Science and Technology, Volume 35, October 2016, Pages 98-105,
Product Usage:
…ure were used as positive control. A 12% polyacrylamide gel was prepared and the samples were applied onto the gel in volumes of 40 µL.The SDS–PAGE was performed on a vertical electrophoresis system (Cleaver Scientific, UK) at constant voltage of 150 V for about 3 h. The gel was stained with Coomassie blue and de-stained in distilled water overnight. Images were obtained using a Kodak Image Statio…


Reference Title:
Biocontrol of Fusarium wilt and growth promotion of tomato plants using endophytic bacteria isolated from Nicotiana glauca organs
Reference Date:
2016
Citation:
Rania Aydi Ben Abdallah, Sonia Mokni-Tlili, Ahlem Nefzi, Hayfa Jabnoun-Khiareddine, Mejda Daami-Remadi, Biological Control, Volume 97, June 2016, Pages 80-88,
Product Usage:
…using the universal eubacterial primers 27f (5′-AGAGTTTGATC(A/C)TGGCTCAG-3′) and 1492r (5′-TACGG(C/T)TACCTTGTTACGACTT-3′) (Moretti et al., 2008). Amplifications were carried out in Thermal Cycler (CS Cleaver, Scientific Ltd., TC 32/80). The cycling conditions were as follows: one denaturing cycle at 94 °C for 4 min, followed by 40 denaturing cycles at 94 °C for 30 s, annealing at 45 °C for 30 s, a…


Reference Title:
Enzymatically-boosted ionic liquid gas separation membranes using carbonic anhydrase of biomass origin
Reference Date:
2016
Citation:
András Bednár, Nándor Nemestóthy, Péter Bakonyi, László Fülöp, Katalin Bélafi-Bakó, Chemical Engineering Journal, Volume 303, 1 November 2016, Pages 621-626,
Product Usage:
…To confirm the presence of CA, SDS-PAGE was performed on a Cleaver Scientific Ltd, Nano-PAC – 300 gel apparatus with 4% acrylamide stacking gel and 12.5% acrylamide running gel. The samples were treated with SDS and 2-mercaptoethanol before running. The proteins on the SDS-PAGE gels were stained with Coomassie Blue R-250 and visualised by a GelAnalyzer 2010a image analysis software.…


Reference Title:
Cocoa polyphenols exhibit antioxidant, anti-inflammatory, anticancerogenic, and anti-necrotic activity in carbon tetrachloride-intoxicated mice
Reference Date:
2016
Citation:
Jasminka Giacometti, Damir Muhvic, Adriano Pavletic, Luka Ðudaric, Journal of Functional Foods, Volume 23, May 2016, Pages 177-187,
Product Usage:
…PAGE sample buffer (Invitrogen, Carlsbad, CA, USA) and heated at 95?°C for 5?min. The electrophoretic separation was performed with precast 4–12% Bis–Tris gels in an OmniPAGE mini, a vertical system (Cleaver Scientific Ltd, Rugby, Warwickshire, UK) according to the manufacturer’s procedure. The gels were stained with colloidal Coomassie Blue G-250 stain and visualised by a VersaDoc Imaging System …


Reference Title:
Use of the rainbow trout cell lines, RTgill-W1 and RTL-W1 to evaluate the toxic potential of benzotriazoles
Reference Date:
2016
Citation:
Fanxing Zeng, James P. Sherry, Niels C. Bols, Ecotoxicology and Environmental Safety, Volume 124, February 2016, Pages 315-323,
Product Usage:
… laddering. After staining with H33258, nuclear integrity was visualized with a fluorescent microscope (Nikon Optishot). The comet assay was run with a horizontal gel electrophoresis tank (CSL-COM10, Cleaver Scientific). Wet electroblotting systems Mini Trans-Blot® Cell (Bio-Rad) was used for western blotting.…


Reference Title:
Quercetin glycosides and chlorogenic acid in highbush blueberry leaf decoction prevent cataractogenesis in vivo and in vitro: Investigation of the effect on calpains, antioxidant and metal chelating properties
Reference Date:
2016
Citation:
Anastasia-Varvara Ferlemi, Olga E. Makri, Penelope G. Mermigki, Fotini N. Lamari, Constantinos D. Georgakopoulos, Experimental Eye Research, Volume 145, April 2016, Pages 258-268,
Product Usage:
…Electrophoresis was performed on an instrument of Cleaver Scientific Ltd (Warwickshire, Rugby, UK) according to the modified method of Laemmli et al. (Laemmli, 1970). Lens homogenate (30 µg WSF and 250 µg WIF per lane) were mixed with sample buffer (35% v/v glycerol, 18% v/v 2-mercaptoethanol, 230 mM Tris/HCl pH = 6.8, 10% w/v sodium dodecyl sulfate, 0.3% w/v bromophenol blue), boiled for 5 min at…


Reference Title:
Extended-spectrum ß-lactamase–producing Enterobacteriaceae in cell phones of health care workers from Peruvian pediatric and neonatal intensive care units
Reference Date:
2016
Citation:
Steev Loyola, Luz R. Gutierrez, Gertrudis Horna, Kyle Petersen, Jesus Tamariz, American Journal of Infection Control, Volume 44, Issue 8, 1 August 2016, Pages 910-916,
Product Usage:
…nd blaTEM, blaSHV, and blaCTX-M genes were amplified as previously described.17, 18, 19 Polymerase chain reaction products were separated by electrophoresis on 1% agarose gel and revealed by RunSafe (Cleaver Scientific, Rugby, UK).…


Reference Title:
Identification of biogenic amines-producing lactic acid bacteria isolated from spontaneous malolactic fermentation of chilean red wines
Reference Date:
2016
Citation:
Karem Henríquez-Aedo, Daniel Durán, Apolinaria Garcia, Martha B. Hengst, Mario Aranda, LWT – Food Science and Technology, Volume 68, May 2016, Pages 183-189,
Product Usage:
…was carried out for 2 min at 72 °C. PCR products were separated by gel electrophoresis on 2% w/v agarose gel, prepared with 1X TAE (Tris acetate-EDTA, pH 8) buffer, in a MS Mini-7 horizontal chamber (Cleaver Scientific, Warwickshire, UK) applying 100V. PCR amplification patterns were visualized under a UV light using SafeView Nucleic Acid Stain (NBS Biologicals, Richmond, Canada) and 100 bp ladder…


Reference Title:
Genetic and leaf-trait variability of Vinca minor at ancient and recent localities in Central Europe
Reference Date:
2016
Citation:
Petra Hlásná Cepková, Petr Karlík, Iva Viehmannová, Vladimíra Müllerová, Michal Hejcman, Biochemical Systematics and Ecology, Volume 64, February 2016, Pages 22-30,
Product Usage:
…bH, Germany) prior to electrophoresis. The PCR products were resolved on gels in 1 × TBE buffer for 180 min at 55 V followed by 120 mA. Bands were visualised and isolated from the gel under UV light (Cleaver Scientific, UK). The size of the amplicons was estimated using the Thermo Scientific GeneRuler 100 bp Plus DNA Ladder (Thermoscientific, Lithuania).…


Reference Title:
Effect of 24-epibrassinolide on ROS content, antioxidant system, lipid peroxidation and Ni uptake in Solanum nigrum L. under Ni stress
Reference Date:
2016
Citation:
Cristiano Soares, Alexandra de Sousa, Ana Pinto, Manuel Azenha, Fernanda Fidalgo, Environmental and Experimental Botany, Volume 122, February 2016, Pages 115-125,
Product Usage:
…ls were stained with Coomassie Brilliant Blue R250 as previously described (Queirós et al., 2009). The molecular mass of protein bands were estimated by comparison with an established protein marker (Cleaver Scientific Lda). Relative RuBisCO content was performed using the method of Li et al. (2013). For each sample, gel slices corresponding to the large and small subunits of RuBisCO were incubate…


Reference Title:
Aging of low and high amylose rice at elevated temperature: Mechanism and predictive modeling
Reference Date:
2016
Citation:
Nonthacha Thanathornvarakul, Jirarat Anuntagool, Kanitha Tananuwong, Journal of Cereal Science, Volume 70, July 2016, Pages 155-163,
Product Usage:
…litres of the mixture were then loaded onto precast SDS-PAGE gel, having 10% acrylamide in separating gel and 4% acrylamide in stacking gel, and subjected to electrophoresis (OmniPAGE mini-CVS10DSYS, Cleaver Scientific, Warwickshire, UK). Perfect Protein™ markers, 10–225 kDa (Novagen®, Merck Millipore, Madison, WI, USA), were used as standard for protein molecular weight determination. Duplicate a…


Reference Title:
Identification of biogenic amines-producing lactic acid bacteria isolated from spontaneous malolactic fermentation of chilean red wines
Reference Date:
2016
Citation:
Karem Henríquez-Aedo, Daniel Durán, Apolinaria Garcia, Martha B. Hengst, Mario Aranda, LWT – Food Science and Technology, Volume 68, May 2016, Pages 183-189,
Product Usage:
…gestion for 3 h at 37 °C inside the thermocycler block. The resulting DNA fragments (patterns) were separated by gel electrophoresis on 12% v/v polyacrylamide gel in a omniPAGE mini vertical chamber (Cleaver Scientific, Warmickshire, UK) applying 80 V. RFLP patterns were visualized under UV light using 0.5 µg mL-1 of ethidium bromide with 20 bp ladder (Thermo Scientific) as a DNA molecular weight …


Reference Title:
First report of Fusarium boothii from pecan (Carya illinoinensis) and camel thorn (Vachellia erioloba) trees in South Africa
Reference Date:
2016
Citation:
M. Gryzenhout, B. Khooa, L. Landman, South African Journal of Botany, Volume 105, July 2016, Pages 158-162,
Product Usage:
…in genes were obtained following the protocols of O’Donnell et al., 2000, O’Donnell et al., 2004 using the Robust PCR kit (KAPA Biosystems). Amplification products were visualized on 1% agarose gels (Cleaver Scientific, AEC-Amersham, South Africa) containing Gelred DNA stain (Biotium, Anatech, South Africa) under UV illumination using a Geldoc XR + imaging system (Bio-Rad, South Africa).…


Reference Title:
Diversity of purple nonsulfur bacteria in shrimp ponds with varying mercury levels
Reference Date:
2016
Citation:
Kanokwan Mukkata, Duangporn Kantachote, Banjong Wittayaweerasak, Somkiet Techkarnjanaruk, Nimaradee Boonapatcharoen, Saudi Journal of Biological Sciences, Volume 23, Issue 4, July 2016, Pages 478-487,
Product Usage:
…ide gel was stained using the SYBR Gold nucleic acid stain for 20 min. The images were detected and captured on a UV transilluminator. The pufM fragments were cut from the gel using Gel Cutting Tips (Cleaver Scientific, England) and re-amplified with the pufM forward and pufM750-AT-M13 primers. The PCR products were purified using the Gel/PCR DNA fragment extraction kit (Geneaid, Taiwan) according…


Reference Title:
The effects of lycopene on DNA damage and oxidative stress on indomethacin-induced gastric ulcer in rats
Reference Date:
2016
Citation:
Murat Boyacioglu, Cavit Kum, Selim Sekkin, Hande Sultan Yalinkilinc, Umit Karademir, Clinical Nutrition, Volume 35, Issue 2, April 2016, Pages 428-435,
Product Usage:
…C, then washed with cold PBS and introduced into a lysis solution in a separate jar for at least 1 h at 4 °C. Following lysis, slides were aligned in a horizontal gel electrophoresis tank (CSL-COM20, Cleaver Scientific, UK) that was connected to a recirculating cooler (FL300, Julabo, Germany) set at 4 °C and filled with freshly made alkaline electrophoresis solution (300 mM NaOH, 1 mM disodium EDT…


Reference Title:
Evidence-Based Structural Model of the Staphylococcal Repressor Protein: Separation of Functions into Different Domains
Reference Date:
2015
Citation:
Nyíri K, Kohegyi B, Micsonai A, Kardos J, Vertessy BG (2015) Evidence-Based Structural Model of the Staphylococcal Repressor Protein: Separation of Functions into Different Domains. PLoS ONE 10(9):
e0139086.

https://doi.org/10.1371/journal.pone.0139086

Product Usage:
…ate- EDTA (TBE) buffer for 70 min at room temperature, following 1 h pre-electrophoresis of the gel. Bands were detected after staining with GelRed (Biotium), using a Uvi-Tec geldocumentation system (Cleaver Scientific Ltd., Rugby, UK).…


Reference Title:
Environmental Enrichment Reduces Anxiety by Differentially Activating Serotonergic and Neuropeptide Y (NPY)-Ergic System in Indian Field Mouse (Mus booduga): An Animal Model of Post-Traumatic Stress Disorder
Reference Date:
2015
Citation:
Ragu Varman D, Rajan KE (2015) Environmental Enrichment Reduces Anxiety by Differentially Activating Serotonergic and Neuropeptide Y (NPY)-Ergic System in Indian Field Mouse (Mus booduga): An Animal Model of Post-Traumatic Stress Disorder. PLoS ONE 10(5):
e0127945.

https://doi.org/10.1371/journal.pone.0127945

Product Usage:
…lved on SDS-polyacrylamide gel. The separated proteins were transferred electrophoretically onto polyvinylidene difluoride (PVDF) membrane (Millipore India) using a semi-dry western apparatus (SD 20; Cleaver Scientific Ltd, UK). The membranes were blocked and incubated at 4°C for 9 h with one of the following specific primary antibodies (Santa-Cruz Biotechnology, Germany and Upstate, Cell signalin…


Reference Title:
Recombinant expression of the alternate reading frame protein (ARFP) of hepatitis C virus genotype 4a (HCV-4a) and detection of ARFP and anti-ARFP antibodies in HCV-infected patients
Reference Date:
2015
Citation:
Shehat, M.G., Bahey-El-Din, M., Kassem, M.A. et al. Arch Virol (2015) 160: 1939. https://doi.org/10.1007/s00705-015-2465-4
Product Usage:
…purified recombinant ARFP and also total lysate of induced E. coli M15 (pREP4) (pQE31-ARFP) was blotted against a nitrocellulose membrane (Life Technologies, USA) using a semi-dry blotting apparatus (Cleaver Scientific, UK). After protein transfer, the membrane was left overnight in blocking buffer composed of 5 % skimmed milk in TBS buffer (0.8 % sodium chloride, 20 mM Tris-HCl, pH 7.6) at 4 °C. …


Reference Title:
Designing multilayered nanoplatforms for SERS-based detection of genetically modified organisms
Reference Date:
2015
Citation:
Uluok, S., Guven, B., Eksi, H. et al. J Nanopart Res (2015) 17: 43. https://doi.org/10.1007/s11051-014-2849-5
Product Usage:
…The electrophoresis tank was provided by Cleaver Scientific Ltd. (Rugby, United Kingdom). A Micro-Bio-Tec Brand (Giessen, Germany) electrophoresis power supply was used, and the gels were monitored using the Kodak Gel Logic 200-Imaging System (New York, NY, USA). After nanoparticle synthesis, the absorption spectra were measured using an Agilent 8453 UV–Visible spectrophotometer (Agilent Technolog…


Reference Title:
Exploring the role of the phage-specific insert of bacteriophage F11 dUTPase
Reference Date:
2015
Citation:
Nyíri, K., Papp-Kádár, V., Szabó, J.E. et al. Struct Chem (2015) 26: 1425. https://doi.org/10.1007/s11224-015-0652-2
Product Usage:
…mM boric acid and 2 mM EDTA, pH 8.3), electrophoresis was performed for 70 min at room temperature. Bands were detected after staining with GelRed (Biotium), using a Uvi-Tec gel documentation system (Cleaver Scientific Ltd., Rugby, UK).…


Reference Title:
Variability of Bacillus thuringiensis Strains by ERIC-PCR and Biofilm Formation
Reference Date:
2015
Citation:
García, K., Ibarra, J.E., Bravo, A. et al. Curr Microbiol (2015) 70: 10. https://doi.org/10.1007/s00284-014-0675-8
Product Usage:
…ined (0.5 µg/mL) 1.5 % agarose gel, ran at 70 mV in 1X TBE buffer for 4 h. Band patterns were visualized through UV illumination and a photo was taken with a digital image acquiring system (microDOC, Cleaver Scientific Ltd). Molecular size of the bands was calculated against the 100 bp DNA ladder standard (Invitrogen, USA), using the GeneTools software (Syngene, USA).…


Reference Title:
An electrochemical DNA-based biosensor to study the effects of CdTe quantum dots on UV-induced damage of DNA
Reference Date:
2015
Citation:
Hlavata, L., Striesova, I., Ignat, T. et al. Microchim Acta (2015) 182: 1715. https://doi.org/10.1007/s00604-015-1502-z
Product Usage:
…TAE (Tris-acetate-EDTA) buffer and ethidium bromide were purchased from AppliChem Germany (www.applichem.com). Agarose, 1 kb DNA ladder and Loading dye were obtained from Cleaver Scientific Ltd (www.cleaverscientific.com). 1 kb DNA ladder was used as the DNA lenght marker. Other chemicals, obtained from Mikrochem, Slovakia (www.mikrochem.com) or Lachema, Brno (www.erbalachema.com/cz), were of anal…


Reference Title:
Development of rolling circle amplification based surface-enhanced Raman spectroscopy method for 35S promoter gene detection
Reference Date:
2015
Citation:
Burcu Guven, Ismail Hakki Boyaci, Ugur Tamer, Esra Acar-Soykut, Uzeyir Dogan, Talanta, Volume 136, 1 May 2015, Pages 68-74,
Product Usage:
…The electrophoresis tank was provided by Cleaver Scientific Ltd. (Rugby, United Kingdom). Micro-Bio-Tec Brand (Giessen, Germany) was used as electrophoresis power supply and the agarose gels were monitored using the Kodak Gel Logic 200-Imaging System (New York, NY, USA). After nanoparticle synthesis, absorption spectra were measured using an Agilent 8453 UV–visible spectrophotometer (Agilent Techn…


Reference Title:
The response of Betula pubescens to inoculation with an ectomycorrhizal fungus and a plant growth promoting bacterium is substrate-dependent
Reference Date:
2015
Citation:
Nadine R. Sousa, Albina R. Franco, Miguel A. Ramos, Rui S. Oliveira, Paula M. L. Castro, Ecological Engineering, Volume 81, August 2015, Pages 439-443,
Product Usage:
…enaturing gradient ranging from 20–60% at 80 V for the ITS, or 70 V for the 16S region. The gels were photographed under a blue light transillumination table with a camera module (CSLMicrodoc System, Cleaver Scientific, UK). DGGE profiles, concerning the presence and intensity of the bands, were analyzed using Bionumerics software (version 6.5; Applied Maths, Sint-Martens-Latem, Belgium). Detected…


Reference Title:
Use of a standardized JaCVAM in vivo rat comet assay protocol to assess the genotoxicity of three coded test compounds; ampicillin trihydrate, 1,2-dimethylhydrazine dihydrochloride, and N-nitrosodimethylamine
Reference Date:
2015
Citation:
J. P. McNamee, P. V. Bellier, Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volumes 786–788, July 2015, Pages 158-164,
Product Usage:
…er and gently rinsed with ultra-pure 18 MO water to remove detergent from the slides. The slides were then placed in a large (40-slide) pre-cooled horizontal electrophoresis chamber (model#CSL-COM40; Cleaver Scientific, UK) and submerged in cold (~4 °C) electrophoresis buffer [containing 300 mM sodium hydroxide (Fisher Scientific Ltd.) and 1 mM disodium EDTA in purified water, pH 13.3] for 20 min …


Reference Title:
Differential expression patterns among heat-shock protein genes and thermal responses in the whitefly Bemisia tabaci (MEAM 1)
Reference Date:
2015
Citation:
Fernando Díaz, Rony F. Orobio, Paul Chavarriaga, Nelson Toro-Perea, Journal of Thermal Biology, Volume 52, August 2015, Pages 199-207,
Product Usage:
…ambrook et al. (2001) in the Mini-PROTEAN® Tetra System (Bio-Rad). Samples were transferred electrophoretically to a polyvinylidene difluoride (PVDF) membrane in the SD10 mini semi-dry blotting unit (Cleaver Scientific) at 200 mA/30 min. The membrane was blocked with RapidBlock™ solution (AMRESCO) and then incubated with one of a number of primary antibodies tested to detect HSP70 or HSP90 protein…


Reference Title:
Characterization of the bacterial communities of aerobic granules in a 2-fluorophenol degrading process
Reference Date:
2015
Citation:
Anouk F. Duque, Vânia S. Bessa, Paula M. L. Castro, Biotechnology Reports, Volume 5, March 2015, Pages 98-104,
Product Usage:
…cleic Acid Stain solution (Biotium Inc., USA) in 0.1 M NaCl. The DGGE images were acquired using a Safe Imager™ Blue-Light Transilluminator (Invitrogen™, USA) and a microDOC gel documentation system (Cleaver Scientific Ltd., UK). Other adaptation of this method was successfully applied to aerobic granular sludge by Winkler et al. [48].…


Reference Title:
Activity-dependent expression of miR-132 regulates immediate-early gene induction during olfactory learning in the greater short-nosed fruit bat, Cynopterus sphinx
Reference Date:
2015
Citation:
Murugan Mukilan, Durairaj Ragu Varman, Sivasubramaniam Sudhakar, Koilmani Emmanuvel Rajan, Neurobiology of Learning and Memory, Volume 120, April 2015, Pages 41-51,
Product Usage:
…DS–polyacrylamide gel. The separated proteins were transferred electrophoretically onto a polyvinylidene difluoride (PVDF) membrane (Millipore India P. Ltd) using a semi-dry western apparatus (SD 20; Cleaver Scientific Ltd, UK). The membranes were blocked and incubated at 4 °C for 8 h with one of the following specific primary antibodies (Santa-Cruz Biotechnology and Calbiochem): affinity-purified…


Reference Title:
Coagulase-negative staphylococci (CoNS) isolated from ready-to-eat food of animal origin – Phenotypic and genotypic antibiotic resistance
Reference Date:
2015
Citation:
Wioleta Chajecka-Wierzchowska, Anna Zadernowska, Beata Nalepa, Magda Sierpinska, Lucja Laniewska-Trokenheim, Food Microbiology, Volume 46, April 2015, Pages 222-226,
Product Usage:
…ncing and Oligonucleotide Synthesis (Institute of Biochemistry and Biophysics of the Polish Academy of Sciences in Warsaw, Poland) – Table 1. Electrophoretic separation: 100 V – 5 min; 70 V – 60 min (Cleaver Scientific, UK) was carried out on 1.5% agarose gel (Promega, Poland) with the addition of ethidium bromide 0.5 µg/10 ml (MP Biomedicals, Poland). Positive controls included reference strains:…


Reference Title:
Identification of Vibrio harveyi proteins involved in the specific immune response of Senegalese sole (Solea senegalensis, Kaup)
Reference Date:
2015
Citation:
A. Medina, J. M. Mancera, E. Martínez-Manzanares, M. A. Moriñigo, S. Arijo, Fish & Shellfish Immunology, Volume 47, Issue 1, November 2015, Pages 377-380,
Product Usage:
…ng a Protean Isoelectrofocusing (IEF) Cell, (BioRad). IPG strips were embedded in 0.5% agarose (w/v) on top of 10% acrylamide gel. Second dimension separations were performed on an Omnipage platform (Cleaver Scientific) at 250 V. Proteins in the gels were fixed with 50% methanol and 10% acetic acid. The gels were silver stained (Proteo Silver Plus, Sigma) and scanned at 700 ppi (GS-800, BioRad).…


Reference Title:
Using bacterial and necrophagous insect dynamics for post-mortem interval estimation during cold season: Novel case study in Romania
Reference Date:
2015
Citation:
Lavinia Iancu, David O. Carter, Emily N. Junkins, Cristina Purcarea, Forensic Science International, Volume 254, September 2015, Pages 106-117,
Product Usage:
…a final extension step of 5 min at 72 °C. A negative control for DNA amplification was carried out in the absence of insect DNA. The amplicons (710 bp) were analyzed by electrophoresis on 1% agarose (Cleaver Scientific, England) gels. After purification with QIAquick PCR Purification Kit (Qiagen), the DNA fragments were sequenced on both strands, using the cloning primers (Macrogen, Netherlands).…


Reference Title:
Presence and Analysis of Plasmids in Human and Animal Associated Arcobacter Species
Reference Date:
2014
Citation:
Douidah L, De Zutter L, Van Nieuwerburgh F, Deforce D, Ingmer H, Vandenberg O, et al. (2014) Presence and Analysis of Plasmids in Human and Animal Associated Arcobacter Species. PLoS ONE 9(1):
e85487.

https://doi.org/10.1371/journal.pone.0085487

Product Usage:
…t was size separated by electrophoresis in a 1% agarose gel with 1X TBE for 120 min at 120 V, followed by staining in 1 µg/ml ethidium bromide. An UV transilluminator and photograph system (MICROdoc, Cleaver Scientific, Ltd) with an analyst computer program (Easy software, Kodak) was used for visualization.…


Reference Title:
Water quality control in a closed re-circulating system of Pacific white shrimp (Penaeus vannamei) postlarvae co-cultured with immobilized Spirulina mat
Reference Date:
2014
Citation:
Sombatjinda, S., Wantawin, C., Techkarnjanaruk, S. et al. Aquacult Int (2014) 22: 1181. https://doi.org/10.1007/s10499-013-9738-2
Product Usage:
…luminator. The image was visualized on a UV transilluminator and was captured using Biovision CN 1000/26 M (VilberLourmat, France). Most of the bands were excised from the gel using Gel Cutting Tips (Cleaver Scientific, England) and re-amplified with the primer 338F (5′-CAC GGG GGG ACT CCT ACG GGA GGC A-3′) and the primer 518R. The PCR products were purified using a Gel/PCR DNA fragment extraction…


Reference Title:
Alkaline Hydrolysis of Chromium Tanned Leather Scrap Fibers and Anaerobic Biodegradation of the Products
Reference Date:
2014
Citation:
Ferreira, M.J., Almeida, M.F., Pinho, S.C. et al. Waste Biomass Valor (2014) 5: 551. https://doi.org/10.1007/s12649-013-9252-9
Product Usage:
…ated, by polyacrylamide gel electrophoresis in sodium dodecyl sulfate (SDS-PAGE), using a Major Scientific NM-300 N power source (Saratoga, USA), an electrophoresis system omniPage Mini Vertical from Cleaver Scientific Ld. (Warwickshire, UK) and specific consumables and standards from National Diagnostics (Yorkshire, UK). The gels were prepared and used according consumables provider instructions …


Reference Title:
Neuroprotective and Anti-Apoptotic Propensity of Bacopa monniera Extract Against Sodium Nitroprusside Induced Activation of iNOS, Heat Shock Proteins and Apoptotic Markers in PC12 Cells
Reference Date:
2014
Citation:
Pandareesh, M.D. & Anand, T. Neurochem Res (2014) 39: 800. https://doi.org/10.1007/s11064-014-1273-7
Product Usage:
…Total cellular protein, mitochondrial and cytosolic protein fractions were separated on SDS-PAGE and transferred onto a nitrocellulose membrane using an electro blotting apparatus (Cleaver Scientific Ltd., UK) as per our earlier studies [11]. After transfer, the membranes were probed with a-Tubulin B-7 (sc-5286), HSP-70 C92F3A (sc-66048), Bcl-2 (sc-7382), Bax (sc-70408), cytochrome c (sc-13156) (S…


Reference Title:
Anti-apoptotic mechanism of Bacoside rich extract against reactive nitrogen species induced activation of iNOS/Bax/caspase 3 mediated apoptosis in L132 cell line
Reference Date:
2014
Citation:
Anand, T., Pandareesh, M.D., Bhat, P.V. et al. Cytotechnology (2014) 66: 823. https://doi.org/10.1007/s10616-013-9634-7
Product Usage:
…Total cellular protein was separated on SDS-PAGE and transferred onto a nitrocellulose membrane using an electro blotting apparatus (Cleaver Scientific Ltd, Rugby, UK) as per earlier method (Anand et al. 2012). After transfer, the membranes were probed with a-tubulin B-7 (sc-5286), anti HSP-70 C92F3A (sc-66048), Bcl2 (sc-7382), Bax (sc-70408), cytochrome c (sc-13156) (Santa Cruz Biotechnology, San…


Reference Title:
Phytomanagement of Cd-contaminated soils using maize (Zea mays L.) assisted by plant growth-promoting rhizobacteria
Reference Date:
2014
Citation:
Moreira, H., Marques, A.P.G.C., Franco, A.R. et al. Environ Sci Pollut Res (2014) 21: 9742. https://doi.org/10.1007/s11356-014-2848-1
Product Usage:
…c Acid Stain solution (Biotium Inc., USA) in 0.1 M NaCl. The DGGE images were acquired using a Safe ImagerTM Blue-Light Transilluminator (InvitrogenTM, USA) and a MicroDOCTM gel documentation system (Cleaver Scientific Ltd, UK).…


Reference Title:
Characterization of immune response elicited by the recombinant outer membrane protein OmpF of Aeromonas hydrophila, a potential vaccine candidate in murine model
Reference Date:
2014
Citation:
Yadav, S.K., Sahoo, P.K. & Dixit, A. Mol Biol Rep (2014) 41: 1837. https://doi.org/10.1007/s11033-014-3033-9
Product Usage:
…nas isolates together with other bacteria/proteins was performed. For this, the cell lysates (1 µg each) of various Aeromonas strains were blotted onto a nitrocellulose membrane using a slot blotter (Cleaver Scientific Ltd, UK). BSA (2 %) made in 1 × PBST was used for blocking the non-specific sites for 1 h at 37 °C. The membrane was washed thrice with washing buffer (0.15 M PBS, pH 7.3, and 0.2 %…


Reference Title:
Bacterial community dynamics in a rotating biological contactor treating 2-fluorophenol-containing wastewater
Reference Date:
2014
Citation:
Duque, A.F., Bessa, V.S. & Castro, P.M.L. J Ind Microbiol Biotechnol (2014) 41: 97. https://doi.org/10.1007/s10295-013-1381-4
Product Usage:
…lGreen nucleic acid stain solution (Biotium Inc., USA) in 0.1 M NaCl. Images were acquired using a Safe Imager™ blue-light transilluminator (Invitrogen™, USA) and a microDOC gel documentation system (Cleaver Scientific Ltd, UK).…


Reference Title:
Novel method for the high-throughput processing of slides for the comet assay
Reference Date:
2014
Citation:
Mahsa Karbaschi & Marcus S. Cooke, Scientific Reports 4, 7200
Product Usage:
…roof-of-principle, but required the HT rack to be shortened to fit into the tank. The tank design was then improved upon so as to accommodate two, full size HT racks (named HT Tank 2; manufactured by Cleaver Scientific Ltd, Rugby, UK), and used for all subsequent experiments involving further testing of the HT rack (Figure 2C, right).…


Reference Title:
Characterization of Staphylococcus epidermidis phage vB_SepS_SEP9 – a unique member of the Siphoviridae family
Reference Date:
2014
Citation:
Luís D. R. Melo, Sanna Sillankorva, Hans-Wolfgang Ackermann, Andrew M. Kropinski, Nuno Cerca, Research in Microbiology, Volume 165, Issue 8, October 2014, Pages 679-685,
Product Usage:
… previous step, an equal volume of chloroform was added. The supernatant was precipitated on ice with isopropanol 3 M sodium acetate (pH 4.6), air-dried and it was resuspended in nuclease-free water (Cleaver Scientific, Rugby, UK). Genome sequencing was performed on a 454 sequencing platform (Plate-forme d’Analyses Génomiques at Laval University, Québec, Canada) to 50-fold coverage. Sequence data …


Reference Title:
The protective effects of vitamin C on the DNA damage, antioxidant defenses and aorta histopathology in chronic hyperhomocysteinemia induced rats
Reference Date:
2014
Citation:
Murat Boyacioglu, Selim Sekkin, Cavit Kum, Deniz Korkmaz, Ferda Akar, Experimental and Toxicologic Pathology, Volume 66, Issues 9–10, December 2014, Pages 407-413,
Product Usage:
… 4 °C, then washed with cold PBS and introduced into a lysis solution in a separate jar for at least 1 h at 4 °C. After lysis, slides were aligned in a horizontal gel electrophoresis tank (CSL-COM20, Cleaver Scientific, UK) connected to recirculating cooler (FL300, Julabo, Germany) set at 4 °C filled with freshly made alkaline electrophoresis solution (300 mM NaOH, 1 mM disodium EDTA, pH > 13) to …


Reference Title:
Influence of 3-aminobenzamide, an inhibitor of poly(ADP-ribose)polymerase, in the evaluation of the genotoxicity of doxorubicin, cyclophosphamide and zidovudine in female mice
Reference Date:
2014
Citation:
L. Yadav, S. Khan, K. Shekh, G. B. Jena, Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 770, August 2014, Pages 6-15,
Product Usage:
…slides were rinsed three times in de-ionized water to remove the salt and detergent. The slides were then coded and placed in a specifically designed horizontal electrophoresis tank (Model, CSLCOM20, Cleaver Scientific Ltd, Warwickshire, UK) and the DNA was allowed to unwind for 20 min in an alkaline solution containing 300 mM NaOH and 1 mM EDTA (pH 13). Electrophoresis was performed at 300 mA and…


Reference Title:
Environmental enrichment modulates glucocorticoid receptor expression and reduces anxiety in Indian field male mouse Mus booduga through up-regulation of microRNA-124a
Reference Date:
2014
Citation:
Ragu Varman Durairaj, Emmanuvel Rajan Koilmani, General and Comparative Endocrinology, Volume 199, 1 April 2014, Pages 26-32,
Product Usage:
…cyl sulfate–polyacrylamide gel. The separated proteins were transferred electrophoretically onto polyvinylidene difluoride (PVDF) membrane (Millipore India) using a semi-dry western apparatus (SD 20; Cleaver Scientific Ltd, UK). The membranes were then preblocked with Tris-buffered saline [10 mM Tris-base pH-7.5, 150 mM Nacl (TBS) containing non fat dry milk (5%) and tween 20 (0.1%)] for 3 h at RT…


Reference Title:
Attenuation of smoke induced neuronal and physiological changes by bacoside rich extract in Wistar rats via down regulation of HO-1 and iNOS
Reference Date:
2014
Citation:
M. D. Pandareesh, T. Anand, NeuroToxicology, Volume 40, January 2014, Pages 33-42,
Product Usage:
…ed by the method of Lowry et al. (1951). Tissue homogenates containing 50 µg of proteins were separated on SDS-PAGE and transferred onto a nitrocellulose membrane using an electro blotting apparatus (Cleaver Scientific Ltd, UK). After transfer, the membranes were probed with primary antibodies against a-Tubulin B-7 (sc-5286) and HSP-70 C92F3A (sc-66048) (Santa Cruz Biotechnology, CA, USA) at 1: 10…


Reference Title:
Dextran sulfate sodium-induced ulcerative colitis leads to testicular toxicity in mice: Role of inflammation, oxidative stress and DNA damage
Reference Date:
2014
Citation:
A. R. Parmar, P. P. Trivedi, G. B. Jena, Reproductive Toxicology, Volume 49, November 2014, Pages 171-184,
Product Usage:
…37.2 g) and 10 mM trizma base (1.2 g) at 4 °C for 24 h. After 24 h, the slides were washed with chilled water, then placed in a specifically designed horizontal electrophoresis tank (Model, CSLCOM20, Cleaver Scientific Ltd., UK), and DNA was allowed to unwind for 20 min in an alkaline solution containing 300 mM NaOH and 1 mM EDTA (pH > 13.0). Electrophoresis was conducted at 29 V, 300 mA. After ne…


Reference Title:
Antagonistic mechanisms of synbiosis between Lactobacillus plantarum CIF17AN2 and green banana starch in the proximal colon model challenged with Salmonella Typhimurium
Reference Date:
2014
Citation:
Supansa Uraipan, Patrizia Brigidi, Tipparat Hongpattarakere, Anaerobe, Volume 28, August 2014, Pages 44-53,
Product Usage:
…ectively. The conditions for separation of PCR amplicons were 60 °C for 16 h with electrophoresis at constant voltage of 70 V and 85 V for LAB and bifidobacteria, respectively [32] using DGGE system (Cleaver Scientific, York, UK). The DNA fragments were stained with SYBR® Gold (Invitrogen, Grand Island, NY, USA) and then viewed under UV transillumination (Alpha innotech corporation, San Leandro, C…


Reference Title:
Effect of progesterone on Candida albicans vaginal pathogenicity
Reference Date:
2014
Citation:
Carlos Tiago Alves, Sónia Silva, Leonel Pereira, David W. Williams, Mariana Henriques, International Journal of Medical Microbiology, Volume 304, Issue 8, November 2014, Pages 1011-1017,
Product Usage:
… gene. Each reaction mixture consisted of 10 µL (working concentration) of SsoFast EvaGreen Supermix (Bio-Rad, Berkeley, USA), 0.2 µL of forward and reverse primers (50 µM) (Table 1), 5.6 µL of dH2O (Cleaver Scientific Ltd, UK), 4 µL of two-fold diluted cDNA samples together with the respective target gene primers. Negative controls (dH2O), as well as, non- reverse transcriptase controls (NRT) wer…


Reference Title:
Performance of aerobic granular sludge in a sequencing batch bioreactor exposed to ofloxacin, norfloxacin and ciprofloxacin
Reference Date:
2014
Citation:
Catarina L. Amorim, Alexandra S. Maia, Raquel B. R. Mesquita, António O. S. S. Rangel, Paula M. L. Castro, Water Research, Volume 50, 1 March 2014, Pages 101-113,
Product Usage:
…cleic Acid Stain solution (Biotium Inc., USA) in 0.1 M NaCl. The DGGE images were acquired using a Safe Imager™ Blue-Light Transilluminator (Invitrogen™, USA) and a microDOC gel documentation system (Cleaver Scientific Ltd, UK).…


Reference Title:
Sulphate fertilization ameliorates long-term aluminum toxicity symptoms in perennial ryegrass (Lolium perenne)
Reference Date:
2014
Citation:
Cristian Wulff-Zottele, Holger Hesse, Joachim Fisahn, Mariusz Bromke, Maria de la Luz Mora, Plant Physiology and Biochemistry, Volume 83, October 2014, Pages 88-99,
Product Usage:
…isolation the 12% SDS gels were stained with colloidal Comassie (Invitrogen, USA) over night and distained for 3 h in water. Image acquisition was performed using a microDOC Gel documentation system (Cleaver Scientific Ltd, Warwickshire, UK). Modifications in the protein accumulation were quantified through the ImageJ program to establish changes in the expression levels of proteins (ImageJ 1.23J;…


Reference Title:
Purification of papain from unclarified papaya juice using reversed phase expanded bed adsorption chromatography (RP-EBAC)
Reference Date:
2014
Citation:
Yuhai He, Syed Mohd Saufi bin Tuan Chik, Fui Chin Chong, Journal of Industrial and Engineering Chemistry, Volume 20, Issue 6, 25 November 2014, Pages 4293-4297,
Product Usage:
…al protein in each sample fraction was quantified using the Bradford Assay. The collected fractions of papain were subjected to electrophoresis at 200 V on a CSL OmniPAGE Mini Electrophoresis System (Cleaver Scientific Ltd., Rugby, UK) with acrylamide of 15% (w/v) and 5% (w/v) for resolving gel and stacking gel respectively.…


Reference Title:
Vitamin-D receptor (VDR) gene polymorphisms (Taq-I & Apa-I) in Syrian healthy population
Reference Date:
2014
Citation:
Shaden HADDAD, Meta Gene, Volume 2, December 2014, Pages 646-650,
Product Usage:
… agarose gel containing 0.5 µg/ml ethidium bromide using a gel electrophoresis system (Pequlab, Germany) at 100 V for 20–30 min. The gel was visualized under UV light using a transilluminator system (Cleaver Scientific Ltd., UK).…


Reference Title:
Phosphate-solubilizing rhizobacteria enhance Zea mays growth in agricultural P-deficient soils
Reference Date:
2014
Citation:
Sofia I. A. Pereira, Paula M. L. Castro, Ecological Engineering, Volume 73, December 2014, Pages 526-535,
Product Usage:
…el-Green Nucleic Acid Stain solution (Biotium, Inc.) for 10 min. The DGGE images were acquired using a Safe Imager™ Blue-Light Transilluminator (Invitrogen™) and a micro DOC gel documentation system (Cleaver Scientific Ltd.). Every gel contained a standard with seven bands for internal and external normalization and a lane with the profiles of the three pure bacterial cultures for comparison with …


Reference Title:
Effect of progesterone on Candida albicans vaginal pathogenicity
Reference Date:
2014
Citation:
Carlos Tiago Alves, Sónia Silva, Leonel Pereira, David W. Williams, Mariana Henriques, International Journal of Medical Microbiology, Volume 304, Issue 8, November 2014, Pages 1011-1017,
Product Usage:
…), 0.2 µL of each primer (50 µM) designed previously (Table 1)) and 4 µL of DNA, in a final reaction volume of 20 µL. Negative controls were performed using a reaction mixture with nuclease free H2O (Cleaver Scientific Ltd, UK) substituting for the template DNA. Template DNA for each positive control was obtained from FFPE tissues after the step of DNA extraction described above. PCR cycling condi…


Reference Title:
Shedding Light on the Microbial Community of the Macropod Foregut Using 454-Amplicon Pyrosequencing
Reference Date:
2013
Citation:
Gulino L-M, Ouwerkerk D, Kang AYH, Maguire AJ, Kienzle M, Klieve AV (2013) Shedding Light on the Microbial Community of the Macropod Foregut Using 454-Amplicon Pyrosequencing. PLoS ONE 8(4):
e61463.

https://doi.org/10.1371/journal.pone.0061463

Product Usage:
… Products were visualised on a 2% agarose gel post stained with 3 Xs gel red (Jomar Scientific, Australia). Amplicons of correct size (approximately 520 bp) were excised using GelX 6.5 excision tips (Cleaver Scientific, UK) and purified using the QIAquick gel extraction kit (QIAGEN, Australia) as per manufacturer’s instructions, with a modification to the final elution step, eluting the product in…


Reference Title:
l-Dopa decarboxylase (DDC) constitutes an emerging biomarker in predicting patients’ survival with stomach adenocarcinomas
Reference Date:
2013
Citation:
Florou, D., Papadopoulos, I.N., Fragoulis, E.G. et al. J Cancer Res Clin Oncol (2013) 139: 297. https://doi.org/10.1007/s00432-012-1326-8
Product Usage:
…Fifty micrograms of total protein was separated on a 10 % SDS-polyacrylamide gel electrophoresis (SDS-PAGE) apparatus (Cleaver Scientific, UK) (Laemmli 1970) at 150 V for 1.5 h at room temperature. Transferring of proteins onto nitrocellulose sheets (Scheicher and Schuell Bioscience, Germany) (Towbin et al. 1979) took place inside a semidry apparatus (BioRad, UK). Membrane blockage in 10 % BSA occ…


Reference Title:
Inoculating Helianthus annuus (sunflower) grown in zinc and cadmium contaminated soils with plant growth promoting bacteria – Effects on phytoremediation strategies
Reference Date:
2013
Citation:
Ana P. G. C. Marques, Helena Moreira, Albina R. Franco, António O. S. S. Rangel, Paula M. L. Castro, Chemosphere, Volume 92, Issue 1, June 2013, Pages 74-83,
Product Usage:
…cleic Acid Stain solution (Biotium Inc., USA) in 0.1 M NaCl. The DGGE images were acquired using a Safe Imager™ Blue-Light Transilluminator (Invitrogen™, USA) and a microDOC gel documentation system (Cleaver Scientific Ltd., UK).…


Reference Title:
Molecular characterisation and infection dynamics of Dentitruncus truttae from trout (Salmo trutta and Oncorhynchus mykiss) in Krka River, Croatia
Reference Date:
2013
Citation:
Vardic Smrzlic Irena, Valic Damir, Kapetanovic Damir, Dragun Zrinka, Teskeredžic Emin, Veterinary Parasitology, Volume 197, Issues 3–4, 8 November 2013, Pages 604-613,
Product Usage:
… on a 9% acrylamide–bisacrylamide (29:1) non-denaturing gel (16 cm × 18 cm × 0.1 cm) without glycerol. Electrophoresis was carried out at 200 V for 8 h at 8 °C in a vertical apparatus (OmniPAGE Maxi, Cleaver Scientific). Bands were visualised by ethidium bromide staining under UV (UV Transilluminator, BioRad) and photographed (GelCam, Polaroid).…


Reference Title:
Genetic diversity of yacon (Smallanthus sonchifolius (Poepp. & Endl.) H. Robinson) and its wild relatives as revealed by ISSR markers
Reference Date:
2013
Citation:
Eva Svobodová, Zdislava Dvoráková, Petra Hlásná Cepková, Iva Viehmannová, Gregorio Meza Zela, Biochemical Systematics and Ecology, Volume 50, October 2013, Pages 383-389,
Product Usage:
…ly separated using 2% agarose gel and visualised by staining with SYBR® Safe DNA Gel (Life technologies, USA). The electrophoretic patterns of PCR products were recorded digitally using CLS-Microdoc (Cleaver Scientific, UK). As a negative control used in every PCR reaction served a sample, in which the DNA template was replaced by distilled water.…


Reference Title:
DNA damage in hair root cells as a biomarker for gamma ray exposure
Reference Date:
2013
Citation:
Semra Tepe Çam, Nesrin Seyhan, Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 756, Issues 1–2, 30 August 2013, Pages 201-205,
Product Usage:
…ere immersed for 1 h in a lysing solution (2.5 M NaCl, 100 mM EDTA, 10 mM Tris, 1% Triton X-100, 10% DMSO) at 4 °C. The slides were then placed in an electrophoresis unit (MSCHOICE, MP-250 N omniPAC, Cleaver Scientific Ltd., Warwickshire, UK) containing a buffer (300 mM NaOH, 1 mM EDTA, pH 13) for 40 min at room temperature to allow for DNA unwinding. Electrophoresis was then carried out for 30 mi…


Reference Title:
Bioaugmentation for treating transient 4-fluorocinnamic acid shock loads in a rotating biological contactor
Reference Date:
2013
Citation:
Catarina L. Amorim, Anouk F. Duque, Carlos M. M. Afonso, Paula M. L. Castro, Bioresource Technology, Volume 144, September 2013, Pages 554-562,
Product Usage:
…eic Acid Stain solution (Biotium Inc., USA) in 0.1 M NaCl. The DGGE images were acquired using a Safe ImagerTM Blue-Light Transilluminator (InvitrogenTM, USA) and a microDOC gel documentation system (Cleaver Scientific Ltd, UK).…


Reference Title:
Carbendazim dissipation in the biomixture of on-farm biopurification systems and its effect on microbial communities
Reference Date:
2013
Citation:
G. R. Tortella, R. A. Mella-Herrera, D. Z. Sousa, O. Rubilar, M. C. Diez, Chemosphere, Volume 93, Issue 6, October 2013, Pages 1084-1093,
Product Usage:
…The resulting PCR products from the bacteria, fungi and Actinomycetes were then subjected to DGGE analysis using an omniPAGE-DGGE (Cleaver Scientific, Warwickshire, UK). Linear denaturing gradients of 8% (w/v) polyacrylamide gel between 40% and 65% (for bacteria), 30% and 70% (for fungi) and 30–60% (for Actinomycetes) (urea and formamide) were prepared using the GM-100 Linear Gradient Maker (CBS S…


Reference Title:
Effects of heavy metals on antioxidants and expression of HSP70 in different tissues of Milk fish (Chanos chanos) of Kaattuppalli Island, Chennai, India
Reference Date:
2013
Citation:
Sivakumar Rajeshkumar, Jayaprakash Mini, Natesan Munuswamy, Ecotoxicology and Environmental Safety, Volume 98, 1 December 2013, Pages 8-18,
Product Usage:
… (Pall Corporation, East Hills, New York) at 15 V for 90 min using alkaline buffer (48 mM Tris, 39 mM glycine, 0.037 percent (w/v) SDS, 20 percent methanol v/v, pH 8.3) and a semi dry blotting units (Cleaver Scientific Ltd., Rugby, UK). The PVDF blots were blocked with TBST (10 mM Tris, 150 mM NaCl, 1.54 mM sodium azide, 0.05 percent (v/v) Tween-20, pH 7.5) containing 5 percent (w/v) skimmed milk …


Reference Title:
Performance of an aerobic granular sequencing batch reactor fed with wastewaters contaminated with Zn2+
Reference Date:
2013
Citation:
Ana P. G. C. Marques, Anouk F. Duque, Vânia S. Bessa, Raquel B. R. Mesquita, Paula M. L. Castro, Journal of Environmental Management, Volume 128, 15 October 2013, Pages 877-882,
Product Usage:
…cleic Acid Stain solution (Biotium Inc., USA) in 0.1 M NaCl. The DGGE images were acquired using a Safe Imager™ Blue-Light Transilluminator (Invitrogen™, USA) and a microDOC gel documentation system (Cleaver Scientific Ltd, UK).…


Reference Title:
Atrazine dissipation and its impact on the microbial communities and community level physiological profiles in a microcosm simulating the biomixture of on-farm biopurification system
Reference Date:
2013
Citation:
G. R. Tortella, R. A. Mella-Herrera, D. Z. Sousa, O. Rubilar, M. C. Diez, Journal of Hazardous Materials, Volume 260, 15 September 2013, Pages 459-467,
Product Usage:
…PCR products from bacteria, fungi and actinomycetes were subject to DGGE analysis using an omniPAGE-DGGE (Cleaver Scientific, Warwickshire, UK). An 8% (w/v) polyacrylamide gel containing a linear denaturing gradient between 40–65% (for bacteria), 30–70% (for fungi) and 30–60% (for actinomycetes) of urea and formamide was prepared using a GM-100 Linear Gradient Maker (CBS Scientific, UK). Twenty µL…


Reference Title:
Non structural protein of avian influenza A (H11N1) virus is a weaker suppressor of immune responses but capable of inducing apoptosis in host cells
Reference Date:
2012
Citation:
Mukherjee, S., Majumdar, S., Vipat, V.C. et al. Virol J (2012) 9: 149. https://doi.org/10.1186/1743-422X-9-149
Product Usage:
…0 µg) from cell extracts were separated by 12.5% SDS-polyacrylamide gel electrophoresis (12.5% SDS-PAGE) and transferred to Hybond-C (Amersham Biosciences) membrane with an electrotransfer apparatus (Cleaver Scientific Ltd) at 10 Volts (100 mA) for 1 h. The membrane was probed with specific monoclonal antibodies against Influenza A-NS1 protein (Santa Cruz Biotechnology, Inc. Santa Cruz, CA). Prima…


Reference Title:
Comparison of the Anti-inflammatory and Anti-nociceptive Effects of Cortistatin-14 and Somatostatin-14 in Distinct In Vitro and In Vivo Model Systems
Reference Date:
2012
Citation:
Markovics, A., Szoke, É., Sándor, K. et al. J Mol Neurosci (2012) 46: 40. https://doi.org/10.1007/s12031-011-9577-4
Product Usage:
…specific binding was subtracted from total binding. The reaction was terminated by filtrating the samples through Whatman GF/B glass fiber filters (Whatman, UK). Using 48-well Slot Blot Manifold from Cleaver Scientific filters were washed three times with ice-cold 50 mM Tris–HCl buffer (pH 7.4). After drying for 60 min at 37°C, radioactivity was measured in scintillation liquid in a Packard Tri-Ca…


Reference Title:
Engineering Cyclic Amidases for Non-natural Amino Acid Synthesis
Reference Date:
2012
Citation:
Cite this protocol as: Heras-Vázquez F.J.L., Clemente-Jiménez J.M., Martínez-Rodríguez S., Rodríguez-Vico F. (2012) Engineering Cyclic Amidases for Non-natural Amino Acid Synthesis. In: Pollegioni L., Servi S. (eds) Unnatural Amino Acids. Methods in Molecular Biology (Methods and Protocols), vol 794. Humana Press
Product Usage:
…Gel Cutting Tips: GelX6.5 tips for 1 mL pipette (Cleaver Scientific Ltd., Warwickshire, UK).…


Reference Title:
Scaling-Up of Natural Products Isolation
Reference Date:
2012
Citation:
Cite this protocol as: Kumarasamy Y. (2012) Scaling-Up of Natural Products Isolation. In: Sarker S., Nahar L. (eds) Natural Products Isolation. Methods in Molecular Biology (Methods and Protocols), vol 864. Humana Press
Product Usage:
… useful feature of this system is that it is available with interchangeable vessels which range from 2.2 L to 14.0 L. Other companies that produce similar fermentors include Minifors®, Lavalabs®, and Cleaver Scientific. Bench-top fermenters are usually cheaper to purchase than the trolley mounted or skid-mounted fermenters. The entry level fermentors are simple in construction and can be thought o…


Reference Title:
Mycorrhizal symbiosis affected by different genotypes of Pinus pinaster
Reference Date:
2012
Citation:
Sousa, N.R., Ramos, M.A., Franco, A.R. et al. Plant Soil (2012) 359: 245. https://doi.org/10.1007/s11104-012-1196-5
Product Usage:
…he gel was soaked for 10 min in 3?×?GelGreen™ (Invitrogen), 1 min in deionised water and immediately photographed under a blue light transillumination table with a camera module (CSL-Microdoc System, Cleaver Scientific, UK). DGGE banding data were used to estimate diversity (H) (Shannon and Weaver 1963) and equitability (E) (Pielou 1975) indexes. DGGE profiles, concerning the presence and intensit…


Reference Title:
Pandemic (H1N1) 2009 influenza virus induces weaker host immune responses in vitro: a possible mechanism of high transmissibility
Reference Date:
2011
Citation:
Mukherjee, S., Vipat, V.C., Mishra, A.C. et al. Virol J (2011) 8: 140. https://doi.org/10.1186/1743-422X-8-140
Product Usage:
…0 µg) from cell extracts were separated by 12.5% SDS-polyacrylamide gel electrophoresis (12.5% SDS-PAGE) and transferred to Hybond-C (Amersham Biosciences) membrane with an electrotransfer apparatus (Cleaver Scientific Ltd) at 10 Volts (100 mA) for 1 h 30 min. Primary and secondary antibody interaction was performed in phosphate-buffered saline (pH 7.5). Primary antibodies used were rabbit anti-ST…


Reference Title:
Metabolic and endocrine profiles and reproductive parameters in dairy cows under grazing conditions: effect of polymorphisms in somatotropic axis genes
Reference Date:
2011
Citation:
Ruprechter, G., Carriquiry, M., Ramos, J.M. et al. Acta Vet Scand (2011) 53: 35. https://doi.org/10.1186/1751-0147-53-35
Product Usage:
… resolved in a 2% agarose gel stained with ethidium bromide (EtBr) and fragments of either leucine (L; 265, 96, 51 and 16 bp) or valine (V; 265, 147 and 16 bp) alleles were visualized under UV light (Cleaver Scientific, England).…


Reference Title:
Heat-shock protein 90 alpha (HSP90a) modulates signaling pathways towards tolerance of oxidative stress and enhanced survival of hepatocytes of Mugil cephalus
Reference Date:
2011
Citation:
Padmini, E. & Usha Rani, M. Cell Stress and Chaperones (2011) 16: 411. https://doi.org/10.1007/s12192-011-0255-9
Product Usage:
… µm pore size (Pall Corporation, East Hills, New York, USA) at 15 V for 90 min using alkaline buffer (48 mM Tris, 39 mM glycine, 0.037% w/v SDS, 20% v/v methanol, pH 8.3) and semi-dry blotting units (Cleaver Scientific Ltd., Rugby, UK). The PVDF blots were blocked with TBST (10 mM Tris, 150 mM NaCl, 1.54 mM sodium azide, 0.05% v/v Tween-20, pH 7.5) containing 5% w/v skimmed milk powder for 3 h, fo…


Reference Title:
Local RNA Silencing Mediated by Agroinfiltration
Reference Date:
2011
Citation:
Cite this protocol as: Helm J.M., Dadami E., Kalantidis K. (2011) Local RNA Silencing Mediated by Agroinfiltration. In: Kodama H., Komamine A. (eds) RNAi and Plant Gene Function Analysis. Methods in Molecular Biology (Methods and Protocols), vol 744. Humana Press
Product Usage:
…Blotting: SD20 Semi Dry Midi (Cleaver Scientific Ltd, Warwickshire, UK).…


Reference Title:
The chemopreventive effect of Ginkgo biloba and Silybum marianum extracts on hepatocarcinogenesis in rats
Reference Date:
2011
Citation:
El Mesallamy, H.O., Metwally, N.S., Soliman, M.S. et al. Cancer Cell Int (2011) 11: 38. https://doi.org/10.1186/1475-2867-11-38
Product Usage:
…riton X-100 and 10% DMSO for 24 h at 4°C. After lysis, the slides were rinsed three times in deionized water to remove salt and detergent. The slides were placed in a horizontal electrophoresis unit (Cleaver Scientific Ltd, UK) and DNA was allowed to unwind for 20 min in alkaline solution containing 300 mM NaOH and 1 mM EDTA, pH > 13. The DNA was electrophoresed for 20 min at 300 mA and 30 V (0.90…


Reference Title:
Expression and structural characterization of peripherin/RDS, a membrane protein implicated in photoreceptor outer segment morphology
Reference Date:
2010
Citation:
Vos, W.L., Vaughan, S., Lall, P.Y. et al. Eur Biophys J (2010) 39: 679. https://doi.org/10.1007/s00249-009-0553-7
Product Usage:
…Proteins were transferred to PVDF membrane (Amersham) using a transfer unit for semi-dry transfer (Cleaver Scientific) according to the manufacturer’s instructions. Western blots were probed with c-myc tag mouse monoclonal antibody (Cell Signaling), using anti-mouse HRP conjugated antibody as the secondary (Promega). Mass spectrometry analysis was performed on a an LC/MS system consisting of a 1,2…