SpotQuest – 2D Gel Analysis Software


Key Features

  • Simple analysis software to pick-up and use without training.
  • Same workflow to analyse single-stain and DIGE approach
  • Make your 2D-gel based protein analysis results accurate, sensitive and reliable.
  • Easy to learn, demonstrate and supply to users across all territories.
  • Economical solution for occasional use of 2D gels within your laboratory.
  • Windows Operating Systems, (7, 8 & 10 32-bit and 64-bit) and Windows tablets.


GTIN: 5055323272721

Special Requirements?


SpotQuest is software with basic functionality and easy to use 10-step workflow. It makes it easy for small laboratories and novice users to apply 2D gel electrophoresis as part of molecular biology or biochemistry research. It gives you a quick, simple, objective way to detect and measure changes in protein samples using 2D gel image analysis.

  • 3 minutes to fully analyse 1,000 proteins on each gel image with no need for tedious, subjective spot detection and matching.

A 10-step sequence takes you from opening your 2D gel images to generating a map of differentially expressed proteins without setting parameters.

  • A guided automatic workflow means new or occasional users can generate consistent and reliable results every time.

SpotQuest features

  • Simple download and activate with an e-licence
  • Analyses single stain and DIGE experiments
  • Supports .tiff, .img, .png, .gel, .mel images
  • Automatically performs a range of image quality checks and provides feedback
  • Image manipulation tools include rotate, flip, invert and crop
  • Ability to exclude areas of the gel image from the analysis
  • Allows a highly accurate correction of image distortion through the placement of alignment vectors
  • Alignment review step allows adjustments if needed
  • Automatic analysis of aligned image set
    • Spot detection
    • Background subtraction
    • Normalisation
    • 100% matching of all spots across the experiment
  • Filter out spots or areas of the gel image to be excluded from further analysis
  • Group gel images according to experiment structure and spot fold change and ANOVA p-value displayed in review results step.
  • Review of spots using 2D / 3D montage view and spot data table
  • Spot edits on a single gel image copied to all other gel images in the experiment
  • Expression profile plot with error bar showing 3 standard errors within groups
  • Option to export spot measurements as .csv file
  • Spots for picking highlighted on the image view to guide manual picking
  • Report shows an overview of spots selected for picking

Catalogue Page Number: 67