Cleaver Scientific IPG Strips pH Range 6-10

£101.49£169.15
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Key Features

  • A rigid plastic backing supports a covalently bound gel matrix to prevent breakage and make handling easier
  • Each strip is supplied freeze-dried and with a detachable cover film to stop contamination
  • Strips pre-printed with their own lot number for full traceability, documentation and GMP/GLP compliance
  • The anodic end is labelled as +, while the cathodic end is light blue in colour to ensure that the strips are positioned in the correct polar orientation before first-dimension focusing and second-dimension PAGE
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Product Description

Cleaver Scientific IPG strips are available in 3 different strips lengths to fit all Cleaver Scientific vertical systems for second-dimension PAGE. Each strip length is offered in a wide selection of pH gradients, with 3, 4 and 7 pH units per strip, to maximise the resolving power of the CSLIEF flat-bed isoelectric focusing system. Shorter strips are normally used to develop and validate methods in the omniPAGE CVS10 mini-gel system*, whereas longer strips of 18cm lengths are best employed in the VS20 WAVE maxi system, with its IPG converter kit, to optimise separation and resolution with higher protein loads. 24cm IPG strips are recommended for use in the VS30 second-dimension vertical system. Cleaver Scientific IPG strips are cast using high quality ampholytes, and undergo stringent QC tests for quality and 2-D gel-to-gel reproducibility.

*May also be used with VS10WDSYS with its IPG converter kit.

Design benefits offered by Cleaver Scientific IPG strips include:

  • A rigid plastic backing that supports a covalently bound gel matrix to prevent breakage and make handling easier
  • Each strip is supplied freeze-dried and with a detachable cover film to stop contamination
  • Strips pre-printed with their own lot number for full traceability, documentation and GMP/GLP compliance
  • The anodic end is labelled as +, while the cathodic end is light blue in colour to ensure that the strips are positioned in the correct polar orientation before first-dimension focusing and second-dimension PAGE
  • Light blue stain migrates from cathode to anode during focusing, serving as a marker for electrophoresis progression
  • Sample-loading by overnight rehydration or cup-loading

Gradient selection criteria:

  • Supplied in 7, 18 and 24cm strip lengths with 6 different gradients
  • Standard broad-range pH gradients maximise resolution within a single second-dimension gel (e.g. pH 3-10)
  • Narrow-range gradients (e.g. pH 3-6) optimise resolving power within a specific pH region by offering more centimetres of gel per pH unit
  • pH 3-10 Non Linear gradient strips have a flat zone corresponding to pH 5-7 for increased protein resolving power within this region

Quality assured:

  • Consistency – all 12 strips supplied with each package come from the same production lot
  • Reliability –  accurate pH gradient casting procedures ensure lot-to-lot and 2-D gel-to-gel reproducibility
  • May be used with all commercial IEF systems

Ordering Information

CSL-IPG6-10/7  7cm IPG Strip Length
CSL-IPG6-10/18  18cm IPG Strip Length
CSL-IPG6-10/24  24cm IPG Strip Length

 

Technical Specification

IPG Strip Length 7cm 18cm 24cm
Width/Gel Thickness 3mm/0.5mm 3mm/0.5mm 3mm/0.5mm
pH Gradients Narrow Range Narrow Range Narrow Range
Storage Temperature/Stability -20C / 18 months -20C / 18 months -20C / 18 months
Recommended Protein Load 5-100g 50-300g 80-700g
Recommended 2D vertical systems (strip capacity) CVS10DSYS (2), VS10WDSYS with IPG Converter kit (4) VS20WAVE with IPG converter Kit (2) VSD30DSYS (2)